Skip to main content
Log in

A probe for the detection of methicillin-resistantStaphylococcus aureus

  • Notes
  • Published:
European Journal of Clinical Microbiology and Infectious Diseases Aims and scope Submit manuscript

Abstract

An approximately 300 base pair DNA fragment for use as a probe was isolated from methicillin-resistantStaphylococcus aureus DNA partially digested withSau3AI. This probe hybridized with 25 methicillin-resistant clinical isolates ofStaphylococcus aureus belonging to 18 different phage types, but not with 41 clinical isolates susceptible to methicillin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Locksley RM, Cohen ML, Quinn TC, Tompkins LS, Coyle MB, Kirihara JM, Counts GW: Multiply antibiotic-resistantStaphylococcus aureus: introduction, transmission and evolution of nosocomial infection. Annals of Internal Medicine 1982, 97: 317–324.

    PubMed  Google Scholar 

  2. Bradley JM, Noone P, Townsend DE, Grubb WB: Methicillin-resistantStaphylococcus aureus in a London hospital. Lancet 1985, i: 1493–1495.

    Article  Google Scholar 

  3. Sjöström J-E, Löfdahl S, Philipson L: Transformation reveals a chromosomal locus of gene(s) for methicillin resistance inStaphylococcus aureus. Journal of Bacteriology 1975, 123: 905–915.

    PubMed  Google Scholar 

  4. Berger-Bächi B, Kohler ML: A novel site on the chromosome ofStaphylococcus aureus influencing the level of methicillin resistance: genetic mapping. FEMS Microbiology Letters 1983, 20: 305–309.

    Article  Google Scholar 

  5. Beck WD, Berger-Bächi B, Kayser FH: Additional DNA in methicillin-resistantStaphylococcus aureus and molecular cloning ofmec-specific DNA. Journal of Bacteriology 1986, 165: 373–378.

    PubMed  Google Scholar 

  6. Matthews PR, Reed KC, Stewart PR: The cloning of chromosomal DNA associated with methicillin and other resistances inStaphylococcus aureus. Journal of General Microbiology 1987, 133: 1919–1929.

    PubMed  Google Scholar 

  7. Hackbarth CJ, Chambers HF: Methicillin-resistant staphylococci: genetics and mechanisms of resistance. Antimicrobial Agents and Chemotherapy 1989, 33: 991–994.

    PubMed  Google Scholar 

  8. Report Subcommittee Staphylococcal Phage Typing. National Journal of Systemic Bacteriology 1975, 25: 241–242.

    Google Scholar 

  9. Van Leeuwen WJ, Rost JA: An additional set of phages for the typing ofStaphylococcus aureus strains of human origin and non-typable with the international basic set of phages. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene 1976, Supplement 5: 1013–1019.

    Google Scholar 

  10. Lindberg M, Sjöström J-E, Johansson T: Transformation of chromosomal and plasmid characters inStaphylococcus aureus. Journal of Bacteriology 1972, 109: 844–847.

    PubMed  Google Scholar 

  11. Maniatis T, Fritsch EF, Sambrook J: Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1982.

    Google Scholar 

  12. Welcher AA, Torres AR, Ward DC: Selective enrichment of specific DNA, cDNA, and mRNA sequences using biotinylated probes, avidin and copper-chelate agarose. Nucleic Acids Research 1986, 14: 10027–10036.

    PubMed  Google Scholar 

  13. Fluit AC, Baas PD, Van Boom JH, Veeneman GH, Jansz HS: Gene A protein cleavage of recombinant plasmids containing the ØX174 replication origin. Nucleic Acids Research 1984, 12: 6443–6454.

    PubMed  Google Scholar 

  14. Matsuhashi M, Song MD, Ishino F, Wachi M, Doi M, Inoue M, Ubukata K, Yamashita N, Konno M: Molecular cloning of the gene of a penicillin-binding supposed to cause high resistance to β-lactam anti-biotics inStaphylococcus aureus. Journal of Bacteriology 1986, 167: 975–980.

    PubMed  Google Scholar 

  15. Song MD, Wachi M, Doi M, Ishino F, Matsuhashi M: Evolution of an inducible penicillin-target protein in methicillin-resistantStaphylococcus aureus by gene fusion. FEBS Letters 1987, 221: 167–171.

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fluit, A.C., Box, A.T.A. & Verhoef, J. A probe for the detection of methicillin-resistantStaphylococcus aureus . Eur. J. Clin. Microbiol. Infect. Dis. 9, 605–608 (1990). https://doi.org/10.1007/BF01967216

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01967216

Keywords

Navigation