Abstract
The depletion of fossil fuels has increased demand for alternative energy resources globally. Currently, bioethanol and biodiesel produced via first and second generation technologies are the most attractive biofuels, which have shown sustainability as renewable energy sources. The challenge with first and second generation biofuels is that feedstocks are associated with food security and there is lower yield of the process. Recently, third generation bioethanol from microalgae and macroalgae has been shown to be an emerging technology for the biofuel industry globally. The advantage is that this system does not require large amounts of land and pure water. Moreover, bioethanol that has been produced from algae has been shown to have higher yield compared to the second generation production process. Therefore, the main aim of this review is to take a detailed look at the third generation bioethanol technologies and the prospective future of the process. The pretreatment processes that are associated with processing microalgae and macroalgae to generate fermentable sugars for bioethanol production are also discussed.
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References
Abdul Latif NIS, Ong MY, Nomanbhay S (2019) Hydrothermal liquefaction of Malaysia's algal biomass for high-quality bio-oil production. Eng Life Sci 19:246–269
Ahmed N, Dhar BR, Pramanik BK, Forehead H, Price WE, Hai FI (2021) A cookbook for bioethanol from macroalgae: review of selecting and combining processes to enhance bioethanol production. Curr Pollution Rep. https://doi.org/10.1007/s40726-021-00202-7
Al Abdallah Q, Nixon BT, Fortwendel JR (2016) The enzymatic conversion of major algal and cyanobacterial carbohydrates to bioethanol. Front Energ Res 4:1–15
Alam MA, Yuan T, Xiong W, Zhang B, Lv Y, Xu J (2019) Process optimization for the production of high-concentration ethanol with Scenedesmus raciborskii biomass. Bioresour Technol 294:122219
Aparicio E, RodrÃguez-Jasso RM, Pinales-Márquez CD, Loredo-Treviño A, Robledo-Olivo A, Aguilar CN, Kostas ET, Ruiz HA (2021) High-pressure technology for Sargassum spp biomass pretreatment and fractionation in the third generation of bioethanol production. Bioresour Technol 329:1–10
Bader AN, Rizza LS, Consolo VF, Curatti L (2020) Efficient saccharification of microalgal biomass by Trichoderma harzianum enzymes for the production of ethanol. Algal Res 48:1–9
Balina K, Lika A, Romagnoli F, Blumberga D (2017) Seaweed cultivation laboratory testing: effects of nutrients on growth rate of Ulva intestinalis. Energy Procedia 113:454–459
Bedzo OK, Dreyer CB, van Rensburg E, Görgens JF (2021) Optimisation of pretreatment catalyst, enzyme cocktail and solid loading for improved ethanol production from sweet sorghum bagasse. Bioenergy Res. https://doi.org/10.1007/s12155-021-10298-w
Bhatia L, Bachheti RK, Garlapati VK, Chandel AK (2020) Third-generation biorefineries: a sustainable platform for food, clean energy, and nutraceuticals production. Biomass Convers Biorefin. https://doi.org/10.1007/s13399-020-00843-6
Bhore N (2014) Energy outlook: a view to 2040. Detroit Automotive Petroleum Forum, Detroit, MI, USA, pp 1–35
Bold HC (1978) Introduction to the algae. Prentice-Hall, Hoboken
Cardona CA, Sánchez ÓJ (2007) Fuel ethanol production: process design trends and integration opportunities. Bioresour Technol 98:2415–2457
