Methods in Membrane Biology

Volume 3 Plasma Membranes

  • Edward D. Korn

Table of contents

  1. Front Matter
    Pages i-xvi
  2. C. Ronald Kahn
    Pages 81-146
  3. Nathan Sharon, Halina Lis
    Pages 147-200
  4. Robert T. Schimke
    Pages 201-236
  5. Back Matter
    Pages 237-246

About this book

Introduction

Volume 3 continues the approach carried out in the first two volumes of this se ries of publishing articles on membrane methodology which include, in addition to procedural details, incisive discussions of the ap­ plications of the methods and of their limitations. Wh at is the theoretical basis of the method, how and to what problems can it be applied, how does one interpret the results, what has thus far been achieved by the method, what lies in the future-these are the questions the authors have tried to answer. No area of membrane biology engages the interest of more investigators than studies of the plasma membrane. Four chapters in this volume are concerned with one or more aspects of the cell surface. Fundamental to all studies of the cell surface are the isolation and characterization of pure plasma membranes. Many preparations described in the literature are inadequate or are inadequately characterized. In the first chapter, Neville discusses the theoretical and practical bases of tissue fractionation, empha­ sizes the variations in enzyme content among plasma membranes from different sources, offers guidance in the choice of the proper criteria for assessing membrane purity, and suggests the best markers for detecting the possible presence of contaminating organelles. To review in detail each of the many preparations of plasma membranes that have been published is impossible.

Keywords

Glycogen Plasma biology cell enzyme enzymes membrane membrane biology mitochondria plasma membrane protein receptor tissue

Editors and affiliations

  • Edward D. Korn
    • 1
  1. 1.Laboratory of Cell BiologyNational Heart and Lung InstituteBethesdaUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4757-0265-1
  • Copyright Information Springer-Verlag US 1975
  • Publisher Name Springer, Boston, MA
  • eBook Packages Springer Book Archive
  • Print ISBN 978-1-4757-0267-5
  • Online ISBN 978-1-4757-0265-1
  • About this book