Abstract
Photodynamic therapy (PDT) is a treatment for various malignant and benign lesions using light-activated photosensitising drugs in the presence of molecular oxygen. PDT causes tissue damage by a combination of processes involving the production of reactive oxygen species (in particular singlet oxygen), which can directly induce cell killing1, or indirectly via disruption of the tissue microvasculature2. Since the cytotoxic effect relies on the presence of oxygen, monitoring of tissue oxygenation both during and after PDT is important for understanding the basic physiological mechanisms and dosimetry of PDT3,4. Furthermore, it is known that the tumour destruction can be limited by the amount of available oxygen5, 6. During irradiation, changes in tissue oxygenation occur due to PDT-induced vasoconstriction and oxygen consumption in photodynamic reactions. Thereby tissue oxygenation can be reduced to levels insufficient for any further tumour destruction7, 8. In order to prevent a significant reduction in available oxygen levels, online real time monitoring could be useful during treatment.
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References
M. D. Mason, Cellular aspects of photodynamic therapy for cancer. Rev. Contemp. Pharmacother. 10, 25 - 37 (1999).
E. Ben-Hur, A. Orenstein, The endothelium and red blood cells as potential targets in PDT-induced vascular stasis. Int. J. Radial Biol. 60, 293 - 301 (1991).
V. H. Fingar, T. J. Wieman, S. W. Taber, P. Singh, S. J. Kempf, C. G. Pietsch and C. Maldonado, Use of scanning doppler velocimetry to monitor vascular changes during photodynamic therapy. SPIE 3592, 1419 (1999).
Q. Chen, H. Chen and F. W. Hetzel, Tumor oxygenation changes post-photodynamic therapy. Photochemistry and Photobiology, 63, 128 - 131 (1996).
B. W. Henderson, and V.H. Fingar, Oxygen limitation of direct tumor cell kill during photodynamic treatment of a murine tumor model. Photochem. Photobiol. 49, 299 - 304 (1989).
B. W. Henderson, and V. H. Fingar, Relationship of tumor hypoxia and response to Photodynamic treatment in an experimental mouse tumor. Cancer Res. 47, 3110 - 3114 (1987).
T. M. Sitnik, J. A. Hampton, and B. W. Henderson, Reduction of tumour oxygenation during and after photodynamic therapy in vivo: effects of fluence rate. Br. J. Cancer. 77, 1386 - 1394 (1998).
B. W. Henderson, T. M. Busch, L. A. Vaughan, N. P. Frawley, D. Babich, T. A. Sosa, J. D. Zollo, Dee, A. S., Cooper, M. T., Bellnier, D. A., W. R. Greco, and A. R. Oseroff, Photofrin Photodynamic Therapy can significantly deplete or preserve oxygenation in human basal cell carcinomas during treatment, depending on fluence rate. Cancer Res. 60, 525 - 529 (2000).
H. Messman, P. Mlkvy, G. Buonaccorsi, C. L. Davies, A. J. MacRobert and S. G. Bown, Enhancement of photodynamic therapy with 5-aminoaevulinic acid-induced porphyrin photosensitisation in normal rat colon by threshold and light fractionation studies. Br. J. Cancer.72, 589 - 594 (1995).
S.G. Bown, C. J. Tralau, P. D. Coleridge-Smith, D. Akdemir and T. J. Weiman, Photodynamic therapy with porphyrin and phthalocyanine sensitisation: Quantitative studies in normal rat liver. British Journal of Cancer, 54, 43 (1986).
B. W. McIlroy, A. Cumow, G. Buonaccorsi, M. A. Scott. S. G. Bown, and A. J. MacRobert, Spatial measurement of oxygen levels during photodynamic therapy using time-resolved optical spectroscopy. J. Photochem. Photobiol. B. 43, 47 - 55 (1998).
F. Steinberg, H. J. Rohrborn, T. Otto, K. M. Scheufler, and C. Streffer, NIR reflection measurements of hemoglobin and cytochrome aa3 in healthy tissue and tumors. Correlations to oxygen consumption: preclinical and clinical data, in: Oxygen Transport to Tissue XIX, edited by Harrison, and D. T. Delpy, ( Plenum Press, New York, 1997 ), pp. 69 - 76.
F. Steinberg, H. J. Rohrbom, K. M. Scheufler, S. Asgari, H. A. Trost, V. Seifert, D. Stolke and C. Streffer NIR reflection spectroscopy based oxygen measurements and therapy monitoring in brain tissue and intracranial neoplasms. Correlation to MRI and angiography, in: Oxygen Transport to Tissue XIX, edited by, Harrison and D. T. Delpy, ( Plenum Press, New York, 1997 ), pp. 553 - 560.
C. D. Gomersall, P. L. Leung, T. Gin, G. M. Joynt, R. J. Young, W. S. Poon, and T. E. Oh, A comparison of the Hamamatsu NIRO 500 and the INVOS 3100 near-infrared spectrophotometers. Anaesth. Intensive Care. 26, 548 - 557 (1998).
K. H. Frank, M. Kessler, K. Appelbaum, and W. Dummler, The Erlangen micro-lightguide spectrophotometer EMPHO I. Phys. Med. Biol. 34, 1883 - 1900 (1989).
J. Mayhew, D. Johnston, J. Berwick, M. Jones, P. Coffey, and Y. Zheng, Spectroscopic analysis of neural activity in brain: Increased oxygen consumption following activation of barrel cortex. Neuroimage, 13, 540 - 543 (1991).
D. A. Bellnier, W. R. Potter, L. A. Vaughan, T. M. Sitnik, J.C. Parsons, W. R. Greco, J. Whitaker, P. Johnson and B. W. Henderson, The validation of a new vascular damage assay for photodynamic therapy agents. Photochem. Photobiol. 62, 896 - 905 (1995).
Z. Chen, T. E. Milner, X. Wang, S. Srinivas and J. S. Nelson, Optical Doppler Tomography: Imaging in vivo blood flow dynamics following pharmacological intervention and photodynamic therapy. Photochem. Photobiol. 67, 56 - 60 (1998).
V. H. Fingar, T. J. Wieman, S. A. Wiehle, and P. B. Cerrito, The Role of Microvascular Damage in Photodynamic Therapy: The Effect of Treatment on Vessel Constriction, Permeability, and Leukocyte Adhesion. Cancer Research, 52, 4914 - 4921 (1992).
H. Barr, Photodynamic therapy in the normal rat colon with phthalocyanine sensitisation. British Journal of Cancer. 56, 111 - 118 (1987).
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Woodhams, J.H., Kunz, L., Bown, S.G., MacRobert, A.J. (2003). Monitoring the Effect of PDT on in Vivo Oxygen Saturation and Microvascular Circulation. In: Thorniley, M., Harrison, D.K., James, P.E. (eds) Oxygen Transport to Tissue XXV. Advances in Experimental Medicine and Biology, vol 540. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-6125-2_33
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DOI: https://doi.org/10.1007/978-1-4757-6125-2_33
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