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Genomic Tools for the Study of Azospirillum and Other Plant Growth-Promoting Rhizobacteria

  • Chapter
Handbook for Azospirillum

Abstract

Bioinformatics tools are essential for extracting valuable biological knowledge from bacterial genomes. Currently, there are many computational applications, algorithms, and programs to decipher the genomes in terms of structure, function, and evolution. Specialized databases to upload and retrieve genomic information have grown as well in the last past years. In this chapter, we highlight the basic bioinformatics procedures, databases, and web resources commonly used in bacterial genomics covering Azospirillum and related bacteria.

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References

  • Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS et al (2012) SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 19:455–477

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Benson DA, Clark K, Karsch-Mizrachi I, Lipman DJ, Ostell J, Sayers EW (2014) GenBank. Nucleic Acids Res 42:D32–D37

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Borodovsky M, Lomsadze A (2014) Gene identification in prokaryotic genomes, phages, metagenomes, and EST sequences with GeneMarkS suite. Curr Protoc Microbiol 32:Unit 1E 7

    Google Scholar 

  • Bose M, Barber RD (2006) Prophage Finder: a prophage loci prediction tool for prokaryotic genome sequences. In Silico Biol 6:223–227

    CAS  PubMed  Google Scholar 

  • Butler J, MacCallum I, Kleber M, Shlyakhter IA, Belmonte MK, Lander ES et al (2008) ALLPATHS: de novo assembly of whole-genome shotgun microreads. Genome Res 18:810–820

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Carver T, Harris SR, Berriman M, Parkhill J, McQuillan JA (2012) Artemis: an integrated platform for visualization and analysis of high-throughput sequence-based experimental data. Bioinformatics 28:464–469

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Chaudhuri RR, Loman NJ, Snyder LA, Bailey CM, Stekel DJ, Pallen MJ (2008) xBASE2: a comprehensive resource for comparative bacterial genomics. Nucleic Acids Res 36:D543–D546

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Chen F, Mackey AJ, Stoeckert CJ Jr, Roos DS (2006) OrthoMCL-DB: querying a comprehensive multi-species collection of ortholog groups. Nucleic Acids Res 34:D363–D368

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Chen IM, Markowitz VM, Chu K, Anderson I, Mavromatis K, Kyrpides NC et al (2013) Improving microbial genome annotations in an integrated database context. PLoS One 8:e54859

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Contreras-Moreira B, Vinuesa P (2013) GET_HOMOLOGUES, a versatile software package for scalable and robust microbial pangenome analysis. Appl Environ Microbiol 79:7696–7701

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Darling AC, Mau B, Blattner FR, Perna NT (2004) Mauve: multiple alignment of conserved genomic sequence with rearrangements. Genome Res 14:1394–1403

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Datta RS, Meacham C, Samad B, Neyer C, Sjolander K (2009) Berkeley PHOG: PhyloFacts orthology group prediction web server. Nucleic Acids Res 37:W84–W89

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Delcher AL, Harmon D, Kasif S, White O, Salzberg SL (1999) Improved microbial gene identification with GLIMMER. Nucleic Acids Res 27:4636–4641

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Didelot X, Falush D (2007) Inference of bacterial microevolution using multilocus sequence data. Genetics 175:1251–1266

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Didelot X, Darling A, Falush D (2009) Inferring genomic flux in bacteria. Genome Res 19:306–317

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Didelot X, Lawson D, Darling A, Falush D (2010) Inference of homologous recombination in bacteria using whole-genome sequences. Genetics 186:1435–1449

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Dimmer EC, Huntley RP, Alam-Faruque Y, Sawford T, O’Donovan C, Martin MJ et al (2012) The UniProt-GO Annotation database in 2011. Nucleic Acids Res 40:D565–D570

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Dufayard JF, Duret L, Penel S, Gouy M, Rechenmann F, Perriere G (2005) Tree pattern matching in phylogenetic trees: automatic search for orthologs or paralogs in homologous gene sequence databases. Bioinformatics 21:2596–2603

