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Methods for the Isolation and Structural Characterization of Hepatocyte Gap Junctions

  • Daniel A. Goodenough

Abstract

Electron microscopy has revealed several different kinds of morphologically distinguishable plasma membrane modifications which are involved in cell-cell interactions. In general, these specialized sites at the cell surface, collectively called intercellular junctions, may be divided into three broad functional categories: (1) those involved in intercellular adhesion, e.g., the desmosome, (2) those involved in regulating transepithelial permeability, e.g., the zonula occludens, and (3) those involved in intercellular communication, e.g., the chemical synapse and the gap junction.

Keywords

Tight Junction Intercellular Junction Hexagonal Array Liver Plasma Membrane Plasma Membrane Fraction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Barr, L., Dewey, M. M., and Berger, W., 1965, Propagation of action potentials and the structure of the nexus in cardiac muscle, J. Gen. Physiol. 48:797.PubMedCrossRefGoogle Scholar
  2. Barr, L., Berger, W., and Dewey, M. M., 1968, Electrical transmission at the nexus between smooth muscle cells, J. Gen Physiol. 51:347.PubMedCrossRefGoogle Scholar
  3. Benedetti, E. L., and Emmelot, P., 1965, Electron microscope observations on negatively stained plasma membranes isolated from rat liver, J. Cell Biol. 26:299.PubMedCrossRefGoogle Scholar
  4. Benedetti, E. L., and Emmelot, P., 1967, Studies on plasma membranes. IV. The ultrastructural localization of sialic acid in plasma membranes isolated from rat liver and hepatoma, J. Cell Sci. 2:499.PubMedGoogle Scholar
  5. Benedetti, E. L., and Emmelot, P., 1968a, Hexagonal array of subunits in tight junctions separated from isolated rat liver plasma membranes, J. Cell Biol. 38:15.PubMedCrossRefGoogle Scholar
  6. Benedetti, E. L., and Emmelot, P., 1968a, Structure and function of membranes isolated from liver, in: The Membranes (A. J. Dalton, and F. Haguenau, eds.), p. 33, Academic Press, New York.Google Scholar
  7. Benedetti, E. L., Dunia, I., and Diawara, A., 1973, The organization of the plasma membrane in mammalian cells, Eur. J. Cancer 9:263.PubMedCrossRefGoogle Scholar
  8. Berry, M. N., and Friend, D. S., 1969, High-yield preparation of isolated rat liver parenchymal cells: A biochemical and fine structural study, J. Bell Biol. 43:506.Google Scholar
  9. Bizzozero, G., 1870, Osservazioni sulla struttura degli epitheli pavimentose stratificati, Ren. Ist. Lombardo Sci. Lett. Ser. II 3:675.Google Scholar
  10. Borysenko, J. Z., and Revel, J. P., 1973, Experimental manipulation of desmosome structure, Am. J. Anat. 137:403.PubMedCrossRefGoogle Scholar
  11. Brown, P. K., 1972, Rhodopsin rotates in the visual receptor membrane, Nature New Biol. 236:35.PubMedGoogle Scholar
  12. Chalcroft, J. P., and Bullivant, S., 1970, An interpretation of liver cell membrane and junction structure based on observation of freeze-fracture replicas of both sides of the fracture, J. Cell Biol. 47:49.PubMedCrossRefGoogle Scholar
  13. Chambers, S., and Renyi, G. S., 1925, The structure of the cells in tissues as revealed by microdissection. I. The physical relationships of the cells in epithelia, Am. J. Anat. 35:385.CrossRefGoogle Scholar
  14. Claude, P., and Goodenough, D. A., 1973, Fracture faces of zonulae occludentes from “tight” and “leaky” epithelia, J. Cell Biol. 58:390.PubMedCrossRefGoogle Scholar
  15. Dewey, M. M., and Barr, L., 1962, Intercellular connections between smooth muscle cells: The nexus, Science (Wash.) 137:670.CrossRefGoogle Scholar
  16. Douglas, W. H. J., Ripley, R. C., and Ellis, R. A., 1970, Enzymatic digestion of desmosome and hemidesmosome plaques performed on ultra thin sections, J. Cell Biol. 44:211.PubMedCrossRefGoogle Scholar
  17. Eichholz, A., and Crane, R. K., 1965, Studies on the brush border in intestinal epithelial cells. I. Tris disruption of isolated hamster brush borders and density gradient separation of fractions, J. Cell Biol. 26:687.PubMedCrossRefGoogle Scholar