Carriquiry MA, Du X, Timilsina GR (2011) Second generation biofuels: economics and policies. Energy Policy 39:4222–4234
Cho H, Ra C-H, Kim S-K (2014) Ethanol production from the seaweed Gelidium amansii, using specific sugar acclimated yeasts. J Microbiol Biotechnol 24:264–269
Choi W, Han J, Lee C, Song C, Kim J, Seo Y, Lee S, Jung K, Kang D, Heo S (2012) Bioethanol production from Ulva pertusa Kjellman by high-temperature liquefaction. Chem Biochem Eng Q 26:15–21
Choi YY, Patel AK, Hong ME, Chang WS, Sim SJ (2019) Microalgae bioenergy with carbon capture and storage (BECCS): an emerging sustainable bioprocess for reduced CO2 emission and biofuel production. Bioresour Technol Rep 7:100256–100270
Chong TY, Cheah SA, Ong CT, Wong LY, Goh CR, Tan IS, Foo HCY, Lam MK, Lim S (2020) Techno-economic evaluation of third-generation bioethanol production utilizing the macroalgae waste: a case study in Malaysia. Energy 210:118483–118491
Chung MRWY, Tan IS, Foo HCY, Lam MK, Lim S (2021) Potential of macroalgae-based biorefinery for lactic acid production from exergy aspect. Biomass Convers Biorefin. https://doi.org/10.1007/s13399-021-01375-3
Cui T, Yuan B, Guo H, Tian H, Wang W, Ma Y, Li C, Fei Q (2021) Enhanced lignin biodegradation by consortium of white rot fungi: microbial synergistic effects and product mapping. Biotechnol Biofuels 14:162
Dalla Marta A, Mancini M, Orlando F, Natali F, Capecchi L, Orlandini S (2014) Sweet sorghum for bioethanol production: crop responses to different water stress levels. Biomass Bioenergy 64:211–219
Dave N, Selvaraj R, Varadavenkatesan T, Vinayagam R (2019) A critical review on production of bioethanol from macroalgal biomass. Algal Res 42:101606
del RÃo PG, Gullón B, Pérez-Pérez A, Romanà A, Garrote G (2021) Microwave hydrothermal processing of the invasive macroalgae Sargassum muticum within a green biorefinery scheme. Bioresour Technol 340:1–10
Edeh I (2020) Bioethanol production: an overview. In: Inambao F (ed) Bioethanol technologies. IntechOpen, pp 1–22
Ekborg NA, Taylor LE, Longmire AG, Henrissat B, Weiner RM, Hutcheson SW (2006) Genomic and proteomic analyses of the agarolytic system expressed by Saccharophagus degradans 2-40. Appl Environ Microbiol 72:3396–3405
GarcÃa-López D, OlguÃn E, González-Portela R, Sánchez-Galván G, De Philippis R, Lovitt R, Llewellyn C, Fuentes-Grünewald C, SaldÃvar RP (2020) A novel two-phase bioprocess for the production of Arthrospira (spirulina) maxima LJGR1 at pilot plant scale during different seasons and for phycocyanin induction under controlled conditions. Bioresour Technol 298:1–11
Gohain M, Hasin M, Eldiehy KS, Bardhan P, Laskar K, Phukon H, Mandal M, Kalita D, Deka D (2021) Bio-ethanol production: a route to sustainability of fuels using bio-based heterogeneous catalyst derived from waste. Process Saf Environment Prot 146:190–200
Greetham D, Adams JM, Du C (2020) The utilization of seawater for the hydrolysis of macroalgae and subsequent bioethanol fermentation. Sci Rep 10:1–15
Gupta PL, Lee S-M, Choi H-J (2015) A mini review: photobioreactors for large scale algal cultivation. World J Microbiol Biotechnol 31:1409–1417
Hafting JT, Craigie JS, Stengel DB, Loureiro RR, Buschmann AH, Yarish C, Edwards MD, Critchley AT (2015) Prospects and challenges for industrial production of seaweed bioactives. J Phycol 51:821–837
