Abstract
Laguna de la Cruz (Lake La Cruz ) is a small karstic meromictic lake located in Central-Eastern Spain. Its biogenic meromixis (originated ca.1660) is favoured by the high relative depth and the steep doline walls. Calcium and magnesium bicarbonate are the main salts. The monimolimnion permanently spans from 18 m to the lake bottom (ca.21 m), whereas thermal stratification occurs above from April to October. Steep chemical gradients appear both at the bottom of the metalimnion (seasonally) and, permanently, at the permanent chemocline (16–18 m). Nutrients in surface waters decay as stratification advances, but deeper they are abundant. Linked to physical and chemical gradients, sharply stratified planktonic phototrophic populations develop, with the highest rates of inorganic carbon fixation occurring at the oxic–anoxic interface during the stratification period. In the oxic and microaerobic layers, the photosynthetic planktonic assemblage is dominated by picocyanobacteria , seasonally forming a deep chlorophyll maximum. Instead, photosynthetic sulphur bacteria thrive in anoxic waters. Chemolithoautotrophic bacteria, some linked to the iron cycle , show rates of dark inorganic carbon fixation that overpass the contribution of anoxygenic photosynthesis to total carbon fixation in the lake, though this is far dominated by oxygenic photosynthesis . Most of the deep primary production sinks to anoxic waters being unconsumed by the metazooplankton dominated by rotifers and the copepod Tropocyclops prasinus. Only a few species of anaerobic ciliates consume part of this sinking organic matter , which is anaerobically decomposed in the anoxic layers or reaches the lake sediments annually forming a dark layer that alternates with a white layer. This white layer results from massive precipitation of calcium carbonate (whiting ) yearly occurring in summer. Remains of organisms and minerals in Lake La Cruz sediments serve as palaeoecological archives, allowing to describe the past ecological conditions of the lake.
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References
Ambrosetti W, Barbanti L (2005) Evolution towards meromixis of Lake Iseo (Northern Italy) as revealed by its stability trend. J Limnol 64:1–11
Armengol X (1997) Caracterización estructural del zooplancton de las lagunas cársticas de Cuenca, con especial atención a su distribución vertical. PhD Dissertation, Universitat de València
Armengol X, Miracle MR (1999) Zooplankton communities in doline lakes and pools, in relation to some bathymetric parameters and physical and chemical variables. J Plankton Res 21:2245–2261
Armengol X, Miracle MR (2000) Diel vertical movements of zooplankton in Lake La Cruz (Cuenca, Spain). J Plankton Res 22:1683–1703
Armengol-Díaz J, Esparcia A, Vicente E et al (1993) Vertical distribution of planktonic rotifers in a karstic meromictic lake. Hydrobiologia 255(256):381–388
Armengol X, Wurtsbaugh WA, Camacho A et al (2012) Pseudo-diel vertical migration in zooplankton: a whole-lake 15N tracer experiment. J Plankton Res 34:976–986
Ávila A, Burrel JL, Domingo A et al (1984) Limnology of the Lago Grande de Estanya (Huesca) (Limnologia del Lago Grande de Estanya (Huesca)). Oecologia Aquat 7:3–24
Barbosa FA, Padisák J (2002) The forgotten lake stratification pattern: atelomixis, and its ecological importance. Verh Int Ver Theor Angew Limnol 28:1385–1395
Barreiro-Lostres F, Moreno A, Giralt S et al (2014) Climate, palaeohydrology and land use change in the Central Iberian Range over the last 1.6 kyr: the La Parra Lake record. Holocene 24:1177–1192
Barriendos M, Llasat MC (2003) The case of the Maldá anomaly in the Western Mediterranean basin (AD 1760–1800): an example of a strong climatic variability. Climatic Change 61:191–216
