Summary
In the present study we investigated the influence of intron I of the rat renin gene on the transcriptional activity of its promoter in cell culture. The presence of intron I abolished the transcription of reporter genes (luciferase and lacZ) in the non-renin-expressing human embryonic kidney cell line 293, while it did not significantly affect the activity of the rat renin promoter in rat sceletal myoblast line L8 expressing renin. We conclude from these results that intron I of the rat renin gene contains a tissue-specific silencer element probably also responsible for the transcriptional repression of the endogeneous renin gene in 293 cells.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bader M, Lee MA, Zhao Y, Böhm M, Bachmann J, Sander M, Djavidani B, Bachmann S, Zimmermann F, Wilbertz J, Zeh K, Wagner J, Peters J, Ganten D. Renin gene expression and hypertension in transgenic animais. In: Raizada MK, Phillips MI, Sumners C, eds. Cellular and Molecular Biology of the Renin-Angiotensin System. Boca Raton, CRC Press, 1993: 60–93).
Bader M, Paul M, Fernandez-Alfonso M, Kaling M, Ganten D. Molecular biology and biochemistry of the renin-angiotensin system. In: Swales JD, ed. Textbook of Hypertension. Blackwell, Oxford, 1994: 214–232).
Borensztein P, Germain S, Fuchs S, Philippe J, Corvol P, Pinet F. Cis-regulatory elements and trans-acting factors directing basal and cAMP-stimulated human renin gene expression in chorionic cells. Circ Res 1994; 74: 764–773.
Burt DW, Mullins LJ, George H, Smith G, Brooks J, Pioli D, Brammar WJ. The nucleotide sequence of a mouse renin-encoding gene, Ren-1d, and its upstream region. Gene 1989a; 84: 91–104.
Burt DW, Nakamura N, Kelley P, Dzau VJ. Identification of negative and positive regulatory elements in the human renin gene. J Biol Chem 1989b; 264: 7357–7362.
De Wet JR, Wood KV, Helinski DR, DeLuca M. Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli. Proc Natl Acad Sci USA 1985; 82: 7870–7873.
Dene H, Wang SM, Rapp JP. Restriction fragment length polymorphisms for the renin gene in Dahl rats. J Hypertens 1989; 7: 121–126.
Fukamizu A, Nishi K, Nishimatsu S, Miyazaki H, Hirose S, Murakami K. Human renin of renin-secreting tumor. Gene 1986; 49: 139–145.
Fukamizu A, Nishi K, Cho T, Saitoh M, Nakayama K, Ohkubo H, Nakanishi S, Murakami K. Structure of the rat renin gene. J Mol Biol 1988; 201: 443–450.
Gorman CM, Merlino GT, Willingham MC, Pastan I, Howard BH. The rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection. Proc Natl Acad Sci USA 1982; 79: 6777–6781.
Horton W, Miyashita T, Kohno K, Hassell JR, Yamada Y. Identification of a phenotype-specific enhancer in the first intron of the rat collagen II gene. Proc Natl Acad Sci USA 1987; 84: 8864–8868.
Klemm S, Ballantine D, Tunny T, Stowasser M, Gordon R. Different frequencies of renin gene RFLP in patients with angiotensin-responsive and angiotensin-unresponsive aldosterone-producing adenomas (APA). J Hypertension 1994;12(Suppl.3):S189(Abstract).
Nakamura N, Burt DW, Paul M, Dzau VJ. Negative control elements and cAMP responsive sequences in the tissue-specific expression of mouse renin genes. Proc Natl Acad Sci USA 1989; 86: 56–59.
Rossi P, DeCrombrugghe B. Identification of a cell-specific transcriptional enhancer in the first intron of the mouse a2 (type I) collagen gene. Proc Natl Acad Sci USA 1987; 84: 5590–5594.
Sigmund CD, Jones CA, Fabian JR, Mullins JJ, Gross KW. Tissue and cell specific expression of a renin promoter-reporter gene construct in transgenic mice. Biochem Biophys Res Commun 1990; 170: 344–350.
Sola C, Tronik D, Dreyfus M, Babinet C, Rougeon F. Renin-promoter SV40 large T-antigen transgenes induce tumors irrespective of normal cellular expression of renin genes. Oncogene Res 1989; 5: 149–153.
Sun J, Oddoux C, Lazarus A, Gilbert MT, Catanzaro DF. Promoter activity of human renin 5′-flanking DNA sequences is activated by the pituitary-specific transcription factor Pit-1. J Biol Chem 1993; 268: 1505–1508.
Tamura K, Tanimoto K, Murakami K, Fukamizu A. A combination of upstream and proximal elements is required for efficient expression of the mouse renin promoter in cultured cells. Nucleic Acids Res. 1993; 20: 3617–3623.
Tronik D, Dreyfus M, Babinet C, Rougeon F. Regulated expression of the Ren-2 gene in transgenic mice derived from parental strains carrying only the Ren-1 gene. EMBO J 1987; 6: 983–987.
Wang J, Chen S. Identification of a promotor and a silencer at the 3′-end of the first intron of the human aromatase gene. Mol Endocrinol 1992; 6: 1479–1488.
Wang SM, Rapp JP. Structural differences in the renin gene of Dahl salt-sensitive and salt-resistant rats. Mol Endocrinol 1989; 3: 288–294.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Springer Science+Business Media New York
About this chapter
Cite this chapter
Voigtländer, T., Ripperger, A., Ganten, D., Bader, M. (1995). Transcriptional Silencer in Intron I of the Rat Renin Gene. In: Mukhopadhyay, A.K., Raizada, M.K. (eds) Tissue Renin-Angiotensin Systems. Advances in Experimental Medicine and Biology, vol 377. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0952-7_18
Download citation
DOI: https://doi.org/10.1007/978-1-4899-0952-7_18
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4899-0954-1
Online ISBN: 978-1-4899-0952-7
eBook Packages: Springer Book Archive