    Article  CAS  PubMed  Google Scholar 

  • Eid J, Fehr A, Gray J, Luong K, Lyle J, Otto G et al (2009) Real-time DNA sequencing from single polymerase molecules. Science 323:133–138

    Article  CAS  PubMed  Google Scholar 

  • Fonseca NA, Rung J, Brazma A, Marioni JC (2012) Tools for mapping high-throughput sequencing data. Bioinformatics 28:3169–3177

    Article  CAS  PubMed  Google Scholar 

  • Fouts DE (2006) Phage_Finder: automated identification and classification of prophage regions in complete bacterial genome sequences. Nucleic Acids Res 34:5839–5851

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Franceschini A, Szklarczyk D, Frankild S, Kuhn M, Simonovic M, Roth A et al (2013) STRING v9.1: protein-protein interaction networks, with increased coverage and integration. Nucleic Acids Res 41:D808–D815

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Frazer KA, Pachter L, Poliakov A, Rubin EM, Dubchak I (2004) VISTA: computational tools for comparative genomics. Nucleic Acids Res 32:W273–W279

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Gordon D, Green P (2013) Consed: a graphical editor for next-generation sequencing. Bioinformatics 29:2936–2937

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Held K, Ramage E, Jacobs M, Gallagher L, Manoil C (2012) Sequence-verified two-allele transposon mutant library for Pseudomonas aeruginosa PAO1. J Bacteriol 194:6387–6389

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Huerta-Cepas J, Bueno A, Dopazo J, Gabaldon T (2008) PhylomeDB: a database for genome-wide collections of gene phylogenies. Nucleic Acids Res 36:D491–D496

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Hunter S, Jones P, Mitchell A, Apweiler R, Attwood TK, Bateman A et al (2012) InterPro in 2011: new developments in the family and domain prediction database. Nucleic Acids Res 40:D306–D312

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Hyatt D, Chen GL, Locascio PF, Land ML, Larimer FW, Hauser LJ (2010) Prodigal: prokaryotic gene recognition and translation initiation site identification. BMC Bioinformatics 11:19

    Google Scholar 

  • Jeck WR, Reinhardt JA, Baltrus DA, Hickenbotham MT, Magrini V, Mardis ER et al (2007) Extending assembly of short DNA sequences to handle error. Bioinformatics 23:2942–2944

    Article  CAS  PubMed  Google Scholar 

  • Jones P, Binns D, Chang HY, Fraser M, Li W, McAnulla C et al (2014) InterProScan 5: genome-scale protein function classification. Bioinformatics 30:1236–1240

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Kanehisa M, Goto S, Sato Y, Furumichi M, Tanabe M (2012) KEGG for integration and interpretation of large-scale molecular data sets. Nucleic Acids Res 40:D109–D114

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Kaneko T, Minamisawa K, Isawa T, Nakatsukasa H, Mitsui H, Kawaharada Y et al (2010) Complete genomic structure of the cultivated rice endophyte Azospirillum sp. B510. DNA Res 17:37–50

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Koonin EV, Wolf YI (2008) Genomics of bacteria and archaea: the emerging dynamic view of the prokaryotic world. Nucleic Acids Res 36:6688–6719

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Koren S, Treangen TJ, Hill CM, Pop M, Phillippy AM (2014) Automated ensemble assembly and validation of microbial genomes. BMC Bioinformatics 15:126

    Article  PubMed Central  PubMed  Google Scholar 

  • Kosuge T, Mashima J, Kodama Y, Fujisawa T, Kaminuma E, Ogasawara O et al (2014) DDBJ progress report: a new submission system for leading to a correct annotation. Nucleic Acids Res 42:D44–D49

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Kristensen DM, Cai X, Mushegian A (2011a) Evolutionarily conserved orthologous families in phages are relatively rare in their prokaryotic hosts. J Bacteriol 193:1806–1814

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Kristensen DM, Wolf YI, Mushegian AR, Koonin EV (2011b) Computational methods for Gene Orthology inference. Brief Bioinform 12:379–391