Bathurst RG (1971) Carbonate sediments and their diagenesis. Developments in sedimentology, 12. Elsevier, Amsterdam
Bertilsson S, Jones JB (2003) Supply of dissolved organic matter to aquatic ecosystems: autochthonous sources. In: SEG F, RL S (eds) Aquatic ecosystems: interactivity of dissolved organic matter, Aquatic Ecology Series, 1st edn. Academic, San Diego, pp 26–70
Botella y de Hornos F (1875) La ciudad encantada. Las hoces, salegas y torcas de la provincia de Cuenca. Anales Soc Esp Hist Nat 4:233–239
Boronat L (2003) Distribución de los microcrustáceos en Lagunas de Castilla—La Mancha. Ciclos estacionales y migración vertical en lagunas cársticas estratificadas. Dissertation, Universitat de València
Boronat L, Miracle MR, Armengol X (2001) Cladoceran assemblages in a mineralization gradient. Hydrobiologia 442:75–88
Bradbury P, Cumming B, Laird K (2002) A 1500-year record of climatic and environmental change in Elk Lake, Minnesota III: measures of past primary productivity. J Paleolimnol 27:321–340
Brunskill GJ (1969) Fayetteville Green Lake, New York. II: precipitation and sedimentation of calcite in a meromictic lake with laminated sediments. Limnol Oceanogr 14:830–847
Camacho A (2006) On the occurrence and ecological features of deep chlorophyll maxima (DCM) in Spanish stratified lakes. Limnetica 25:453–478
Camacho A (2009) Sulfur bacteria. In: Likens GE (ed) Encyclopedia of inland waters. Elsevier Academic Press, Oxford, New York, pp 261–278
Camacho A, Vicente E (1998) Carbon photoassimilation by sharply stratified phototrophic communities at the chemocline of Lake Arcas (Spain). FEMS Microbiol Ecol 25:11–22
Camacho A, Erez J, Chicote A et al (2001) Microbial microstratification, inorganic carbon photoassimilation and dark carbon fixation at the chemocline of the meromictic Lake Cadagno (Switzerland) and its relevance to the food web. Aquat Sci 63:91–106
Camacho A, Picazo A, Miracle MR et al (2003a) Spatial distribution and temporal dynamics of picocyanobacteria in a meromictic karstic lake. Algological Stud 109:171–184
Camacho A, Wurtsbaugh WA, Miracle MR et al (2003b) Nitrogen limitation of phytoplankton in a Spanish karst lake with a deep chlorophyll maximum: a nutrient enrichment bioassay approach. J Plankton Res 25:397–404
Camacho A, Miracle MR, Vicente E (2003c) Which factors determine the abundance and distribution of picocyanobacteria in inland waters? A comparison among different types of lakes and ponds. Arch Hydrobiol 157:321–338
Campbell P, Torgersen T (1980) Maintenance of iron meromixis by iron redeposition in a rapidly flushed monimolimnion. Can J Fish Aquat Sci 37:1303–1313
Camuffo D, Secco C, Brimblecombe P et al (2000) Sea storms in the Adriatic Sea and the Western Mediterranean during the last millennium. Climatic Change 46:209–223
Canfield DE, Farquhar J, Zerkle AL (2010) High isotope fractionations during sulphate reduction in a low-sulphate euxinic ocean analog. Geology 38:415–418
Casamayor EO, Llirós M, Picazo A et al (2012) Contribution of deep dark fixation processes to overall CO2 incorporation and large vertical changes of microbial populations in stratified karstic lakes. Aquat Sci 74:61–75
Casty C, Handorf D, Raible CC et al (2005) Recurrent climate winter regimes in reconstructed and modelled 500 hPa geopotential height fields over the North Atlantic/European sector 1659–1990. Clim Dynam 24:809–822
Cirujano S (1995) Flora y vegetación de las lagunas y humedales de la provincia de Cuenca. JCCLM—Real Jardín Botánico de Madrid (CSIC), Madrid
Cirujano S, Medina L (2002) Plantas acuáticas de las lagunas y humedales de Castilla-La Mancha. JCCLM—Real Jardín Botánico de Madrid (CSIC), Madrid
