31Phosphorus NMR Studies of Mercuric Chloride Nephrotoxicity in the in Vitro Perfused Rat Kidney
31P Nuclear Magnetic Resonance (NMR) studies of the effect of hypoxia on ATP levels in the isolated perfused rat kidney have shown that a step-wise decrease in oxygen delivery is associated with a step-wise decrease in ATP to a new plateau level (1). The decrement in ATP was closely correlated with the volume extent of cellular necrosis determined from morphometric analysis of the same kidneys at the end of the perfusion period.
KeywordsMercuric Chloride Methylene Diphosphonate Fractional Sodium Excretion Hyperoncotic Albumin Krebs Bicarbonate Buffer
Unable to display preview. Download preview PDF.
- 1.Z.H. Endre, J.G.G. Ledingham, G.K. Radda, P.J. Ratcliffe and J.D. Tange, J. Phvsiol., 384:41 (1987)Google Scholar
- 3.P.J. Ratcliffe, Z.H. Endre, L.G. Nicholls, J.D. Tange and J.G.G. Ledingham, The isolated perfused rat kidney: filtering and non-filtering models in the assessment of altered renal vascular resistance in nephrotoxicity, (this Symposium).Google Scholar
- 5.H.J. Schureck and W. Kriz, Lab. Invest., 53:145 (1985)Google Scholar
- 6.P.J. Ratcliffe, Z.H. Endre, S.J. Scheinmann, J.D. Tange, J.G.G. Ledingham and G.K. Radda, Phosphorus nuclear magnetic resonance study of steady state ATP levels during graded hypoxia in the isolated perfused rat kidney, submitted for publicationGoogle Scholar
- 7.Z. H. Endre, L.G. Nicholls, P.J. Ratcliffe and J.G.G. Ledigham, Prevention and reversal of mercuric choloride-induced increases in renal vascular resistance (RVR) by captoprl,(this Sysmposium)Google Scholar