    Article  PubMed Central  PubMed  Google Scholar 

  • Kulikova T, Akhtar R, Aldebert P, Althorpe N, Andersson M, Baldwin A et al (2007) EMBL nucleotide sequence database in 2006. Nucleic Acids Res 35:D16–D20

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Kurtz S, Phillippy A, Delcher AL, Smoot M, Shumway M, Antonescu C et al (2004) Versatile and open software for comparing large genomes. Genome Biol 5:R12

    Article  PubMed Central  PubMed  Google Scholar 

  • Kuzniar A, van Ham RC, Pongor S, Leunissen JA (2008) The quest for orthologs: finding the corresponding gene across genomes. Trends Genet 24:539–551

    Article  CAS  PubMed  Google Scholar 

  • Langille MG, Brinkman FS (2009) IslandViewer: an integrated interface for computational identification and visualization of genomic islands. Bioinformatics 25:664–665

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Langmead B, Trapnell C, Pop M, Salzberg SL (2009) Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol 10:R25

    Article  PubMed Central  PubMed  Google Scholar 

  • Lee H, Tang H (2012) Next-generation sequencing technologies and fragment assembly algorithms. Methods Mol Biol 855:155–174

    Article  CAS  PubMed  Google Scholar 

  • Li L, Stoeckert CJ Jr, Roos DS (2003) OrthoMCL: identification of ortholog groups for eukaryotic genomes. Genome Res 13:2178–2189

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Li R, Yu C, Li Y, Lam TW, Yiu SM, Kristiansen K et al (2009) SOAP2: an improved ultrafast tool for short read alignment. Bioinformatics 25:1966–1967

    Article  CAS  PubMed  Google Scholar 

  • Lima-Mendez G, Van Helden J, Toussaint A, Leplae R (2008) Prophinder: a computational tool for prophage prediction in prokaryotic genomes. Bioinformatics 24:863–865

    Article  CAS  PubMed  Google Scholar 

  • Loman NJ, Misra RV, Dallman TJ, Constantinidou C, Gharbia SE, Wain J et al (2012) Performance comparison of benchtop high-throughput sequencing platforms. Nat Biotechnol 30:434–439

    Article  CAS  PubMed  Google Scholar 

  • Lozano L, Hernandez-Gonzalez I, Bustos P, Santamaria RI, Souza V, Young JP et al (2010) Evolutionary dynamics of insertion sequences in relation to the evolutionary histories of the chromosome and symbiotic plasmid genes of Rhizobium etli populations. Appl Environ Microbiol 76:6504–6513

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Magoc T, Pabinger S, Canzar S, Liu X, Su Q, Puiu D et al (2013) GAGE-B: an evaluation of genome assemblers for bacterial organisms. Bioinformatics 29:1718–1725

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Magrane M, Consortium U (2011) UniProt Knowledgebase: a hub of integrated protein data. Database (Oxford) 2011:bar009

    Article  Google Scholar 

  • Mardis ER (2011) A decade’s perspective on DNA sequencing technology. Nature 470:198–203

    Article  CAS  PubMed  Google Scholar 

  • Mardis ER (2013) Next-generation sequencing platforms. Annu Rev Anal Chem (Palo Alto Calif) 6:287–303

    Article  CAS  Google Scholar 

  • Margulies M, Egholm M, Altman WE, Attiya S, Bader JS, Bemben LA et al (2005) Genome sequencing in microfabricated high-density picolitre reactors. Nature 437:376–380

    PubMed Central  CAS  PubMed  Google Scholar 

  • Markowitz VM, Chen IM, Palaniappan K, Chu K, Szeto E, Pillay M et al (2014) IMG 4 version of the integrated microbial genomes comparative analysis system. Nucleic Acids Res 42:D560–D567

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Mi H, Guo N, Kejariwal A, Thomas PD (2007) PANTHER version 6: protein sequence and function evolution data with expanded representation of biological pathways. Nucleic Acids Res 35:D247–D252

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Miller JR, Koren S, Sutton G (2010) Assembly algorithms for next-generation sequencing data. Genomics 95:315–327

