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Perforins

  • Chapter
Electrophysiology of Unconventional Channels and Pores

Part of the book series: Springer Series in Biophysics ((BIOPHYSICS,volume 18))

Abstract

“Perforins” – cytotoxic cell perforin-1, proteins contributing to the complement membrane attack complex (MAC) and other perforin-like proteins – form unconventional pores in biological membranes in the sense that as well as a ring of subunits circumscribing an opening in the lipid bilayer, arcs of subunits induce similar effects. The resulting arc-pore structures are completed by a lipidic edge, conferring distinctive functional characteristics on them. Electrophysiological measurements have played a distinguished role in the discovery and characterisation of this alternative mechanism of pore formation which enables the same protein to form widely-varying sizes of pore and enact a greater diversity of effects than more conventional channels. This review discusses the mechanism of pore formation by the perforins and the way in which it has been illuminated by electrical conductance studies alongside imaging methods such as electron microscopy and atomic force microscopy.

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  1. Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford, OX3 7BN, UK

    Robert J. C. Gilbert

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  1. Robert J. C. Gilbert
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Correspondence to Robert J. C. Gilbert .

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  1. Department of Biology and Biochemistry, University of Houston, Houston, TX, USA

    Anne H. Delcour

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Gilbert, R.J.C. (2015). Perforins. In: Delcour, A.H. (eds) Electrophysiology of Unconventional Channels and Pores. Springer Series in Biophysics, vol 18. Springer, Cham. https://doi.org/10.1007/978-3-319-20149-8_12

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