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Molecular Epidemiologic Analysis of Antibiotic Resistant Microorganisms

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Antimicrobial Resistance

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 390))

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Abstract

Antibiotic resistance presents a specific, readily available marker to use in the epidemiologic analysis of microorganisms. Historically, most epidemics like bubonic plague, cholera, influenza, and meningococcal infection involved microorganisms not subjected to the selective pressures of therapeutic antimicrobials. For such outbreaks, there were no antimicrobial resistance markers typifying the outbreak strains. Modern antimicrobial chemotherapy has brought with it selective pressures that favor emergence of resistant clones, whose phenotypic traits thereby provide the epidemiologist and clinical microbiologist with distinctive phenotypic and genotypic traits in these microorganisms.

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References

  1. F.R.J. Schmidt, E.J Nucken, and R.B. Henschke. Nucleotide sequence analysis of 2’ aminoglycoside nucelotidyl-transferase ANT(2“) from Tn4000; its evolving relationship with AAD(3”) and impact on evolution. Molec Microbiol 1988; 2: 709–717.

    Article  CAS  Google Scholar 

  2. J.N. Maslow, M.E. Mulligan, and R.D. Arbeit. Molecular epidemiology: application of contemporary techniques to the typing of microorganisms. Clin Infect Dis 1993; 17: 153–164.

    Article  PubMed  CAS  Google Scholar 

  3. J.F. John and J.A. Twitty. Plasmid as epidemiologic markers in nosocomial gram-negative bacilli: expereince at a university hospital and review of the literature. Rev Infect Dis 1986; 8: 693–704.

    Article  PubMed  CAS  Google Scholar 

  4. R.D. Arbeit. Molecular methods for the clinical microbiology laboratory. In: Hallows A, Hausler WJ, Kerrmann KLI H.D., Shadom HJ, ed. Manual of Clin Microbiol. 1994: vol 6th edition).

    Google Scholar 

  5. B. Swaminathan and G.M. Matar. In: Persing DH, ed. Diagnostic Molecular Microbiology. Principles and Applications. American Society for Microbiology, 1993:

    Google Scholar 

  6. E. Bingen. Applications of molecular methods to epidemiologic investigations of nosocomial infections in a pediatric hospital. Infect Control Hosp Epidemiol 1994; 15: 488–493.

    Article  PubMed  CAS  Google Scholar 

  7. J.M. Musser, D.A. Bemis, H. Ishikawa, and R.K. Selander. Clonal diversity and host distribution in Bordetella bronchiseptica. J Bacteriol 1987; 169 (6): 2793–2803.

    PubMed  CAS  Google Scholar 

  8. S. Falkow. Infectious Multiple Drug Resistance. In: Ltd P, ed. London: 1975

    Google Scholar 

  9. C.I. Kado and S.T. Liu. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol 1981; 1: 1365–1373.

    Google Scholar 

  10. L.P. Elwell, J. deGraaff, D. Seibert, and S. Falkow. Plasmid-linked ampicillin resistance in Hemophilus influenzae type G. Infect Immun 1975; 12: 404–410.

    PubMed  CAS  Google Scholar 

  11. M. Singh and J.F. John. Isomeric DNA ladder formation of a plasmid encoding tobramycin resistance from Escherichia coli. J Antimicrob Chemother 1991; 28: 627–637.

    Article  PubMed  CAS  Google Scholar 

  12. D. Sirot, C. Chanal, R. Labia, M. Meyran, J. Sirot, and R. Cluzel. Comparative study of five plasmid-mediated ceftazidimases isolated in Kelbsiells pneumoniae. J Antimicrob Chemother 1989; 24: 509–521.

    Article  PubMed  CAS  Google Scholar 

  13. C.E. Rubens, W.E. Farrar, Z.A. McGee, and W. Schaffner. Evolution of a plasmid mediating resistance to multiple antimicrobial agents during a prolonged epidemic of nosocomial infections. J Infect Dis 1981; 143: 170–181.

    Article  PubMed  CAS  Google Scholar 

  14. T.F. O’Brien, J.D. Hopkins, E.S. Gilleece, et al. Molecular epidemiology of antibiotic resistance in salmonella from animals and human beings in the United States. New Engl J Med 1982; 307: 1–6.

    Article  PubMed  Google Scholar 

  15. S.D. Holmberg, I.K. Wachsmuth, F.W. Hickman-Brenner, P.A. Blake and M.L. Cohen. Comparison of plasmid profile analysis, phage typing, and antimicrobial susceptibility tsting in characterizing Salmonella typhimurium isolates from outbreaks. J Clin Microbiol 1981; 19: 100–104.

