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Biolistic Transformation of Candida glabrata for Homoplasmic Mitochondrial Genome Transformants

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Genetic Transformation Systems in Fungi, Volume 1

Part of the book series: Fungal Biology ((FUNGBIO))

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Abstract

Genetic operations of mitochondrial genome of non-conventional yeast species is of great interest to investigate physiological functions of those genes located on the genome and many important metabolism functions associated with mitochondria, such as energy metabolism, generation of reactive oxygen species. Here, we describe a method to transform the mitochondrial genome of Candida glabrata by biolistic transformation.

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Acknowledgements

We are thankful to Thomas D. Fox for kind donation of pDS24 and continuous technical support; Nathalie Bonnefoy and Malgorzata Rak for suggestive discussion; and Xiaowei Niu for help with biolistic transformation.

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Authors and Affiliations

  1. School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiansu, 214122, China

    Jingwen Zhou Ph.D., Liming Liu Ph.D., Guocheng Du Ph.D. & Jian Chen Ph.D.

Authors
  1. Jingwen Zhou Ph.D.
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  2. Liming Liu Ph.D.
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  3. Guocheng Du Ph.D.
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  4. Jian Chen Ph.D.
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Corresponding author

Correspondence to Jingwen Zhou Ph.D. .

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Editors and Affiliations

  1. Applied Biochemistry Department, DSM Biotechnology Center, Delft, The Netherlands

    Marco A. van den Berg

  2. Global Marker Technology Lab (DuPont-Pioneer), E.I.DuPont India Pvt Ltd, DuPont Knowledge Center, Hyderabad, Telangana, India

    Karunakaran Maruthachalam

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© 2015 Springer International Publishing Switzerland

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Zhou, J., Liu, L., Du, G., Chen, J. (2015). Biolistic Transformation of Candida glabrata for Homoplasmic Mitochondrial Genome Transformants. In: van den Berg, M., Maruthachalam, K. (eds) Genetic Transformation Systems in Fungi, Volume 1. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-10142-2_11

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