Abstract
Cell or nucleus quantification has recently achieved state-of-the-art performance by using convolutional neural networks (CNNs). In general, training CNNs requires a large amount of annotated microscopy image data, which is prohibitively expensive or even impossible to obtain in some applications. Additionally, when applying a deep supervised model to new datasets, it is common to annotate individual cells in those target datasets for model re-training or fine-tuning, leading to low-throughput image analysis. In this paper, we propose a novel adversarial domain adaptation method for cell/nucleus quantification across multimodality microscopy image data. Specifically, we learn a fully convolutional network detector with task-specific cycle-consistent adversarial learning, which conducts pixel-level adaptation between source and target domains and then completes a cell/nucleus detection task. Next, we generate pseudo-labels on target training data using the detector trained with adapted source images and further fine-tune the detector towards the target domain to boost the performance. We evaluate the proposed method on multiple cross-modality microscopy image datasets and obtain a significant improvement in cell/nucleus detection compared to the reference baselines and a recent state-of-the-art deep domain adaptation approach. In addition, our method is very competitive with the fully supervised models trained with all real target training labels.
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Acknowledgement
This research was supported by the National Cancer Institute of the National Institutes of Health under Award Number R21CA237493.
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Xing, F., Bennett, T., Ghosh, D. (2019). Adversarial Domain Adaptation and Pseudo-Labeling for Cross-Modality Microscopy Image Quantification. In: Shen, D., et al. Medical Image Computing and Computer Assisted Intervention – MICCAI 2019. MICCAI 2019. Lecture Notes in Computer Science(), vol 11764. Springer, Cham. https://doi.org/10.1007/978-3-030-32239-7_82
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