Abstract
Body fluids are rich sources for proteins originating from organs, tissues, and circulation. They are considered to reflect the state of those tissues and organs and to contain a vast pool of candidate biomarkers for a wide range of conditions. Thus, body fluids are regarded as highly attractive specimens for detecting disease disposition, to monitor markers of progression or to assess the efficacy of treatments. They provide several advantages for screening and clinical testing, such as low cost, minimum to low invasiveness and simple sample collection. However, body fluids are immensely complex mixtures, with most of them containing thousands of proteins and peptides. Therefore, sensitive and accurate high-throughput methods are required for deconvolution of body fluid proteome composition and differential protein abundances. Due to rapid developments in the field of mass spectrometry (MS), MS-based proteomics is deemed to be the most suitable methodology for systematic analysis of body fluid proteomes. In this chapter, we discuss different body fluids and their characteristics, advancements in MS and workflows suitable for body fluid analysis as well as sample preparation and applications in the field of body fluid proteomics and degradomics.
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Acknowledgments
U.a.d.K. acknowledges supported by a Novo Nordisk Foundation Young Investigator Award (NNF16OC0020670) and by an unrestricted research grant from PAUL HARTMANN AG.
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Kalogeropoulos, K., Bundgaard, L., auf dem Keller, U. (2020). Proteomic and Degradomic Analysis of Body Fluids: Applications, Challenges and Considerations. In: Ricard-Blum, S. (eds) Extracellular Matrix Omics. Biology of Extracellular Matrix, vol 7. Springer, Cham. https://doi.org/10.1007/978-3-030-58330-9_8
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