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Building a PGC-LC-MS N-glycan retention library and elution mapping resource

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Abstract

Porous graphitised carbon-liquid chromatography (PGC-LC) has been proven to be a powerful technique for the analysis and characterisation of complex mixtures of isomeric and isobaric glycan structures. Here we evaluate the elution behaviour of N-glycans on PGC-LC and thereby provide the potential of using chromatographic separation properties, together with mass spectrometry (MS) fragmentation, to determine glycan structure assignments more easily. We used previously reported N-glycan structures released from the purified glycoproteins Immunoglobulin G (IgG), Immunoglobulin A (IgA), lactoferrin, α1-acid glycoprotein, Ribonuclease B (RNase B), fetuin and ovalbumin to profile their behaviour on capillary PGC-LC-MS. Over 100 glycan structures were determined by MS/MS, and together with targeted exoglycosidase digestions, created a N-glycan PGC retention library covering a full spectrum of biologically significant N-glycans from pauci mannose to sialylated tetra-antennary classes. The resultant PGC retention library (http://www.glycostore.org/showPgc) incorporates retention times and supporting fragmentation spectra including exoglycosidase digestion products, and provides detailed knowledge on the elution properties of N-glycans by PGC-LC. Consequently, this platform should serve as a valuable resource for facilitating the detailed analysis of the glycosylation of both purified recombinant, and complex mixtures of, glycoproteins using established workflows.

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Acknowledgements

This study was supported by the Macquarie University Research Excellence Scheme postgraduate scholarship, the Northern Translational Cancer Research Unit through a Cancer Institute NSW competitive student grant, and the National eResearch Collaboration Tools and Resources project (NeCTAR RT016). This research project was facilitated by access to the Australian Proteomics Analysis Facility (APAF) established under the Australian Government’s NCRIS program. We would like to acknowledge Dr. Sophie Zhao, Dr. Ian Walsh and Prof. Pauline Rudd (Bioprocessing Technology Institute, Agency for Science, Technology and Research, Singapore) for supporting the development of GlycoStore.

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  1. Department of Chemistry and Biomolecular Sciences, Faculty of Science & Engineering, Macquarie University, North Ryde, Sydney, NSW, 2109, Australia

    Jodie L. Abrahams, Matthew P. Campbell & Nicolle H. Packer

  2. Institute for Glycomics, Griffith University, QLD, Gold Coast, 4222, Australia

    Jodie L. Abrahams, Matthew P. Campbell & Nicolle H. Packer

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  1. Jodie L. Abrahams
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Correspondence to Nicolle H. Packer.

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Abrahams, J.L., Campbell, M.P. & Packer, N.H. Building a PGC-LC-MS N-glycan retention library and elution mapping resource. Glycoconj J 35, 15–29 (2018). https://doi.org/10.1007/s10719-017-9793-4

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