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Current Techniques for Intraoperative Application

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Intraoperative Flow Cytometry

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Abstract

Complete surgical resection with clear margins remains the basis of therapy for patients with head and neck squamous cell carcinoma. Intraoperative frozen section analysis of surgical margins remains the most common and standard technique for the assessment of surgical margins. Although in the hands of experienced pathologists frozen section diagnoses tend to have high accuracy, discrepancies between frozen section analysis and permanent histopathology still occur. Intraoperative margin analysis with different imaging techniques have gained a lot of interest. Optical imaging techniques in the head and neck include: autofluorescence imaging (AFI), targeted fluorescence imaging (TFI), high-resolution microendoscopy (HRME), narrow band imaging (NBI), and the Raman spectroscopy (RS). Optical coherence tomography, elastic scattering spectroscopy, confocal laser endomicroscopy, and confocal reflectance microscopy, have also been used in the head and neck region.

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Authors and Affiliations

  1. Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital of Ioannina, Ioannina, Greece

    Ioannis Kastanioudakis & Lentiona Basiari

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  1. Ioannis Kastanioudakis
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  2. Lentiona Basiari
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Editors and Affiliations

  1. Dept. of Neurosurgery, University of Ioannina, Ioannina, Greece

    Georgios Alexiou

  2. Unit of Molecular Biology and Translational Flow Cytometry, University Hospital of Ioannina, Ioannina, Greece

    Georgios Vartholomatos

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Kastanioudakis, I., Basiari, L. (2023). Current Techniques for Intraoperative Application. In: Alexiou, G., Vartholomatos, G. (eds) Intraoperative Flow Cytometry. Springer, Cham. https://doi.org/10.1007/978-3-031-33517-4_16

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  • DOI: https://doi.org/10.1007/978-3-031-33517-4_16

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