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Moriya Y, Itoh M, Okuda S, Yoshizawa AC, Kanehisa M (2007) KAAS: an automatic genome annotation and pathway reconstruction server. Nucleic Acids Res 35:W182–W185

    Article  PubMed Central  PubMed  Google Scholar 

  • Nielsen CB, Cantor M, Dubchak I, Gordon D, Wang T (2010) Visualizing genomes: techniques and challenges. Nat Methods 7:S5–S15

    Article  CAS  PubMed  Google Scholar 

  • Ostlund G, Schmitt T, Forslund K, Kostler T, Messina DN, Roopra S et al (2010) InParanoid 7: new algorithms and tools for eukaryotic orthology analysis. Nucleic Acids Res 38: D196–D203

    Article  PubMed Central  PubMed  Google Scholar 

  • Overbeek R, Olson R, Pusch GD, Olsen GJ, Davis JJ, Disz T et al (2014) The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST). Nucleic Acids Res 42:D206–D214

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Pop M, Kosack D (2004) Using the TIGR assembler in shotgun sequencing projects. Methods Mol Biol 255:279–294

    CAS  PubMed  Google Scholar 

  • Powell S, Forslund K, Szklarczyk D, Trachana K, Roth A, Huerta-Cepas J et al (2014) eggNOG v4.0: nested orthology inference across 3686 organisms. Nucleic Acids Res 42:D231–D239

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Pruitt KD, Tatusova T, Brown GR, Maglott DR (2012) NCBI Reference Sequences (RefSeq): current status, new features and genome annotation policy. Nucleic Acids Res 40:D130–D135

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Ren X, Liu T, Dong J, Sun L, Yang J, Zhu Y et al (2012) Evaluating de Bruijn graph assemblers on 454 transcriptomic data. PLoS One 7:e51188

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Ribeiro FJ, Przybylski D, Yin S, Sharpe T, Gnerre S, Abouelleil A et al (2012) Finished bacterial genomes from shotgun sequence data. Genome Res 22:2270–2277

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Rivera D, Revale S, Molina R, Gualpa J, Puente M, Maroniche G et al (2014) Complete genome sequence of the model rhizosphere strain Azospirillum brasilense Az39, successfully applied in agriculture. Genome Announc 2(4), pii: e00683-14

    Google Scholar 

  • Royce L, Boggess E, Jin T, Dickerson J, Jarboe L (2013) Identification of mutations in evolved bacterial genomes. Methods Mol Biol 985:249–267

    Article  CAS  PubMed  Google Scholar 

  • Santamaria RI, Bustos P, Sepulveda-Robles O, Lozano L, Rodriguez C, Fernandez JL et al (2014) Narrow-host-range bacteriophages that infect Rhizobium etli associate with distinct genomic types. Appl Environ Microbiol 80:446–454

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Schneider GF, Dekker C (2012) DNA sequencing with nanopores. Nat Biotechnol 30:326–328

    Article  CAS  PubMed  Google Scholar 

  • Siguier P, Perochon J, Lestrade L, Mahillon J, Chandler M (2006) ISfinder: the reference centre for bacterial insertion sequences. Nucleic Acids Res 34:D32–D36

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Simpson JT, Wong K, Jackman SD, Schein JE, Jones SJ, Birol I (2009) ABySS: a parallel assembler for short read sequence data. Genome Res 19:1117–1123

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Tatusov RL, Fedorova ND, Jackson JD, Jacobs AR, Kiryutin B, Koonin EV et al (2003) The COG database: an updated version includes eukaryotes. BMC Bioinformatics 4:41

    Article  PubMed Central  PubMed  Google Scholar 

  • UniPort Consortium (2014) Activities at the Universal Protein Resource (UniProt). Nucleic Acids Res 42:D191–D198

    Article  Google Scholar 

  • Vacheron J, Desbrosses G, Bouffaud ML, Touraine B, Moenne-Loccoz Y, Muller D et al (2013) Plant growth-promoting rhizobacteria and root system functioning. Front Plant Sci 4:356

