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Structural and Functional Properties of Consecutive Enzymes in the Glycolytic Pathway

  • Herman C. Watson
Part of the Progress in Mathematics book series (NSSA)

Abstract

In this article I will outline the basic mechanistic features of triosephosphate dehydrogenase (GPD), phosphoglycerate kinase (PGK) and phosphoglycerate mutase (PGM); enzymes whose structures have been studied by me and by my coworkers over many years. Emphasis will be placed on the properties of these enzymes which appear to be functionally important as seen from the static — snap shot — type pictures observed using the analysis of X-ray diffraction data. The information provided will show that great strides have been made towards our understanding of the enzymic processes involved but that much remains to be done to confirm the proposed reaction mechanisms.

Keywords

Phosphoryl Group Phosphoglycerate Mutase Propose Reaction Mechanism Phosphoryl Transfer Covalent Intermediate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Banks, R.D., Blake, C.C.F., Evans, P.R., Haser, R., Rice, D.W., Hardy, G.W., Merrett, M. & Phillips, A.W. (1979) Nature (Lond.) 279, 773–777.CrossRefGoogle Scholar
  2. Buehner, M., Ford, G.C., Moras, D., Olsen, K.W. & Rossmann, M.G. (1974) J. Mol. Biol. 90, 25–49.PubMedCrossRefGoogle Scholar
  3. Campbell, J.W., Watson, H.C. & Hodgson, G.I. (1974) Nature 250, 301–303.PubMedCrossRefGoogle Scholar
  4. Fothergill, L.A. & Watson, H.C. (1989) in “Advances in Enzymology” (Meister, A. ed.), Vol.62, 227–313. Wiley: New York.Google Scholar
  5. Hol, W.G.J., van Duijnen, P.T. and Berendsen, H.J.C. (1978) Nature Lond., 273, 443–446.PubMedCrossRefGoogle Scholar
  6. Lesk, A.K. and Hardman, K.D. (1982) Science, 216, 539–540.PubMedCrossRefGoogle Scholar
  7. Leatherbarrow, R.J. and Fersht, A.R. (1986) Protein Engineering, 1, 7–16.PubMedCrossRefGoogle Scholar
  8. Levitt, M.R. and Chothia, C. (1976) Nature 261, 552–556.PubMedCrossRefGoogle Scholar
  9. Moras, D., Olsen, K.W., Sabesan, M.N., Buehner, M., Ford, G.C. and Rossmann, M.G. (1975) J. Biol. Chem., 250, 9137–9162.PubMedGoogle Scholar
  10. Mougin, A., Corbier, C., Soukri, A., Wonacott, C., Bralant, C. and Bralant, G. (1988) Protein Engineering, 2, 45–48.PubMedCrossRefGoogle Scholar
  11. Pizer, L.F. and Ballou, C.E. (1959) J. Biol. Chem., 234, 1138–1142.Google Scholar
  12. Reed, R., Fujinaga, M., Littlechild, J.A., and Watson, H.C. (1989) to be published.Google Scholar
  13. Rose, Z.B. (1982) in “Methods in Enzymology”, (Colowick, S.P. and Caplan, N.O. eds.) 87, 42–51. Academic Press: New York.Google Scholar
  14. Rossmann, M.G., Liljas, A., Branden, C.-I. and Banaszak, L. (1975) in “The Enzymes” (Boyer, P.D. ed.) Vol 2, 61–93. Academic Press: New York.Google Scholar
  15. Scopes, R.K. (1975) in “Methods in Enzymology”, (Colowick, S.P. and Caplan, N.O. eds.) 42, 134–137. Academic Press: New York.Google Scholar
  16. Skarzynski, T., Moody, P.C.E. & Wonacott, A.J. (1987) 193, 171–187.Google Scholar
  17. Trentham, D.R. (1971) Biochem. J., 122, 59–61.PubMedGoogle Scholar
  18. Warwicker, J. & Watson, H.C. (1982) J. Molec. Biol., 174, 671–679.Google Scholar
  19. Watson, H.C., Walker, N.P.C., Shaw, P.J., Bryant, T.N., Wendell, P.L., Fothergill, L.A., Perkins, R.E., Conroy, S.C., Dobson, M.J., Tuite, M.F., Kingsman, A.J., & Kingsman, S.M. (1982) E.M.B.O. J., 1, 1635–1640.Google Scholar
  20. White, M.F., and Fothergill,-Gilmore, L.A. (1988) FEBS Lett., 229, 383–387.PubMedCrossRefGoogle Scholar
  21. Wilson, C.A.B., Hardman, N., Fothergill-Gilmore, L.A., Gamblin, S.J. and Watson, H. (1987) Biochem. J., 241, 609–614.PubMedGoogle Scholar
  22. Winn, S.I., Watson, H.C., Harkins, R.N. & Fothergill, L.A. (1981) Phil. Trans. R. Soc. Lond. B, 293, 121–130.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Herman C. Watson
    • 1
  1. 1.Biochemistry Department School of Medical SciencesThe UniversityBristolEngland

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