    Google Scholar 

  16. D.R. Schaberg, C.E. Tompkins LSR, and S. Flakow. R plasmids and nosocomial infection. In: Rolyce CSaKR, ed. Plasmids and Transposons Environmental Effects and Manitenance Mechanisms. New York: Academic Press, 1980: 43–55.

    Google Scholar 

  17. J.F. John, R.J. Sharbaugh, and E.R. Bannister. Enterobacter cloacae: bacteremia, epidemiology, and antibiotic resistance. Rev Infect Dis 1982; 413–28.

    Google Scholar 

  18. C.R. Clabots, L.R. Peterson, and D.N. Gerding. Characterization of a nosocomial Clostridium docile outbreak by using plasmid profile typing and clindamycin susceptibility testing. J Infect Dis 1988; 158: 731–736.

    Article  PubMed  CAS  Google Scholar 

  19. Bialkowska, H. Hobruzanska, P. Jaskot, and O. Hammerberg. Evaluation of restriction endonuclear fingerprinting of chromosomal DNA and plasmid profile analysis for characterization of multi-resistant coagulase-negative staphylococci in bacteremic neonates. J Clin Microbiol 1990; 28: 269–275.

    Google Scholar 

  20. R.J. Owen. Chromosomal DNA fingerprinting-a new method of species and strain identification applicable to microbial pathogens. J Med Microbiol 1989; 30: 89–99.

    Article  PubMed  CAS  Google Scholar 

  21. E. Bingen, E. Denamur, N. Lambert-Zechovsky, N. Brahimi, M. El Lakany, and J. Elion. Rapid genotyping shows the absence of cross-contamination in Enterobacter cloacae nosocomial infections. J Hosp Infect 1992; 21: 95–101.

    Article  PubMed  CAS  Google Scholar 

  22. T.L. Stull, J.J. LiPuma, and T.D. Edling. A broad-spectrum probe for molecular epidemiology of bactreria: ribosomal RNA. J Infect Dis 1988; 157: 280–286.

    Article  PubMed  CAS  Google Scholar 

  23. E.H. Bingen, E. Denamur, and J. Elion. Use of ribotyping in epidemiological surveillance of nosocomial outbreaks. Clin Microbiol Rev 1994; 7: 311–327.

    PubMed  CAS  Google Scholar 

  24. I. Otal, C. Martin, V. Vincent-Levy-Frebault, D. Thierry, and B. Gicquel. Restriction fragment length polymorphism analysis using IS6110 as an epidemiological marker in tuberculosis. J Clin Microb 1991; 29: 1252–1254.

    CAS  Google Scholar 

  25. V.G. Coronado, C.N. Beck-Sauge, M.D. Hutton, et al. Transmission of multidrug-resistant Mycobacterium tuberculosis among persons with human immunodeficiency virus infection in an urban hospital: epidemiologic and restricition fragment lenth polymorphism analysis. J Infect Dis 1993; 168: 1052–1055.

    Article  PubMed  CAS  Google Scholar 

  26. A. Genewein, A. Telenti, C. Bernasconi, et al. Molecular approach to identifying route of transmission of tuberculosis in the community. Lancet 1993; 342: 841–844.

    Article  PubMed  CAS  Google Scholar 

  27. M.P. Bratoeva, J.F. John, and N.L. Barg. Molecular epidemiology of Trimethoprim-resistant Shigella boydii serotype 2 strains from Bulgaria. J Clin Microbiol 1992;:1428–1431.

    Google Scholar 

  28. J.R. Lupski and G.M. Weinstock. Short, interspersed repetitive DNA sequences in prokaryotic genomes. J Bacteriol 1992; 174: 4525–4529.

    PubMed  CAS  Google Scholar 

  29. A.C. Fluit, A.T.A. Boa, and J. Verhoef. A probe for the detection of methicillin-resistant Staphylococcus aureus. Eur J Clin Microbiol Infect Dis 1990; 9: 605–608.

    Article  PubMed  CAS  Google Scholar 

  30. F.C. Tenover, K. Arbeit, and G. Archer. Comparison of traditional and molecular methods of typing isolates Staphylococcus aureus. J Clin Microbiol 1994; 32: 407–415.

    PubMed  CAS  Google Scholar 

  31. M.J. Storrs, P. Courvalin, and T.J. Foster. Genetic analysis of gentamicin resistance in methicillin-and gentamicin-resistant strains of Staphylococcus aureus isolated in Dublin hospitals. Antimicrob Agents Chemother 1988; 32: 1174–1181.

