Abstract
Increasing the sensitivity and throughput of NMR-based metabolomics is critical for the continued growth of this field. In this paper the application of micro-coil NMR probe technology was evaluated for this purpose. The most commonly used biofluids in metabolomics are urine and serum. In this study we examine different sample limited conditions and compare the detection sensitivity of the micro-coil with a standard 5 mm NMR probe. Sample concentration is evaluated as a means to leverage the greatly improved mass sensitivity of the micro-coil probes. With very small sample volumes, the sensitivity of the micro-coil probe does indeed provide a significant advantage over the standard probe. Concentrating the samples does improve the signal detection, but the benefits do not follow the expected linear increase and are both matrix and metabolite specific. Absolute quantitation will be affected by concentration, but an analysis of relative concentrations is still possible. The choice of the micro-coil probe over a standard tube based probe will depend upon a number of factors including number of samples and initial volume but this study demonstrates the feasibility of high-throughput metabolomics with the micro-probe platform.
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Acknowledgments
This research was supported in part by a grant from the National Institute of Environmental Health Sciences (P30ES010126). The authors would like to thank Dr. Dean Olson for valuable discussions on the calculations of mass and concentration sensitivity.
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Grimes, J.H., O’Connell, T.M. The application of micro-coil NMR probe technology to metabolomics of urine and serum. J Biomol NMR 49, 297–305 (2011). https://doi.org/10.1007/s10858-011-9488-2
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DOI: https://doi.org/10.1007/s10858-011-9488-2