Abstract
Ole e 5 is an olive pollen allergen displaying high identity with Cu,Zn superoxide dismutases. Previous studies characterized biochemical variability in this allergen, which may be of relevance for allergy diagnosis and therapy. The generation of an olive pollen transcriptome allowed us to identify eight Ole e 5 sequences, one of them including a 24 nt deletion. Further in silico analysis permitted designing primers for PCR amplification and cloning from both cDNA and gDNA. A large number of sequences were retrieved, which experimentally validated the predictive NGS sequences, including the deleted enzyme. The PCR-obtained sequences were used for further scrutiny, including sequence aligment and phylogenetic analysis. Two model sequences (a complete sequence and a deleted one) were used to perform 3-D modeling and a prediction of the T- and B-cell epitopes. These predictions interestingly foreseed relevant differences in the antigenicity/allergenicity of both molecules. Clinical relevance of differences is discussed.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Busquets, J., Cour, P., Guerin, B., Michel, F.B.: Busquets et al 1984 Allergy in the Mediterranean area_ I_ Pollen counts and pollinosis (1984)
Rodríguez, R., Villalba, M., Monsalve, R.I., Batanero, E.: The spectrum of olive pollen allergens. Int. Arch. Allergy Immunol. 125, 185–195 (2001)
Jimenez-Lopez, J.C., Rodriguez-Garcia, M.I.: Olive tree genetic background is a major cause of pofilin (Ole e 2 allergen) polymorphism and functional and allergenic variability. Commun. Agric. Appl. Biol. Sci. 78, 213–219 (2013)
Tejera, M.L., Villalba, M., Batanero, E.: Mechanisms of allergy Identification, isolation, and characterization of Ole e 7, a new allergen of olive tree pollen. Mech. Allergy 797–802 (1999)
Villalba, M., Rodríguez, R., Batanero, E.: The spectrum of olive pollen allergens. From structures to diagnosis and treatment. Methods 66, 44–54 (2014)
Batanero, E., Rodríguez, R., Villalba, M.: Olives and Olive Oil in Health and Disease Prevention. Elsevier (2010)
Boluda, L., Alonso, C., Fernández-Caldas, E.: Purification, characterization, and partial sequencing of two new allergens of Olea europaea. J. Allergy Clin. Immunol. 101, 210–216 (1998)
Butteroni, C., Afferni, C., Barletta, B., Iacovacci, P., Corintia, S., Brunetto, B., Tinghino, R., Ariano, R., Panzani, R.C., Pini, C., Di Felice, G.: Cloning and expression of the olea europaea allergen Ole e 5, the pollen Cu/Zn Superoxide Dismutase (2005)
Alché, J.D., Corpas, F.J., Rodríguez-García, M.I., Del Rio, L.A.: Identification and immunulocalization of SOD isoforms in olive pollen. Physiol. Plant 104, 772–776 (1998)
Parida, A.K., Das, A.B.: Salt tolerance and salinity effects on plants: a review. Ecotoxicol. Environ. Saf. 60, 324–349 (2005)
Alscher, R.G., Erturk, N., Heath, L.S.: Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. J. Exp. Bot. 53, 1331–1341 (2002)
Corpas, F.J., Fernández-Ocaña, A., Carreras, A., Valderrama, R., Luque, F., Esteban, F.J., Rodríguez-Serrano, M., Chaki, M., Pedrajas, J.R., Sandalio, L.M., del Río, L.A., Barroso, J.B.: The expression of different superoxide dismutase forms is cell-type dependent in olive (Olea europaea L.) leaves. Plant Cell Physiol. 47, 984–994 (2006)
Zafra, A., Traverso, J.A., Corpas, F.J., Alché, J.D.: Peroxisomal localization of CuZn superoxide dismutase in the male reproductive tissues of the olive tree. Microsc. Microanal. 18, 2011–2012 (2012)