Ho S-H, Huang S-W, Chen C-Y, Hasunuma T, Kondo A, Chang J-S (2013) Bioethanol production using carbohydrate-rich microalgae biomass as feedstock. Bioresour Technol 135:191–198
Hong IK, Jeon H, Lee SB (2014) Comparison of red, brown and green seaweeds on enzymatic saccharification process. J Ind Eng Chem 20:2687–2691
Hong Y, Wu Y-R (2020) Acidolysis as a biorefinery approach to producing advanced bioenergy from macroalgal biomass: a state-of-the-art review. Bioresour Technol 318:124080
Huang X, Bai S, Liu Z, Hasunuma T, Kondo A, Ho S-H (2020) Fermentation of pigment-extracted microalgal residue using yeast cell-surface display: direct high-density ethanol production with competitive life cycle impacts. Green Chem 22:153–162
Khalil SRA, Abdelhafez AA, Amer EAM (2015) Evaluation of bioethanol production from juice and bagasse of some sweet sorghum varieties. Ann Agric Sci 60:317–324
Khan MI, Shin JH, Kim JD (2018) The promising future of microalgae: current status, challenges, and optimization of a sustainable and renewable industry for biofuels, feed, and other products. Microb Cell Factories 17:36
Kim D-H, Lee S-B, Jeong G-T (2014) Production of reducing sugar from Enteromorpha intestinalis by hydrothermal and enzymatic hydrolysis. Bioresour Technol 161:348–353
Kim JK, Yarish C, Hwang EK, Park M, Kim Y (2017) Seaweed aquaculture: cultivation technologies, challenges and its ecosystem services. Algae 32:1–13
Kostas ET, White DA, Cook DJ (2020) Bioethanol production from UK seaweeds: investigating variable pre-treatment and enzyme hydrolysis parameters. Bioenergy Res 13:271–285
Kostas ET, White DA, Du C, Cook DJ (2016) Selection of yeast strains for bioethanol production from UK seaweeds. J Appl Phycol 28:1427–1441
Kumar BR, Mathimani T, Sudhakar MP, Rajendran K, Nizami A-S, Brindhadevi K, Pugazhendhi A (2021) A state of the art review on the cultivation of algae for energy and other valuable products: application, challenges, and opportunities. Renew Sust Energ Rev 138:110620–110649
Kumar K, Mishra SK, Shrivastav A, Park MS, Yang J-W (2015) Recent trends in the mass cultivation of algae in raceway ponds. Renew Sust Energ Rev 51:875–885
Kumar AK, Sharma S (2017) Recent updates on different methods of pretreatment of lignocellulosic feedstocks: a review. Bioresour and Bioprocess 4:1–19
Kumar M, Sun Y, Rathour R, Pandey A, Thakur IS, Tsang DC (2020) Algae as potential feedstock for the production of biofuels and value-added products: opportunities and challenges. Sci Total Environ 716:1–17
Laopaiboon L, Thanonkeo P, Jaisil P, Laopaiboon P (2007) Ethanol production from sweet sorghum juice in batch and fed-batch fermentations by Saccharomyces cerevisiae. World J Microbiol Biotechnol 23:1497–1501
Lee SY, Chang JH, Lee SB (2014) Chemical composition, saccharification yield, and the potential of the green seaweed Ulva pertusa. Biotechnol Bioprocess Eng 19:1022–1033
Liang S, Wei L, Passero ML, Feris K, McDonald AG (2017) Hydrothermal liquefaction of laboratory cultivated and commercial algal biomass into crude bio-oil. Environ Prog Sustain Energy 36:781–787
Macedo AA, Medeiros RG, Silvério TAB, Nelson DL, Oliveira DCS, dos Reis AB (2020) Possibilities and prospects regarding ethanol production from saccharin sorghum [Sorghum bicolor (L.) Moench]. SN Appl Sci 2:1–12
Marquez GPB, Santiañez WJE, Trono GC Jr, Montaño MNE, Araki H, Takeuchi H, Hasegawa T (2014) Seaweed biomass of the Philippines: sustainable feedstock for biogas production. Renew Sustain Energ Rev 38:1056–1068