  18. Evans, W. H., and Gurd, J. W., 1972, Preparation and properties of nexus and lipidenriched vesicles from mouse liver plasma membranes, Biochem. J. 128:691.PubMedGoogle Scholar
  19. Fairbanks, G., Steck, T. L., and Wallach, D. F. H., 1971, Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane, Biochemistry 10:2606.PubMedCrossRefGoogle Scholar
  20. Farquhar, M. G., and Palade, G. E., 1963, Junctional complexes in various epithelia, J. Cell Biol 17:375.PubMedCrossRefGoogle Scholar
  21. Frömter, E., and Diamond, J., 1972, Route of passive ion permeation in epithelia, Nature New Biol. 235:9.PubMedCrossRefGoogle Scholar
  22. Gilula, N. B., 1971, Studies on the septate junction, Ph.D. thesis, University of California, Berkeley.Google Scholar
  23. Gilula, N. B., and Satir, P., 1971, Septate and gap junctions in molluscan gill epithelium, J. Cell Biol. 51:869.PubMedCrossRefGoogle Scholar
  24. Goodenough, D. A., 1970, The ultrastructure and biochemistry of hepatic intercellular junctions, Ph.D. thesis, Harvard University.Google Scholar
  25. Goodenough, D. A., 1974, Bulk isolation of hepatocyte gap junctions: Characterization of the principal protein, connexin, J. Cell Biol. 61:557–563.PubMedCrossRefGoogle Scholar
  26. Goodenough, D. A., and Gilula, N. B., 1972, Cell junctions and intercellular communication, in: Membranes and Viruses in Immunopathology (S. B. Day and R. A. Good, eds.), pp. 155–168, Academic Press, New York.Google Scholar
  27. Goodenough, D. A., and Gilula, N. B., 1974, The splitting of hepatocyte gap junctions and zonulae occludentes with hypertonic disaccharides, J. Cell Biol. 61:575–590.PubMedCrossRefGoogle Scholar
  28. Goodenough, D. A., and Revel, J. P., 1970, A fine structural analysis of intercellular junctions in the mouse liver, J. Cell Biol. 45:272.PubMedCrossRefGoogle Scholar
  29. Goodenough, D. A., and Revel, J. P., 1971, The permeability of isolated and in situ mouse hepatic gap junctions studied with enzymatic traces, J. Cell Biol. 50:81.PubMedCrossRefGoogle Scholar
  30. Goodenough, D. A., and Stoeckenius, W., 1972, Isolation of mouse hepatocyte gap junctions: Preliminary chemical characterization and x-ray diffraction, J. Cell Biol. 54:646.PubMedCrossRefGoogle Scholar
  31. Hand, A. R., and Gobel, S., 1972, The structural organization of the septate and gap junctions of Hydra, J. Cell Biol. 52:397.PubMedCrossRefGoogle Scholar
  32. Harrison, S. C., David, A., Jumblatt, J., and J. E. Darnell, 1971a, Lipid and protein organization in Sindbis virus, J. Mol. Biol. 60:523.CrossRefGoogle Scholar
  33. Harrison, S. C., Caspar, D. L. D., Camerini-Otero, R. D., and Franklin, R. M., 1971b, Lipid and protein arrangement in bacteriophage PM2, Nature New Biol. 229:197.PubMedGoogle Scholar
  34. Jones, R. B., Staton, A. J., and Kiesow, L. A., 1973, Isolation of rat liver membranes by zonal centrifugation: Increased yield with Ca++, Anal. Biochem. 55:154.PubMedCrossRefGoogle Scholar
  35. Karnovsky, M. J., 1965, A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy, J. Cell Biol. 27:137a.Google Scholar
  36. Kelly, D. E., 1966, Fine structure of desmosomes, hemidesmosomes, and an adepidermal globular layer in developing new epidermis, J. Cell Biol. 28:51.PubMedCrossRefGoogle Scholar
  37. Klug, A., and Berger, J. E., 1964, An optical method for the analysis of periodicities in electron micrographs, and some observations on the mechanism of negative staining, J. Mol. Biol. 10:565.PubMedCrossRefGoogle Scholar
  38. Klug, A., and DeRosier, D. J., 1966, Optical filtering of electron micrographs: Reconstruction of one-sided images, Nature (Lond.) 212:29.PubMedCrossRefGoogle Scholar
  39. Kreutziger, G. O., 1968, Freeze-etching of intercellular junctions of mouse liver, Proc. Electron Microsc. Soc. Am. 26:234.Google Scholar
  40. Leeson, T. S., and Kalant, H., 1961, Effects of in vivo decalcification on ultrastructure of adult rat liver, J. Biophys. Biochem. Cytol. 10:95.PubMedCrossRefGoogle Scholar