    Article  PubMed  CAS  Google Scholar 

  32. J.J. Weems, J.H. Lowrance, L.M. Baddour, and W.A. Simpson. Molecular epidemiology of nosocomial, multiply aminoglycoside resistant Enterococcus faecalis. J Antimicrob Chemother 1989; 24: 121–130.

    Article  PubMed  CAS  Google Scholar 

  33. A. Pilantanapak, A. Bhumiratana, P. Jayanetra, and W. Panbangred. Biotinylated probes for epidemiological studies of drug resistance in Salmonella krefeld. J Antimicrob Chemother 1990; 25: 593–603.

    Article  PubMed  CAS  Google Scholar 

  34. J.E. Degener, M.E.O.C. Heck, W.J. VanLeewwen, et ai. Nosocomial infection by Staphylococcus helolyticus and typing methods for epidemiological study. J Clin Microbiol 1994; 32: 2260–2265.

    PubMed  CAS  Google Scholar 

  35. C. Mabilat and P. Courvalin. Development of “oligotyping” for characterization and molecular epidemiology of TEM beta-lactamases in members of the family Enterobacteriaceae. Antimicrob Agents Chemother 1990; 34: 2210–2216.

    Article  PubMed  CAS  Google Scholar 

  36. M. Boissinot, A. Huot, J. Mercier, and R.C. Levesque. Development of gene probes and evolutionary relationships of the PSE-4 bla gene to plasmid-mediated B-lactamases of gram-negative bacteria. Mol Cell Probes 1989; 3: 179–188.

    Article  PubMed  CAS  Google Scholar 

  37. J. Levy, G. Verhaegen, P. De Mol, M. Couturier, D. Dekegel, and J.P. Butzler. Molecular characterization of resistance plasmids in epidemiologically unrelated strains of multiresistant Haemophilus influenzae. J Infect Dis 1993; 168: 177–187.

    Article  CAS  Google Scholar 

  38. P. Richard, R. LeFloch, C. Chamoux, M. Pannier, E. Espaze, and H. Richet. Pseudomonas aeruginosa outbreak in a burn unit: role of antimicrobials in the emergence of multiply resistant strains. J Infect Dis 1994; 170: 377–383.

    Article  PubMed  CAS  Google Scholar 

  39. M.C. Roberts, L.V. McFarland, P. Mullany, and M.E. Mulligan. Characterization of the genetic basis of antibiotic resistance in Clostridium domicile. J Antimicrob Chemother 1994; 33: 419–429.

    Article  PubMed  CAS  Google Scholar 

  40. C.M. Kell, J.Z. Jordens, M. Daniels, et al. Molecular epidemiology of penicillin-resistant pneumococci isolated in Nairobi, Kenya. Infect Immun 1993; 616 (10): 4382–4391.

    Google Scholar 

  41. P. Bernard-Matsiota, D. Thierry, J.L. Guesdon, and C. Nauciel. Molecular epidemiology of Legionalla penumophila serogroup 1 by ribotyping with a non-radioactive probe and PCR fingerprinting. FEMS 1994; 9: 23–28.

    Google Scholar 

  42. J. Welsh and M. McClelland. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 1990;18(7213–7218).

    Google Scholar 

  43. F.C. Fang, M. McClelland, D.G. Guiney, et al. Value of molecular epidemiologic analysis in a nosocomial methicillin-resistant Staphylococcus aureus outbreak. JAMA 1993; 270 (11): 1323–1328.

    Article  PubMed  CAS  Google Scholar 

  44. M.J. Struelens, E. Carlier, N. Maes, E. Serruys, W.G.V. Quint, and A. van Belkum. Nosocomial colonization and infection with multiresistant Acinetobacter baumannii: outbreak delineation using DNA macrorestriction analysis and PCR-fingerprinting. J Hosp Infect 1993; 25: 15–32.

    Article  PubMed  CAS  Google Scholar 

  45. R.V. Goering. Molecular epidemiology of nosocomial infection: analysis of chromosomal restriction fragment patterns by pulsed-field gel electrophoresis. Infect Conrol Hosp epidemiol 1993; 14: 595–600.

    Article  CAS  Google Scholar 

  46. R.D. Arbeit. Laboratory procedures for the epidemiologic analysis of microorganisms. In: Murray PR et al, ed. Manual of Clinical Microbiology. 6 ed. Washington, D.C.: American Society for Microbiology, 1994:

    Google Scholar 

  47. L. Soldata and J.C. Piffaretti. Molecular typing of Shigella strains using pulsed field gel electrophoresis and genome hybridization with insertion sequences. Res Microbiol 1991; 142: 489–498.