Matanjun P, Mohamed S, Mustapha NM, Muhammad K (2009) Nutrient content of tropical edible seaweeds, Eucheuma cottonii, Caulerpa lentillifera and Sargassum polycystum. J Appl Phycol 21:75–80
Menegazzo ML, Fonseca GG (2019) Biomass recovery and lipid extraction processes for microalgae biofuels production: a review. Renew Sustain Energ Rev 107:87–107
Moser BR (2010) Camelina (Camelina sativa L.) oil as a biofuels feedstock: Golden opportunity or false hope? Lipid Technol 22:270–273
Mussatto SI, Dragone G, Guimarães PM, Silva JPA, Carneiro LM, Roberto IC, Vicente A, Domingues L, Teixeira JA (2010) Technological trends, global market, and challenges of bio-ethanol production. Biotechnol Adv 28:817–830
Omoni VT, Lag-Brotons AJ, Ibeto CN, Semple KT (2021) Effects of biological pre-treatment of lignocellulosic waste with white-rot fungi on the stimulation of 14C-phenanthrene catabolism in soils. Int Biodeterior Biodegradation 165:105324
Oswald WJ, Golueke CG (1960) Biological transformation of solar energy. Adv Appl Microbiol 2:223–262
Pablo G, Gomes-Dias JS, Rocha CM, Romanà A, Garrote G, Domingues L (2020) Recent trends on seaweed fractionation for liquid biofuels production. Bioresour Technol 299:1–15
Panahi HKS, Dehhaghi M, Aghbashlo M, Karimi K, Tabatabaei M (2019) Shifting fuel feedstock from oil wells to sea: Iran outlook and potential for biofuel production from brown macroalgae (ochrophyta; phaeophyceae). Renew Sust Energ Rev 112:626–642
Pang N, Gu X, Chen S, Kirchhoff H, Lei H, Roje S (2019) Exploiting mixotrophy for improving productivities of biomass and co-products of microalgae. Renew Sustain Energ Rev 112:450–460
Peñuela A, Robledo D, Bourgougnon N, Bedoux G, Hernández-Núñez E, Freile-PelegrÃn Y (2018) Environmentally friendly valorization of Solieria filiformis (Gigartinales, Rhodophyta) from IMTA using a biorefinery concept. Mar Drugs 16:1–19
Pereira H, Silva J, Santos T, Gangadhar KN, Raposo A, Nunes C, Coimbra MA, Gouveia L, Barreira L, Varela J (2019) Nutritional potential and toxicological evaluation of Tetraselmis sp. CTP4 microalgal biomass produced in industrial photobioreactors. Molecules 24:1–18
Qarri A, Israel A (2020) Seasonal biomass production, fermentable saccharification and potential ethanol yields in the marine macroalga Ulva sp.(Chlorophyta). Renew Energ 145:2101–2107
Rajak RC, Jacob S, Kim BS (2020) A holistic zero waste biorefinery approach for macroalgal biomass utilization: a review. Sci Total Environ 716:1–17
Ramachandra TV, Hebbale D (2020) Bioethanol from macroalgae: prospects and challenges. Renew Sustain Energ Rev 117:1–18
Salvi KP, da Silva OW, Horta PA, Rörig LR, de Oliveira BE (2021) A new model of algal turf scrubber for bioremediation and biomass production using seaweed aquaculture principles. J Appl Phycol. https://doi.org/10.1007/s10811-021-02430-2
Santos SCR, Ungureanu G, Volf I, Boaventura RAR, Botelho CMS (2018) Macroalgae biomass as sorbent for metal ions. In: Popa V, Volf I (eds) Biomass as renewable raw material to obtain bioproducts of high-tech value. Elsevier, Amsterdam, pp 69–112
Satari B, Jaiswal AK (2021) Green fractionation of 2G and 3G feedstocks for ethanol production: advances, incentives and barriers. Curr Opin Food Sci 37:1–9
