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In Vivo Preclinical Imaging of Developmental Biology

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Small Animal Imaging

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Abstract

Embryonic development, the generation of a living organism from a fertilized egg, poses some of the most intriguing challenges of biological research. Aberrations in development can arise from genetic alterations in the fetus, which can vary in the degree of penetration, and can also result from direct and indirect pathological processes affecting the fetus or the mother. Impaired fetal development is a major cause of premature morbidity and mortality. Dynamic imaging of the live fetus provides an important tool for elucidating the normal and pathological developmental changes occurring during pregnancy. In particular, as part of efforts for functional mapping of the genome using genetically modified animals, detailed analysis of fetal development in laboratory animals is central in elucidating the function of genes and the impact of alteration in gene expression. Moreover, imaging biomarkers developed in the context of basic biological research could provide the foundations for future prenatal clinical imaging. In this chapter, we will review recent developments in the use of noninvasive imaging for longitudinal monitoring of live embryos in small laboratory animals, with particular focus on in utero imaging of fetal development in the mouse.

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Acknowledgments

We acknowledge the support of the Israel Science Foundation ISF 93/07 and ISF Converging Technologies Equipment Award, the European Commission Seventh Framework ERC Advanced Project IMAGO 232,640, the US-Israel Binational Science Foundation and the National Institutes of Health (grant 1R01HD086323-01). MN is incumbent of the Helen and Morris Mauerberger Chair.

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Authors and Affiliations

  1. Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, NL-5600 MB, The Netherlands

    Katrien Vandoorne

  2. Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, Rehovot, 76100, Israel

    Tal Raz

  3. Sheba Cancer Research Center, Chaim Sheba Medical Center, Ramat Gan, 52621, Israel

    Stav Sapoznik

  4. Department of Veterinary Resources, Weizmann Institute, Rehovot, 76100, Israel

    Inbal E. Biton

  5. Biomedical Magnetic Resonance Laboratory, Mallinckrodt Institute of Radiology, Washington University, St. Louis, MO, 63110, USA

    Joel R. Garbow

  6. Department of Biological Regulation, Weizmann Institute, Rehovot, 76100, Israel

    Michal Neeman

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  1. Katrien Vandoorne
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  2. Tal Raz
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Correspondence to Michal Neeman .

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  1. Universitätsklinikum Aachen, Aachen, Germany

    Fabian Kiessling

  2. Universitätsklinikum Tübingen, Tübingen, Germany

    Bernd J. Pichler

  3. Bayer AG, Drug Discovery Pharmaceuticals, Berlin, Germany

    Peter Hauff

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Vandoorne, K., Raz, T., Sapoznik, S., Biton, I.E., Garbow, J.R., Neeman, M. (2017). In Vivo Preclinical Imaging of Developmental Biology. In: Kiessling, F., Pichler, B., Hauff, P. (eds) Small Animal Imaging. Springer, Cham. https://doi.org/10.1007/978-3-319-42202-2_25

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