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Analytical Techniques to Study Carbohydrates

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The Art of Carbohydrate Analysis

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Abstract

The description of a complex carbohydrate structure includes, in the first instance, monosaccharide composition, anomeric configurations, and the type of glycosidic linkages. To obtain these data, several techniques are available. This chapter describes chemical solvolytic cleavage methods to generate monosaccharides and/or oligosaccharide fragments and discusses, in detail, the chromatographic separation methods to isolate them for further study. Several detection techniques are discussed. Electrophoretic separation of carbohydrates is briefly discussed. A typical flow chart for the structural analysis of carbohydrates from biological samples is presented. Furthermore, protocols for fluorescent or chromophore labeling of glycans are provided.

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  1. CarbExplore Research BV, Groningen, The Netherlands

    Gerrit J. Gerwig

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Gerwig, G.J. (2021). Analytical Techniques to Study Carbohydrates. In: The Art of Carbohydrate Analysis. Techniques in Life Science and Biomedicine for the Non-Expert. Springer, Cham. https://doi.org/10.1007/978-3-030-77791-3_5

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