Culver DA, Brunskill GJ (1969) Fayetteville Green Lake. V. Studies of primary production and zooplankton in a meromictic marl lake. Limnol Oceanogr 14:862–873
Dasí MJ, Miracle MR (1991) Distribución vertical y variación estacional del fitoplancton de una laguna cárstica meromíctica, la Laguna de la Cruz (Cuenca, España). Limnetica 7:37–59
De Vicente I, Cattaneo K, Cruz-Pizarro L et al (2006) Sedimentary phosphate fractions related to calcite precipitation in a eutrophic hardwater lake (Lake Alserio, Northern Italy). J Paleolimnol 35:55–64
Dokulil MT, Kaiblinger C (2009) Phytoplankton productivity. In: Likens GE (ed) Encyclopedia of inland waters, vol 1. Elsevier, Oxford, pp 210–218
Eraso AE, López-Azevedo V, López M et al (1979) Estudio de Las Torcas de Palancares y Cañada del Hoyo en el karst de la Serranía de Cuenca. Kobie 9:7–79
Esparcia A (1993) Distribuciones de las poblaciones de rotíferos en la oxiclina de La Laguna de La Cruz. Adaptaciones metabólicas a la microaerofilia en Brachionus plicatilis. PhD Dissertation, Universitat de València
Esparcia A, Armengol J, Vicente E et al (1991) Vertical distribution of Anuraeopsis species as related to oxygen depletion in two stratified lakes. Verh Int Ver Theor Angew Limnol 24:2745–2749
Esparcia A, Armengol J, Miracle MR (2001) Relación de la distribución de los rotíferos con la de los principales factores físicos y químicos en la laguna de la Cruz. Limnetica 20:305–320
Folk RL (1974) The natural history of crystalline calcium carbonate: effect of magnesium content and salinity. J Sediment Res 44(1):50–53
Font-Tullot I (1988) Historia del clima de España. Cambios climáticos y sus causas. Instituto Nacional de Meteorología, Madrid
Galat DL, Jacobsen RL (1985) Recurrent aragonite precipitation in saline-alkaline Pyramid Lake, Nevada. Arch Hydrobiol 105:137–159
Gruber N, Wehrli B, Wüest A (2000) The role of biogeochemical cycling for the formation and preservation of varved sediments in Soppensee (Switzerland). J Paleolimnol 24:277–291
Hofmann W (1982) On the coexistence of two pelagic Filinia species (Rotatoria) in Lake Plussee. I. Dynamics of abundance and dispersion. Arch Hydrobiol 95:125–137
Hurrell JW (1995) Decadal trends in North Atlantic Oscillation and relationship to regional temperature and precipitation. Science 269:676–679
Hutchinson GE (1957) A treatise on Limnology, Geography, physics and chemistry, vol I. Willey, New York
Imboden DM, Wüest A (1995) Mixing mechanisms in lakes. In: Leman A, Imboden DM, Gat JR (eds) Physics and chemistry of lakes, 1st edn. Springer, Berlin, pp 83–138
Jankowski T, Livingstone DM, Bührer H et al (2006) Consequences of the 2003 European heat wave for lake temperature profiles, thermal stability, and hypolimnetic oxygen depletion: implications for a warmer world. Limnol Oceanogr 51:815–819
Jersabek CD, Koste W (1993) Additional notes on taxonomy and ecology of Anuraeopsis miracleae Koste, 1991 (Rotatoria: Monogononta) from an Austrian alpine lake. Hydrobiologia 264:55–60
Julià R, Burjachs F, Dasí MJ et al (1998) Meromixis origin and recent trophic evolution in the Spanish mountain lake La Cruz. Aquat Sci 60:279–299
Kelts K, Hsü KJ (1978) Freshwater carbonate sedimentation. In: Lerman A (ed) Lakes. Geology, Chemistry, Physics, 1st edn. Springer, New York, pp 295–323
Kiss KT, Acs E, Szabó KE et al (2007) Morphological observations on Cyclotella distinguenda Hustedt and C. delicatula Hustedt from the core sample of a meromictic karstic lake of Spain (Lake La Cruz) with aspects of their ecology. Diatom Res 22:287–308
Koschel R, Benndorf J, Proft G et al (1983) Calcite precipitation as a natural control mechanism of eutrophication. Arch Hydrobiol 98:380–408
