Abstract
The selection of a reliable UDAF, Class II or III Cabinet, Isolette, or workstation, that meets the needs of the laboratory staff and which protects and meets every requirement of gametes and embryos, can become a daunting task. There are many products on the market to select from, and in this chapter, I will try to give you some insight in what you should look into and demand from the manufacturer when it comes to the quality and the function of the different parts of the product, their proven reliability, the company’s service agreements, and value for money. If the clinic can ensure an optimal environment for gametes and embryos during the different procedures, it will improve the outcome, which in turn generates a better reputation and a higher inflow of patients to the clinic.
For this to be accomplished, the clinic must proactively ensure that the couple’s gametes and embryos are given an optimal chance to develop into top-quality embryos with high implantation and low miscarriage rates.
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Review Questions
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1.
Why is it important to have perfect control of the temperature during egg collection, denudation of oocytes, and evaluation of embryos?
The meiotic spindle in oocytes is very sensitive to fluctuations in temperatures, which can cause aneuploidies and adverse effects on the embryo development.
Variations in temperatures can also cause developmental problems within embryos and lower implantation rates.
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2.
The engines of the ventilation system must be silent and not cause vibrations! Why?
Working in benches or a laboratory with equipment’s generating high noise levels give discomfort to staff and could also affect the work, if micro-manipulation techniques are implemented in the LAF/Class II.
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3.
A high-quality light source and a reliable temperature-controlled heated glass is one of many important features of a high-quality UDAF bench! Why?
Unreliable light source will affect the search for COCs, oocytes, and handling as well evaluation of embryos. Defect temperature control could affect the meiotic spindle, embryo development, and outcomes (IR).
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Johansson, L. (2019). What to Consider When Selecting a LAF, Class II Cabinet, or Isolette for Your ART Program?. In: Nagy, Z., Varghese, A., Agarwal, A. (eds) In Vitro Fertilization. Springer, Cham. https://doi.org/10.1007/978-3-319-43011-9_11
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DOI: https://doi.org/10.1007/978-3-319-43011-9_11
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