Schultz-Jensen N, Thygesen A, Leipold F, Thomsen ST, Roslander C, Lilholt H, Bjerre AB (2013) Pretreatment of the macroalgae Chaetomorpha linum for the production of bioethanol–comparison of five pretreatment technologies. Bioresour Technol 140:36–42
Seo G, Kim HS, Cho JM, Kim M, Park W-K, Chang YK (2020) Effect of post-treatment process of microalgal hydrolysate on bioethanol production. Sci Rep 10:1–12
Sharma B, Larroche C, Dussap C-G (2020) Comprehensive assessment of 2G bioethanol production. Bioresour Technol 313:123630
Smachetti MES, Rizza LS, Coronel CD, Do Nascimento M, Curatti L (2018) Microalgal biomass as an alternative source of sugars for the production of bioethanol. In: Kulia A, Sharma V (eds) Principles and applications of fermentation technology. John Wiley & Sons, Hoboken, pp 351–386
Sun Z, Liu J, Zhou Z-G (2016) Algae for biofuels: an emerging feedstock. In: Luque R, Lin CSK, Wilson K, Clark J (eds) Handbook of biofuels production, 2nd edn. Woodhead Publishing, Cambridge, pp 673–698
Tan IS, Lam MK, Foo HCY, Lim S, Lee KT (2020) Advances of macroalgae biomass for the third generation of bioethanol production. Chin J Chem Eng 28:502–517
Tapia-Tussell R, Avila-Arias J, DomÃnguez Maldonado J, Valero D, Olguin-Maciel E, Pérez-Brito D, Alzate-Gaviria L (2018) Biological pretreatment of mexican caribbean macroalgae consortiums using Bm-2 strain (Trametes hirsuta) and its enzymatic broth to improve biomethane potential. Energies 11:1–11
Tedesco S, Benyounis K, Olabi A (2013) Mechanical pretreatment effects on macroalgae-derived biogas production in co-digestion with sludge in Ireland. Energy 61:27–33
Teixeira ACR, Sodré JR, Guarieiro LLN, Vieira ED, De Medeiros FF, Alves CT (2016) A review on second and third generation bioethanol production. SAE Technical Paper 2016-36-0515. https://doi.org/10.4271/2016-36-0515
Thompson TM, Young BR, Baroutian S (2019) Advances in the pretreatment of brown macroalgae for biogas production. Fuel Process Technol 195:106151
Tinôco D, Genier HLA, da Silveira WB (2021) Technology valuation of cellulosic ethanol production by Kluyveromyces marxianus CCT 7735 from sweet sorghum bagasse at elevated temperatures. Renew Energy 173:188–196
Yuhendra A, Farghali M, Mohamed IM, Iwasaki M, Tangtaweewipat S, Ihara I, Sakai R, Umetsu K (2021) Potential of biogas production from the anaerobic digestion of Sargassum fulvellum macroalgae: influences of mechanical, chemical, and biological pretreatments. Biochem Eng J 175:108140
Yun EJ, Kim HT, Cho KM, Yu S, Kim S, Choi I-G, Kim KH (2016) Pretreatment and saccharification of red macroalgae to produce fermentable sugars. Bioresour Technol 199:311–318
Acknowledgments
The authors would like to acknowledge funding from the Durban University of Technology (DUT). The financial assistance of the NRF/BRICS STI Grant is hereby acknowledged as well as the consortium partners. We also acknowledge the technical support of the Enzyme Technology Research Group.
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The authors declare no conflict of interest.
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Dlangamandla, N., Permaul, K. (2022). Third-Generation Bioethanol Production Technologies. In: Soccol, C.R., Amarante Guimarães Pereira, G., Dussap, CG., Porto de Souza Vandenberghe, L. (eds) Liquid Biofuels: Bioethanol. Biofuel and Biorefinery Technologies, vol 12. Springer, Cham. https://doi.org/10.1007/978-3-031-01241-9_12
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