Koste W (1980) Uber zwei Plankton-Radertiertaxa Filinia australiensis n sp and Filinia hofmanni n sp, mit Bermerkun- gen zur Taxonomie der Longiseta terminalis Gruppe . Genus Filinia Bory de St. Vincent, 1824, Familie Filiniidae Bartos 1959 (Ubeordnung Monogononta). Arch Hydrobiol 90:230–256
Koste W (1991) Anuraeopsis miraclei, a new planktonic rotifer species in karstic lakes of Spain. Hydrobiologia 209:169–173
Lean J (2000) Evolution of the Sun’s spectral irradiance since the Maunder Minimum. Geophys Res Lett 27:2425–2428
Leavitt PR, Carpenter SR (1990) Aphotic pigment degradation in the hypolimnion: implications for sedimentation studies and paleolimnology. Limnol Oceanogr 35:520–534
Leavitt PR, Hodgson DA (2001) Practical methods for analysis of sedimentary pigment. In: Smol JP, Birks HJB, Last WM (eds) Developments in paleoenvironmental research, tracking environmental changes using lake sediments, terrestrial, algal and siliceous indicators, vol 3. Kluwer, Dordrecht, pp 295–325
Lehours AC, Evans P, Bardot C et al (2007) Phylogenetic diversity of archaea and bacteria in the anoxic zone of a meromictic lake (Lake Pavin, France). Appl Environ Microb 73:2016–2019
Luterbacher J, Xoplaki E, Dietrich D et al (2001) Extending North Atlantic oscillation reconstructions back to 1500. Atmos Sci Lett 2:114–124
Margalef R (1983) Limnología. Omega, Barcelona
Marra J (2009) Net and gross productivity: weighing in with 14C. Aquat Microb Ecol 56:123–131
McDonough RJ, Sanders RW, Porter KG et al (1986) Depth distribution of bacterial production in a stratified lake with an anoxic hypolimnion. Appl Environ Microb 52:992–1000
Mezquita F, Miracle MR (1997) Chydorid assemblages in the sedimentary sequence of Lake La Cruz (Spain) subject to water level changes. In: Brancelj A, De Meester L, Spaak P (eds) Cladocera: the biology of model organisms. Springer, Netherlands, pp 277–285
Mezquita F, Boronat MD, Miracle MR (2002) The life history of Cyclocypris ovum (Ostracoda) in a permanent karstic lake. Arch Hydrobiol 155:687–704
Miracle MR, Vicente E (1983) Vertical distribution and rotifer concentrations in the chemocline of meromictic lakes. Hydrobiologia 104:259–267
Miracle MR, Vicente E, Pedrós-Alió C (1992) Biological studies of Spanish meromictic and stratified karstic lakes. Limnetica 8:59–77
Miracle MR, Alfonso T (1993) Rotifer vertical distributions in a meromictic basin of Lake Banyoles (Spain). Hydrobiologia 255/256:371–380
Miracle MR, Dasí MJ, Vicente E (1998) Forced phytoplankton vertical migrations due to lake water “whiting”. Verh Int Ver Theor Angew Limnol 26:1749–1754
Miracle MR, Camacho A, Julià R et al (2000) Sinking processes and their effect on the sedimentary record in the meromictic Lake La Cruz (Spain). Verh Int Ver Theor Angew Limnol 27:1209–1213
Modamio X, Pérez V, Samarra F (1988) Limnologia del Lago de Montcortes (ciclo 1978–79), (Pallars-Jussa, Lleida). Oecologia Aquat 9:9–17
Mopper K, Schultz CA (1993) Fluorescence as a possible tool for studying the nature and water column distribution of DOC components. Mar Chem 41:229–238
Morata SM, Camacho A, Miracle MR et al (2003) Asociaciones fitoplanctónicas y su periodicidad en un lago marcadamente estratificado. Limnetica 22:35–52
Müller G (1971) Aragonite inorganic precipitation in a freshwater lake. Nature 229:18–18
Müller G, Irion G, Foerstner U (1972) Formation and diagenesis of inorganic Ca-Mg carbonates in the lacustrine environment. Naturwissenschaften 59:158–164
Ojala AEK, Francus P, Zolitschka B et al (2012) Characteristics of sedimentary varve chronologies—a review. Quat Sci Rev 43:45–60
Orem WH, Burnett WC, Landing WM et al (1991) Jellyfish Lake, Palau: early diagenesis of organic matter in sediments of an anoxic marine lake. Limnol Oceanogr 36:526–543
Parkin TB, Brock TD (1980) The effects of light quality on the growth of phototrophic bacteria in lakes. Arch Microbiol 125:19–27
