Advertisement

Initiation of DNA Synthesis on Single-Stranded DNA Templates in Vitro Promoted by the Bacteriophage λ O and P Replication Proteins

  • Jonathan H. LeBowitz
  • Roger McMacken
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 179)

Abstract

The bacteriophage λ O and P protein replication initiators, in conjunction with six purified Escherichia coli replication proteins, replicate the single-stranded chromosomes of phages M13 and ΦX174 to a duplex form. Several discrete steps are involved in this DNA synthesis reaction. In an ATP-dependent step that preceeds priming, the λ O and P proteins interact with the Escherichia coli dnaJ and dnaK proteins to transfer the bacterial dnaB protein onto DNA coated with single-stranded DNA binding protein. This creates a stable prepriming intermediate, isolable by gel filtration, that is rapidly primed and replicated upon the addition of primase and DNA polymerase III holoenzyme. Each of the eight proteins required for this nonspecific single strand replication reaction also have physiological roles in the replication of the bacteriophage λ chromosome in vivo. We propose a scheme for the λ O and P protein-dependent initiation of DNA synthesis that may be relevant to strand initiation events occurring during λ DNA replication.

Keywords

Duplex Form Protein Replication Initiator dnaJ Protein dnaK Protein Replication Reaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Skalka, A. (1977) Curr. Top, Microbiol. Immunol. 78, 201.CrossRefGoogle Scholar
  2. 2.
    Furth, M.E. and Wickner, S.H., Lambda DNA replication (1983) in: “Lambda II”, Hendrix, R.W., Roberts, J.W., Stahl, F.W. and Weisberg, R.A., eds., Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.Google Scholar
  3. 3.
    Kornberg, A. (1980) “DNA Replication”, Freeman, W.H., San Francisco.Google Scholar
  4. 4.
    Kornberg, A. (1982) “1982 Supplement to DNA Replication”, Freeman, W.H., San Francisco.Google Scholar
  5. 5.
    Nossal, N.G. (1983) Ann. Rev. Biochem. 52, 581.PubMedCrossRefGoogle Scholar
  6. 6.
    Wold, M.S., Mallory, J.B., Roberts, J.D., LeBowitz, J.H. and McMacken, R. (1982) Proc. Natl. Acad. Sci. USA 79, 6176.PubMedCrossRefGoogle Scholar
  7. 7.
    Tsurimoto, T., Hase, T. Matsubara, H. and Matsubara, K. (1982) Molec. Gen. Genet. 187, 79.PubMedCrossRefGoogle Scholar
  8. 8.
    Roberts, J.D. and McMacken, R. (1983) Nucleic Acids Res. 11, 7435.PubMedCrossRefGoogle Scholar
  9. 9.
    Mallory, J.B. (1983) Ph. D. dissertation, The Johns Hopkins University, Baltimore, Maryland.Google Scholar
  10. 10.
    Tsurimoto, T. and Matsubara, K. (1981) Nucleic Acids Res. 9, 1789.PubMedCrossRefGoogle Scholar
  11. 11.
    Klein, A., Lanka, E. and Schuster, H. (1980) Eur. J. Biochem. 105, 1.PubMedCrossRefGoogle Scholar
  12. 12.
    McMacken, R., Wold, M.S., LeBowitz, J.H., Roberts, J.D., Mallory, J.B., Wilkinson, A.K. and Loehrlein, C. (1983) in: “Mechanisms of DNA Replication and Recombination”, Cozzarelli, N.R. ed., UCLA Symposia on Molecular and Cellular Biology, New Series, Vol. 10, Alan R. Liss, New York, 819.Google Scholar
  13. 13.
    Zylicz, M., LeBowitz, J.H., McMacken, R. and Georgopoulos, C. (1983) Proc. Natl. Acad. Sci. USA 80, 6431.PubMedCrossRefGoogle Scholar
  14. 14.
    Anderl, A. and Klein, A. (1982) Nucleic Acids Res. 10, 1733.PubMedCrossRefGoogle Scholar
  15. 15.
    Tsurimoto, T. and Matsubara, K. (1982) Proc. Natl. Acad. Sci. USA 79, 7639.PubMedCrossRefGoogle Scholar
  16. 16.
    Ray, D.S., Hines, J.C., Kim, M.H., Imber, R. and Nomura, N. (1982) Gene 18, 231.PubMedCrossRefGoogle Scholar
  17. 17.
    LeBowitz, J.H. and McMacken, R. (1984), submitted for publication.Google Scholar
  18. 18.
    McMacken, R., Ueda, K. and Kornberg, A. (1977) Proc. Natl. Acad. Sci. USA 74, 4190.PubMedCrossRefGoogle Scholar
  19. 19.
    Arai, K. and Kornberg, A. (1981) Proc. Natl. Acad. Sci. USA 78, 69.PubMedCrossRefGoogle Scholar
  20. 20.
    Weiner, J.H., McMacken, R. and Kornberg, A. (1976) Proc. Natl. Acad. Sci. USA 73, 752.PubMedCrossRefGoogle Scholar
  21. 21.
    Arai, R., Low, R., Robori, J., Shlomai, J. and Kornberg, A. (1981) J. Biol. Chem. 256, 5273.PubMedGoogle Scholar
  22. 22.
    Shlomai, J. and Kornberg, A. (1980) Proc. Natl. Acad. Sci. USA 77, 799.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Jonathan H. LeBowitz
    • 1
  • Roger McMacken
    • 1
  1. 1.Department of Biochemistry, School of Hygiene and Public HealthThe Johns Hopkins UniversityBaltimoreUSA

Personalised recommendations