  41. Loewenstein, W. R., 1967, On the genesis of cellular communication, Develop. Biol. 15:503.PubMedCrossRefGoogle Scholar
  42. McNutt, N. S., and Weinstein, R. S., 1973, Membrane ultrastructure at mammalian intercellular junctions, Prog. Biophys. Mol. Biol. 26:45.PubMedCrossRefGoogle Scholar
  43. Miller, F., 1960, Hemoglobin absorption by the cells of the proximal convoluted tubule in the mouse kidney, J. Biophys. Biochem. Cytol. 8:689.PubMedCrossRefGoogle Scholar
  44. Neville, D. M., 1960, The isolation of a cell membrane fraction from rat liver, J. Biophys. Biochem. Cytol. 8:413.PubMedCrossRefGoogle Scholar
  45. Nigam, V. N., Morais, R., and Karasaki, S., 1971, A simple method for the isolation of rat liver cell plasma membranes in isotonic sucrose, Biochim. Biophys. Acta 249:34.PubMedCrossRefGoogle Scholar
  46. Odland, G. F., 1958, The fine structure of the interrelationship of cells in the human epidermis, J. Biophys. Biochem. Cytol. 4:529.PubMedCrossRefGoogle Scholar
  47. Overton, J., 1968, The fate of desmosomes in trypsinized tissue, J. Exp. Zool. 168:203.PubMedCrossRefGoogle Scholar
  48. Overton, J., Eichholz, A., and Crane, R. K., 1965, Studies on the organization of intestinal epithelial cells. II. Fine structure of fractions of tris-disrupted hamster brush borders, J. Cell Biol. 26:693.PubMedCrossRefGoogle Scholar
  49. Pinto da Silva, P., and Branton, D., 1970, Membrane splitting in freeze-etching: Covalently bound ferritin as a membrane marker, J. Cell Biol. 45:598.CrossRefGoogle Scholar
  50. Porter, K. R., 1954, Observations on the submicroscopic structure of animal epidermis, Anat. Rec. 118:433.Google Scholar
  51. Rambourg, A., and Leblond, C. P., 1967, Electron microscope observations on the carbohydrate-rich cell coat present at the surface of cells in the rat, J. Cell Biol. 32:27.PubMedCrossRefGoogle Scholar
  52. Rash, J. E., Shay, J. W., and Biesele, J. J., 1968, Urea extractions of z-bands, intercalated discs, and desmosomes, J. Ultrastruct. Res. 24:181.PubMedCrossRefGoogle Scholar
  53. Ray, T. K., 1970, A modified method for the isolation of the plasma membrane from rat liver, Biochim. Biophys. Acta 196:1.PubMedCrossRefGoogle Scholar
  54. Revel, J. P., and Karnovsky, M. J., 1967, Hexagonal array of subunits in intercellular junctions of the mouse heart and liver, J. Cell Biol. 33:C7.PubMedCrossRefGoogle Scholar
  55. Robertson, J. D., 1963, The occurrence of a subunit pattern in the unit membranes of club endings in Mauthner cell synapses in goldfish brains, J. Cell Biol. 19:201.PubMedCrossRefGoogle Scholar
  56. Satir, P., and Gilula, N. B., 1973, The fine structure of membranes and intercellular communication in insects, Ann. Rev. Entomol. 18:143.CrossRefGoogle Scholar
  57. Schaffer, J., 1927, Epithelgewebe in: Handbuch der mikroskopischen Anatomie des Menschen, Vol. 2(1) (W. von Mollendorff, ed.), p. 35, Verlag von Julius Springer, Berlin.Google Scholar
  58. Selby, C. C., 1955, An electron microscopic study of the epidermis of mammalian skin in thin sections, J. Biophys. Biochem. Cytol. 1:429.PubMedCrossRefGoogle Scholar
  59. Staehelin, L. A., 1974, Structure and function of intercellular junctions, Ann. Rev. Cytol. (in press).Google Scholar
  60. Staehelin, L. A., Mukherjee, T. M., and Williams, A. W., 1969, Freeze-etch appearance of the tight junctions in the epithelium of small and large intestine of mice, Protoplasma 67:165.PubMedCrossRefGoogle Scholar
  61. Wade, J. B., and Karnovsky, M. J., 1974, The structure of the zonula occludens: A single fibril model based on freeze-fracture, J. Cell Biol. 60:168.PubMedCrossRefGoogle Scholar
  62. Wilkins, M. F. H., Blaurock, A. E., and Engleman, D. M., 1971, Bilayer structure in membranes, Nature New Biol. 230:72.PubMedCrossRefGoogle Scholar
  63. Zampighi, G., and Robertson, J. D., 1973, The fine structure of synaptic discs separated from the goldfish medulla oblongata, J. Cell Biol. 56:92.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • Daniel A. Goodenough
    • 1
  1. 1.Department of AnatomyHarvard Medical SchoolBostonUSA

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