    Article  Google Scholar 

  48. H.S. Sader, A.C. Pignatari, R. Frei, R.J. Hollis, and R.N. Jones. Pulsed-field gel electrophoresis of restriction-digested genomic DNA and antimicrobial susceptibility of Xanthomonas maltophilia strains from Brazil, Switzerland and the USA. J Antimicrob Chemother 1994; 33: 615–618.

    Article  PubMed  CAS  Google Scholar 

  49. E.S. Go, C. Urgan, J.Burns, B. Breiswirth, W. Wisner, N. Mariano, M. Mosinka-Snipas, and J.J. Rahal. Clinical and molecular epidemiology of acinetobacter infections sensitive only to polymyxin B and sulbactam. Lancet 1994; 344: 1329–32.

    CAS  Google Scholar 

  50. A.C. Reboli, E.D. Houston, J.S. Moteforte, C.A. Wood, and R.J. Hammill. Discrimination of epidemic and sporadic isolates of Acinetobacter baumannii by repetitive element PCR-mediated DNA fingerprinting. J Clin Microbiol 1994; 32: 2635–40.

    PubMed  CAS  Google Scholar 

  51. G. Prevost, B. Pottecher, M. Dahlet, M. Bientz, J.M. Manta, and Y. Piemont. Pulsed field gel electrophoresis as a new epidemiological tool for monitoring methicillin-resistant Staphylococcus aureus in an intensive care unit. J Hosp Infect 1991; 17: 255–269.

    Article  PubMed  CAS  Google Scholar 

  52. L.A. Herwaldt and L.D.P. Boyken. M.A. Biotyping of coagulase-negative staphylococci: 108 isolates from nosocomial bloodstream infections. Diagn Microbiol Infect Dis 1990; 13: 461–466.

    Article  PubMed  CAS  Google Scholar 

  53. J.F. John, T.J. Grieshop, L.M. Atkins, and C.G. Platt. Widespread colonization of personnel at a Veterans Affairs Medical Center by methicillin-resistant, coagulase-negative staphylococcus. Clin Infect Dis 1993; 17: 380–388.

    Article  PubMed  Google Scholar 

  54. W.E. Keene, McAnulty JM, Koesly FC, et al. A swimming-associated outbreak of hemorrhagic colitis caused by Escherichia coli 0157;H7 and Shigella sonnet. N Engl J Med 1994; 331 (9): 579–584.

    Article  PubMed  CAS  Google Scholar 

  55. C.H. Patrick, J.F. John, A.H. Levkoff, and L.M. Atkins. Relatedness of strains of methicillin-resistant coagulase-negative Staphylococcus colonizing hospital personnel and producing bacteremias in a neonatal intensive care unit. Pediatr Infect Dis J 1992; 11: 935–940.

    Article  PubMed  CAS  Google Scholar 

  56. B.M. Willey, A.J. McGeer, M.A. Ostrowski, B.N. Kresiwirth, and D.E. Low. The use of molecular typing techniques in the epidmiologic investigation of resistant enterococci. Infect Control Hosp Epidemiol 1994; 15: 548–556.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Molecular Genetics and Microbiology, Robert Wood Johnson Medical School, New Brunswick, NJ, USA

    Joseph F. John Jr. M.D. (Professor of Medicine)

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  1. Joseph F. John Jr. M.D.
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Editors and Affiliations

  1. Thomas Jefferson University Hospital, Philadelphia, Pennsylvania, USA

    Donald L. Jungkind

  2. St. Christopher’s Hospital for Children, Philadelphia, Pennsylvania, USA

    Joel E. Mortensen

  3. The Graduate Hospital, Philadelphia, Pennsylvania, USA

    Henry S. Fraimow

  4. Merck Research Laboratories, West Point, Pennsylvania, USA

    Gary B. Calandra

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© 1995 Springer Science+Business Media New York

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John, J.F. (1995). Molecular Epidemiologic Analysis of Antibiotic Resistant Microorganisms. In: Jungkind, D.L., Mortensen, J.E., Fraimow, H.S., Calandra, G.B. (eds) Antimicrobial Resistance. Advances in Experimental Medicine and Biology, vol 390. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9203-4_13

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  • DOI: https://doi.org/10.1007/978-1-4757-9203-4_13

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  • Online ISBN: 978-1-4757-9203-4

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