Authors:
Open Access provided with a grant from the International Atomic Energy Agency
Describes low-cost and easy-to-use assays for the molecular characterization of mutant plant material
The experimental protocols do away with the need for complicated procedures, expensive equipment and hazardous chemicals
All protocols included have been tried and tested by researchers all over the world
Includes supplementary material: sn.pub/extras
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Table of contents (9 chapters)
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Front Matter
About this book
This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
Keywords
- Crop improvement
- DNA extraction
- Induced mutations
- Low-cost assays
- Mutant germplasm characterization
- Nucleotide variation in plant genomes
- PCR amplification
Authors and Affiliations
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Plant Breeding and Genetics Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna, Austria
Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Bernhard J. Hofinger
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Agricultural Research Corporation, Khartoum North, Sudan
Mayada M. Beshir
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School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, USA
Robert G. Laport
Bibliographic Information
Book Title: Low-Cost Methods for Molecular Characterization of Mutant Plants
Book Subtitle: Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols
Authors: Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger
DOI: https://doi.org/10.1007/978-3-319-16259-1
Publisher: Springer Cham
eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)
Copyright Information: The Editor(s) (if applicable) and the Author(s) 2015
License: CC BY-NC
Hardcover ISBN: 978-3-319-16258-4Due: 09 June 2015
Softcover ISBN: 978-3-319-38667-6Published: 17 October 2016
eBook ISBN: 978-3-319-16259-1Published: 08 May 2015
Edition Number: 1
Number of Pages: X, 38
Number of Illustrations: 6 b/w illustrations, 3 illustrations in colour
Topics: Plant Biotechnology, Biological Techniques, Nucleic Acid
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Published in cooperation with
http://link.springer.com/book/10.1007/978-3-319-16259-1
