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Structure and Expression of the Human Metallothionein Genes

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Metallothionein II

Part of the book series: Experientia Supplementum ((EXS,volume 52))

Abstract

The human metallothioneins are represented by a multigene family consisting of about 14 members. A number of MT-like genes have been isolated from a human genomic library and in this report, four MT genes have been characterized. Our results show that two of these genes represent the MT-I and MT-II processed genes. The other two genes (MT-IF and MT-IG) are functional members of the MT-I gene family. The amino acid sequence encoded by the MT-IF and MT-IG genes differ from the amino acid sequences of the published MT-I proteins at few positions. The 5′-flanking region of these genes contain metal responsive elements. Our studies show that the MT-IF and MT-IG genes are differentially regulated in two human hepatoma cell lines, HepG2 and Hep3B2, and a human lymphoblastoid cell line, WI-L2 in response to the heavy metals cadmium, zinc and copper, and glucocorticoids. In addition, these genes also show cell-type specific expression.

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Author information

Authors and Affiliations

  1. Department of Biology and the University Biochemistry Group, University of Calgary, Calgary, Alberta, T2N 1N4, Canada

    Dr. Lashitew Gedamu, U. Varshney, N. Jahroudi, R. Foster & N. W. Shworak

Authors
  1. Dr. Lashitew Gedamu
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  2. U. Varshney
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  3. N. Jahroudi
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  4. R. Foster
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  5. N. W. Shworak
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Editor information

Editors and Affiliations

  1. Institute of Biochemistry, University of Zürich, Winterthurerstrasse 190, CH-8057, Zürich, Switzerland

    Jeremias H. R. Kägi

  2. Dept. of Environmental Medicine, Hokkaido University, Kita-ku, Sapporo, 060, Japan

    Yutaka Kojima

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© 1987 Springer Basel AG

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Gedamu, L., Varshney, U., Jahroudi, N., Foster, R., Shworak, N.W. (1987). Structure and Expression of the Human Metallothionein Genes. In: Kägi, J.H.R., Kojima, Y. (eds) Metallothionein II. Experientia Supplementum, vol 52. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-6784-9_34

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  • DOI: https://doi.org/10.1007/978-3-0348-6784-9_34

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-0348-6786-3

  • Online ISBN: 978-3-0348-6784-9

  • eBook Packages: Springer Book Archive

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