Abstract
Autonomously replicating vectors are maintained independent of chromosomal replication in fungal cells. In several fungi, autonomously replicating vectors containing the AMA1 sequence derived from Aspergillus nidulans have been used to increase transformation efficiency, improve expression of target genes, and facilitate construction of genomic libraries, suggesting that AMA1-bearing vectors may be applicable to a wide range of fungal species. Here we describe a transformation procedure using an AMA1-bearing vector in the plant pathogenic fungus Rosellinia necatrix. This procedure increases transformation efficiency more than fivefold compared with a genome-integrating vector. The AMA1 vectors were maintained extrachromosomally in most transformants. In addition, co-transformation using multiple AMA1-bearing vectors was also successful. AMA1-bearing vectors are a useful addition to the experimental toolbox for many transformation experiments.
An erratum to this chapter is available at http://dx.doi.org/10.1007/978-3-319-10503-1_22
An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-3-319-10503-1_22
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We appreciate the help of Naoyuki Matsumoto and Hajime Yaegashi for their fruitful comments on the manuscript.
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Kanematsu, S., Shimizu, T. (2015). Transformation of Ascomycetous Fungi Using Autonomously Replicating Vectors. In: van den Berg, M., Maruthachalam, K. (eds) Genetic Transformation Systems in Fungi, Volume 2. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-10503-1_13
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DOI: https://doi.org/10.1007/978-3-319-10503-1_13
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