    Article  PubMed Central  PubMed  Google Scholar 

  • Vallenet D, Belda E, Calteau A, Cruveiller S, Engelen S, Lajus A et al (2013) MicroScope–an integrated microbial resource for the curation and comparative analysis of genomic and metabolic data. Nucleic Acids Res 41:D636–D647

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Vilella AJ, Severin J, Ureta-Vidal A, Heng L, Durbin R, Birney E (2009) EnsemblCompara GeneTrees: Complete, duplication-aware phylogenetic trees in vertebrates. Genome Res 19(2):327–35

    Google Scholar 

  • Wagner A, Lewis C, Bichsel M (2007) A survey of bacterial insertion sequences using IScan. Nucleic Acids Res 35:5284–5293

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Waterhouse RM, Tegenfeldt F, Li J, Zdobnov EM, Kriventseva EV (2013) OrthoDB: a hierarchical catalog of animal, fungal and bacterial orthologs. Nucleic Acids Res 41:D358–D365

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Wisniewski-Dye F, Borziak K, Khalsa-Moyers G, Alexandre G, Sukharnikov LO, Wuichet K et al (2011) Azospirillum genomes reveal transition of bacteria from aquatic to terrestrial environments. PLoS Genet 7:e1002430

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Wisniewski-Dye F, Lozano L, Acosta-Cruz E, Borland S, Drogue B, Prigent-Combaret C et al (2012) Genome sequence of Azospirillum brasilense CBG497 and comparative analyses of Azospirillum core and accessory genomes provide insight into niche adaptation. Genes (Basel) 3:576–602

    Article  CAS  Google Scholar 

  • Yu C, Desai V, Cheng L, Reifman J (2012) QuartetS-DB: a large-scale orthology database for prokaryotes and eukaryotes inferred by evolutionary evidence. BMC Bioinformatics 13:143

    Article  PubMed Central  PubMed  Google Scholar 

  • Zerbino DR, Birney E (2008) Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res 18:821–829

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Zhao Y, Wu J, Yang J, Sun S, Xiao J, Yu J (2012) PGAP: pan-genomes analysis pipeline. Bioinformatics 28:416–418

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Zhao Y, Jia X, Yang J, Ling Y, Zhang Z, Yu J et al (2014) PanGP: a tool for quickly analyzing bacterial pan-genome profile. Bioinformatics 30:1297–1299

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Zhou Y, Liang Y, Lynch KH, Dennis JJ, Wishart DS (2011) PHAST: a fast phage search tool. Nucleic Acids Res 39:W347–W352

    Article  PubMed Central  CAS  PubMed  Google Scholar 

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Acknowledgments

The work on bacterial genomics is supported by grants to VG from CONACYT CB 131499 and PAPPIT-UNAM IN2084143. We wish to thank to Olga M. Carrascal and Irma Martínez for valuable comments to the manuscript and José Espíritu for computational help.

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Authors and Affiliations

  1. Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Avenida Universidad N/C Col. Chamilpa Apdo. Postal 565-A, Cuernavaca, Morelos, C. P. 62300, Mexico

    Víctor González, Luis Lozano, Patricia Bustos & Rosa I. Santamaría

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  1. Víctor González
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  2. Luis Lozano
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  3. Patricia Bustos
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  4. Rosa I. Santamaría
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Correspondence to Víctor González .

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Editors and Affiliations

  1. Laboratorio de Fisiología Vegetal y de la Interacción Planta-Microorganismo, Universidad Nacional de Rio Cuarto, Rio Cuarto, Córdoba, Argentina

    Fabricio Dario Cassán

  2. Faculty of Agricultue Food and Environme, The Hebrew University of Jerusalem, Rehovot, Central, Israel

    Yaacov Okon

  3. Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, Balcarce, Argentina

    Cecilia M. Creus

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González, V., Lozano, L., Bustos, P., Santamaría, R.I. (2015). Genomic Tools for the Study of Azospirillum and Other Plant Growth-Promoting Rhizobacteria. In: Cassán, F., Okon, Y., Creus, C. (eds) Handbook for Azospirillum. Springer, Cham. https://doi.org/10.1007/978-3-319-06542-7_5

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