Pauling A, Luterbacher J, Casty C et al (2006) Five hundred years of gridded high-resolution precipitation reconstructions over Europe and the connection to large-scale circulation. Clim Dynam 26:387–405
Peck JA, Khosbayar P, Fowell SJ et al (2002) Mid to Late Holocene climate change in north central Mongolia as recorded in the sediments of Lake Telmen. Palaeogeogr Palaeoecol 183:135–153
Picazo A, Rochera C, Vicente E et al (2013) Spectrophotometric methods for the determination of photosynthetic pigments in stratified lakes: a critical analysis based on comparisons with HPLC determinations in a model lake. Limnetica 32:139–158
Planas D (1973) Composición, ciclo y productividad del fitoplancton del lago de Banyoles. Oecologia Aquat 1:3–106
Reynolds CS (2006) The ecology of phytoplankton. Cambridge University Press, Cambridge
Roca JR, Mezquita F, Rueda J et al (2000) Endorheic versus karstic lakes: patterns of ostracod distributions and lake typology in a Mediterranean landscape (Castilla-La Mancha, Spain). Mar Freshw Res 51:311–319
Rodrigo MA (1997) Limnología comparada de las lagunas de dos sistemas cársticos de Cuenca: Bacterias fotosintéticas de la Laguna de La Cruz y la Laguna de Arcas-2. PhD Dissertation, Universitat de València
Rodrigo MA, Vicente E, Miracle MR (1993) Short-term calcite precipitation in the karstic meromictic Lake La Cruz (Cuenca, Spain). Verh Int Ver Theor Angew Limnol 25:711–719
Rodrigo MA, Vicente E, Miracle MR (2000) The role of light and concentration gradients in the vertical stratification and seasonal development of phototrophic bacteria in a meromictic lake. Arch Hydrobiol 148:533–548
Rodrigo MA, Miracle MR, Vicente E (2001) The meromictic Lake La Cruz (Central Spain). Patterns of stratification. Aquat Sci 63:406–416
Rodriguez-Puebla C, Encinas AH, Nieto S et al (1998) Spatial and temporal patterns of annual precipitation variability over the Iberian Peninsula. Int J Climatol 18:299–316
Rojo C, Miracle MR (1987) Poblaciones fitoplanctónicas de La Laguna de La Cruz (Cuenca), una laguna cárstica meromíctica. Act. VI Simp Nat Bot Criptogámica, pp 119–135
Romero L, Camacho A, Vicente E et al (2006) Sedimentation patterns of photosynthetic bacteria based on pigment markers in meromictic Lake La Cruz (Spain): paleolimnological implications. J Paleolimnol 35:167–177
Romero-Viana L (2007) Paleolimnological study in two karstic lakes: climate signal in varved sediment and phototrophic organisms variability. Dissertation, Universitat de València
Romero-Viana L, Julià R, Camacho A et al (2008) Climate signal in varve thickness: Lake La Cruz (Spain), a case study. J Paleolimnol 40:703–714
Romero-Viana L, Miracle MR, López-Blanco C et al (2009) Sedimentary multiproxy response to hydroclimatic variability in Lagunillo del Tejo (Spain). In: Buczkó K, Korponai J, Padisák J, Starratt SW (eds) Palaeolimnological Proxies as Tools of Environmental Reconstruction in Fresh Water. Developments in Hydrobiology. Springer, The Netherlands, pp 231–245
Romero-Viana L, Keely BJ, Camacho A et al (2010) Primary production in Lake La Cruz (Spain) over the last four centuries: reconstruction based on sedimentary signal of photosynthetic pigments. J Paleolimnol 43:771–786
Romero-Viana L, Julià R, Schimmel M et al (2011) Reconstruction of annual winter rainfall since AD 1579 in central-eastern Spain based on calcite laminated sediment from Lake La Cruz. Climatic Change 107:343–361
Rossknecht H (1980) Phosphatelimination durch autochthone Calcitfällung im Bodensee-Obersee. Arch Hydrobiol 88:328–344
Schmidt R, Psenner R, Müller J et al (2002) Impact of late glacial climate variations on stratification and trophic state of the meromictic lake Längsee (Austria): validation of a conceptual model by multi proxy studies. J Limnol 61:49–60
