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Capture and Concentration of Circulating Cancer-Associated Extracellular Vesicles

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Circulating Tumor Cells

Part of the book series: Current Cancer Research ((CUCR))

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Abstract

Extracellular vesicles (EVs) are a category of nanoscale membrane-bound bodies secreted by nearly every cell in the body and found in nearly all bodily fluids. EVs have been shown to contain diverse molecular cargos, including proteins, RNA, lipid, and DNA. As EVs are derived from their parental cells and those EV molecular cargos are uniquely protected from the extracellular environment by EV membrane, EVs provides high quality of potential molecular biomarkers for cancer diagnosis. However, the heterogeneity of EV populations and lack of highly specific surface cancer marker on EV membrane pose significant challenges in separating tumor-derived EVs (tdEV) from high background of healthy cell-derived EVs in peripheral blood. Here we discussed the recent technology advancements in the field of enriching subpopulations of EVs from bulk populations, with a focus on tdEV enrichment for cancer liquid biopsy purposes.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA

    Jeongyun Kim, Jacob Rast, Hong-Zhang He & Si-Yang Zheng

  2. Captis Diagnostics Inc, Pittsburgh, PA, USA

    Hong-Zhang He & Si-Yang Zheng

  3. Electrical & Computer Engineering, Carnegie Mellon University, Pittsburgh, PA, USA

    Si-Yang Zheng

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  1. Jeongyun Kim
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  2. Jacob Rast
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  3. Hong-Zhang He
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  4. Si-Yang Zheng
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Correspondence to Hong-Zhang He or Si-Yang Zheng .

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Editors and Affiliations

  1. Department of Pathology and Immunology, Washington University in St. Louis School of Medicine, St. Louis, MO, USA

    Richard J. Cote

  2. ACTC Lab, Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Athens, Greece

    Evi Lianidou

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Kim, J., Rast, J., He, HZ., Zheng, SY. (2023). Capture and Concentration of Circulating Cancer-Associated Extracellular Vesicles. In: Cote, R.J., Lianidou, E. (eds) Circulating Tumor Cells. Current Cancer Research. Springer, Cham. https://doi.org/10.1007/978-3-031-22903-9_5

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