Alché, J.D., Castro, A.J., Jiménez-López, J.C., Morales, S., Zafra, A., Hamman-Khalifa, A.M., Rodríguez-García, M.I.: Differential characteristics of olive pollen from different cultivars: biological and clinical implications. J. Investig. Allergol. Clin. Immunol. 17(Suppl 1), 17–23 (2007)
Zafra, A., Rodríguez-García, M.I., Alche, J.D.: Cellular localization of ROS and NO in olive reproductive tissues during flower development. BMC Plant Biol. (2010)
Iba, K.: Acclimative response to temperature stress in higher plants: approaches of gene engineering for temperature tolerance. Annu. Rev. Plant Biol. 53, 225–45 (2002)
Sambrook, J., Rusell, D.: Molecular Cloning: a laboratory manual. Cold Spring harbor Laboratory Press, New York (2001)
Falgueras, J., Lara, A.J., Fernández-pozo, N., Cantón, F.R., Pérez-trabado, G., Claros, M.G.: SeqTrim: a high-throughput pipeline for pre-processing any type of sequence read. BMC Bioinformatics (2010)
Gouy, M., Guindon, S., Gascuel, O.: SeaView version 4: A multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Mol. Biol. Evol. 27, 221–224 (2010)
Abascal, F., Zardoya, R., Posada, D.: ProtTest: selection of best-fit models of protein evolution. Bioinformatics 21, 2104–2105 (2005)
Guex, N., Peitsch, M.C.: SWISS-MODEL and the Swiss-PdbViewer: An environment for comparative protein modeling (1997)
Benkert, P., Biasini, M., Schwede, T.: Toward the estimation of the absolute quality of individual protein structure models. Bioinformatics 27, 343–350 (2011)
Arnold, K., Bordoli, L., Kopp, J., Schwede, T.: The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling. Bioinformatics 22, 195–201 (2006)
Schwede, T.: SWISS-MODEL: an automated protein homology-modeling server. Nucleic Acids Res. 31, 3381–3385 (2003)
Jimenez-Lopez, J.C., Kotchoni, S.O., Hernandez-Soriano, M.C., Gachomo, E.W., Alché, J.D.: Structural functionality, catalytic mechanism modeling and molecular allergenicity of phenylcoumaran benzylic ether reductase, an olive pollen (Ole e 12) allergen. J. Comput. Aided. Mol. Des. 27, 873–895 (2013)
Hamman-Khalifa, A., Castro, A.J., Jiménez-López, J.C., Rodríguez-García, M.I., Alché, J.D.D.: Olive cultivar origin is a major cause of polymorphism for Ole e 1 pollen allergen. BMC Plant Biol. 8, 10 (2008)
Jimenez-Lopez, J.C., Morales, S., Castro, A.J., Volkmann, D., Rodríguez-García, M.I., Alché, J.D.D.: Characterization of profilin polymorphism in pollen with a focus on multifunctionality. PLoS One 7, e30878 (2012)
Palomares, O., Crameri, R., Rhyner, C.: The contribution of biotechnology toward progress in diagnosis, management, and treatment of allergic diseases. Allergy 69, 1588–1601 (2014)
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer International Publishing Switzerland
About this paper
Cite this paper
Zafra, A., Carmona, R., Jimenez-Lopez, J.C., Pulido, A., Claros, M.G., de Dios Alché, J. (2015). Identification of Distinctive Variants of the Olive Pollen Allergen Ole e 5 (Cu,Zn Superoxide Dismutase) throughout the Analysis of the Olive Pollen Transcriptome. In: Ortuño, F., Rojas, I. (eds) Bioinformatics and Biomedical Engineering. IWBBIO 2015. Lecture Notes in Computer Science(), vol 9043. Springer, Cham. https://doi.org/10.1007/978-3-319-16483-0_45
Download citation
DOI: https://doi.org/10.1007/978-3-319-16483-0_45
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-16482-3
Online ISBN: 978-3-319-16483-0
eBook Packages: Computer ScienceComputer Science (R0)