Sendra MD (2009) Distribución del fitoplancton en la laguna de la cruz y su relación con los nutrientes. Dissertation, Universitat de València
Sommer U, Gliwicz ZM, Lampert W et al (1986) The PGE-model of seasonal succession of planktonic events in fresh waters. Arch Hydrobiol 106:433–471
Starkweather PL, Miracle MR, Vicente E et al (2005) Diel distribution and migration of Chaoborus flavicans: a field analysis using broadband Acoustic Doppler Current Profiler (ADCP) techniques. Verh Int Ver Theor Angew Limnol 29:672–677
Touchan R, Xoplaki E, Funkhouser G et al (2005) Reconstructions of spring/summer precipitation for the Eastern Mediterranean from tree-ring widths and its connection to large-scale atmospheric circulation. Clim Dynam 25:75–98
Valero-Garcés B, Morellón M, Moreno A et al (2014) Lacustrine carbonates of Iberian Karst Lakes: sources, processes and depositional environments. Sediment Geol 299:1–29
Van Gemerden H, Mas J (2004) Ecology of phototrophic sulphur bacteria. In: Blankenship RE, Madigan MT, Bauer CE (eds) Advances in photosynthesis and respiration, anoxygenic photosynthetic bacteria, Part I, vol 2. Springer, Dordrecht, pp 49–85
Vanderploeg HA, Eadie BJ, Liebig JR et al (1987) Contribution of calcite to the particle-size spectrum of Lake Michigan seston and its interactions with the plankton. Can J Fish Aquat Sci 44:1898–1914
Vicente E, Miracle MR (1988) Physicochemical and microbial stratification in a meromictic karstic lake of Spain. Verh Int Ver Theor Angew Limnol 23:522–529
Vila X, Abella CA (1994) Effects of light quality on the physiology and the ecology of planktonic green sulphur bacteria in lakes. Photosynth Res 41:53–65
Vila X, Abella CA, Figueras JB et al (1998) Vertical models of phototrophic bacterial distribution in the metalimnetic microbial communities of several freshwater North-American kettle lakes. FEMS Microbiol Ecol 25:287–299
Walker KF, Likens GE (1975) Meromixis and a reconsidered typology of lake circulation patterns. Verh Int Ver Theor Angew Limnol 19:442–458
Walter XA (2011). Anaerobic iron cycling in a neoarchean ocean analogue. Dissertation, Université de Neuchâtel
Walter XA, Picazo A, Miracle MR et al (2014) Phototrophic Fe(II)-oxidation in the chemocline of a ferruginous meromictic lake. Front Microbiol 5:713. doi:10.3389/fmicb.2014.00713
Weber KA, Picardal FW, Roden EE (2001) Microbially catalyzed nitrate-dependent oxidation of biogenic solid-phase Fe (II) compounds. Environ Sci Technol 35:1644–1650
Weidemann AD, Bannister TT, Effler SW, Johnson DL (1985) Particulate and optical properties during CaCO3 precipitation in Otisco Lake. Limnol Oceanogr 30:1078–1083
Wetzel RG (2001) Limnology: lake and river ecosystems. Academic, San Diego
Wright SW, Jeffrey SW, Mantoura RFC (eds) (2005) Phytoplankton pigments in oceanography: guidelines to modern methods. UNESCO, Paris
Wüest A, Aeschbach-Hertig W, Baur H et al (1992) Density structure and tritium-helium age of deep hypolimnetic water in the northern basin of Lake Lugano. Aquat Sci 54:205–218
Xoplaki E, Maheras P, Luterbacher J (2001) Variability of climate in meridional Balkans during the periods 1675–1715 and 1780–1830 and its impact on human life. Climatic Change 48:581–615
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Camacho, A., Miracle, M.R., Romero-Viana, L., Picazo, A., Vicente, E. (2017). Lake La Cruz, an Iron-Rich Karstic Meromictic Lake in Central Spain. In: Gulati, R., Zadereev, E., Degermendzhi, A. (eds) Ecology of Meromictic Lakes. Ecological Studies, vol 228. Springer, Cham. https://doi.org/10.1007/978-3-319-49143-1_8
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