Conclusions

Abstract

The approaches described here provide rapid and low-cost alternatives for sample preparation, genomic DNA extraction, and mutation discovery. When evaluating the methods, it is important to remember that protocol adaptations may be necessary to compensate for sample differences (species and genotype), environmental conditions in the laboratory, and quality of the water and chemicals used. Cost savings in DNA preparation must be balanced with the shelf-life and suitability of the samples for use in downstream applications. With the appropriate validation of sample quality and longevity, the protocols described here can provide sufficient DNA for a variety of molecular applications such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.

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The approaches described here provide rapid and low-cost alternatives for sample preparation, genomic DNA extraction, and mutation discovery. When evaluating the methods, it is important to remember that protocol adaptations may be necessary to compensate for sample differences (species and genotype), environmental conditions in the laboratory, and quality of the water and chemicals used. Cost savings in DNA preparation must be balanced with the shelf-life and suitability of the samples for use in downstream applications. With the appropriate validation of sample quality and longevity, the protocols described here can provide sufficient DNA for a variety of molecular applications such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.