Four-vessel occlusion model in rat induces transient forebrain ischemia that resembles cardiac arrest in clinical situations. In this model, two vertebral arteries are coagulated and two common carotid arteries are revers-ibly occluded. This model produces reliable outcome with selective, delayed cell death. For example, CA1 pyramidal neurons in the hippocampus die 2–3 days after ischemia, whereas CA3 neurons survive the same insult. The complications of this model include mortality and seizure. The methods of quantitative analysis of ischemic outcome are introduced. The advantages and limitations of this model are discussed.
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References
Pulsinelli WA, Brierley JB. A new model of bilateral hemispheric ischemia in the unanesthetized rat. Stroke 1979;10(3):267–72
Schmidt-Kastner R, Paschen W, Ophoff BG, Hossmann KA. A modified four-vessel occlusion model for inducing incomplete forebrain ischemia in rats. Stroke 1989;20(7):938–46
Xu ZC, Pulsinelli WA. Responses of CA1 pyramidal neurons in rat hippocampus to transient forebrain ischemia: an in vivo intracellular recording study. Neurosci Lett 1994;171(1–2):187–91
Yamaguchi M, Calvert JW, Kusaka G, Zhang JH. One-stage anterior approach for four-vessel occlusion in rat. Stroke 2005;36(10):2212–4
Ginsberg MD, Busto R. Rodent models of cerebral ischemia. Stroke 1989;20(12): 1627–42
Xu ZC. Neurophysiological changes of spiny neurons in rat neostriatum after transient forebrain ischemia: an in vivo intracellular recording and staining study. Neuroscience 1995;67(4):823–36
Pulsinelli WA, Brierley JB, Plum F. Temporal profile of neuronal damage in a model of transient forebrain ischemia. Ann Neurol 1982;11(5):491–8
Xu ZC, Gao TM, Ren Y. Neurophysiological changes associated with selective neu-ronal damage in hippocampus following transient forebrain ischemia. Biol Signals Recept 1999;8(4–5):294–308
Pulsinelli WA, Jacewicz M. Animal models of brain ischemia. In: Barnett HJM, Stein BM, Mohr JP, Yatsu FM, eds. Stroke: Pathophysiology, Diagnosis, and Management. New York: Churchill Livingstone; 1992:49–64
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Deng, P., Xu, Z.C. (2009). Four-Vessel Occlusion Model in Rats. In: Chen, J., Xu, Z.C., Xu, XM., Zhang, J.H. (eds) Animal Models of Acute Neurological Injuries. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-185-1_6
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DOI: https://doi.org/10.1007/978-1-60327-185-1_6
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