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Capillary Electrophoresis: A New Evolutionary Platform of Plant Secondary Metabolites

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Bioactive Natural Products for Pharmaceutical Applications

Part of the book series: Advanced Structured Materials ((STRUCTMAT,volume 140))

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Abstract

Capillary electrophoresis (c.e) is a modern Spt technique other than any other chromatographic technique for the detection of plant secondary metabolites (Psm). There are two types of c.e such as capillary zone electrophoresis (c.z.e) and micellar electro kinetic (M.e.k.c.c). All kinds of secondary metabolites (Sm) (flavonoids, cardiac glycosides, aglycones, steroids, diterpene, saponin, etc.) are not possible to isolate with the help of HPLC and Gas chromatography. But such type of metabolites may possibly be isolated with the help of c.e smoothly and easily. However, in c.e charged molecules are transferred to the opposite charged molecules in the presence of the electrical field. Very low solvent, low price silica columns, and small amount of samples are needed to run the c.e. There are various characteristics such as voltage (Vtg), temperature, electrolyte concentration, BF pH, micelle concentration, and organic modifiers may influence the Spt of different Sm. In this book chapter we will describe the different parameters of c.e like methodology, detector sample analysis, and combination of other hypnated technique for the detection of metabolites.

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Abbreviations

ANT:

Acetonitrile

Alkd:

Alkaloid

AACT:

Ammonium acetate

BRT:

Borate

BF:

Buffer

BFS:

Buffer solution

C.e:

Capillary electrophoresis

C.w:

Capillary wall

C.z.e:

Capillary zone electrophoresis

C.n:

Coumarin

Esp:

Electrolyte solution parameter

ESOP:

Electro-osmotic pressure

E.c:

Eukaryotic cell

Fvd:

Flavonoid

F.s.c.c:

Fused silica capillary column

I.p:

Instrumental parameters

M.m:

Metabolism

Mtd:

Method

M.e.k.c.c:

Micellar electro kinetic

M.C.L:

Micelle

NI:

Negative ion

Ps:

Plant species

PI:

Positive Ion

P.c:

Prokaryotic cell

Qn:

Quinone

Spt:

Separation

Tps:

Terpenoids

Vtg:

Voltage

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, Guru Ghasidas Viswavidyalaya (A Central University), Bilaspur, Chhattisgarh, 495 009, India

    Dilipkumar Pal & Souvik Mukherjee

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  1. Dilipkumar Pal
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  1. Institute of Pharmaceutical Sciences, Guru Ghasidas Vishwavidyalaya (A Central University), Bilaspur, Chhattisgarh, India

    Dilipkumar Pal

  2. Department of Pharmaceutics, Seemanta Institute of Pharmaceutical Science, Mayurbhanj, Odisha, India

    Amit Kumar Nayak

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Pal, D., Mukherjee, S. (2021). Capillary Electrophoresis: A New Evolutionary Platform of Plant Secondary Metabolites. In: Pal, D., Nayak, A.K. (eds) Bioactive Natural Products for Pharmaceutical Applications. Advanced Structured Materials, vol 140. Springer, Cham. https://doi.org/10.1007/978-3-030-54027-2_8

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