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NMR-Based Metabolomics

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Cancer Metabolomics

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1280))

Abstract

Nuclear magnetic resonance (NMR) spectroscopy is a major analytical method used in the growing field of metabolomics. Although NMR is relatively less sensitive than mass spectrometry, this analytical platform has numerous characteristics including its high reproducibility and quantitative abilities, its nonselective and noninvasive nature, and the ability to identify unknown metabolites in complex mixtures and trace the downstream products of isotope labeled substrates ex vivo, in vivo, or in vitro. Metabolomic analysis of highly complex biological mixtures has benefitted from the advances in both NMR data acquisition and analysis methods. Although metabolomics applications span a wide range of disciplines, a majority has focused on understanding, preventing, diagnosing, and managing human diseases. This chapter describes NMR-based methods relevant to the rapidly expanding metabolomics field.

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Acknowledgments

We gratefully acknowledge the financial support from NIH grants P30CA015704 and RO1GM131491.

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Authors and Affiliations

  1. Northwest Metabolomics Research Center, Anesthesiology and Pain Medicine, University of Washington, Seattle, WA, USA

    G. A. Nagana Gowda & Daniel Raftery

  2. Department of Chemistry, University of Washington, Seattle, WA, USA

    Daniel Raftery

  3. Fred Hutchinson Cancer Research Center, Seattle, WA, USA

    Daniel Raftery

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  1. G. A. Nagana Gowda
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Correspondence to G. A. Nagana Gowda .

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  1. University of California, Los Angeles, Los Angeles, CA, USA

    Shen Hu

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Nagana Gowda, G.A., Raftery, D. (2021). NMR-Based Metabolomics. In: Hu, S. (eds) Cancer Metabolomics. Advances in Experimental Medicine and Biology, vol 1280. Springer, Cham. https://doi.org/10.1007/978-3-030-51652-9_2

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