DNA Polymerase III Holoenzyme of Escherichia Coli: An Asymmetric Dimeric Replicative Complex Containing Distinguishable Leading and Lagging Strand Polymerases
The DNA polymerase III holoenzyme is the major replicative enzyme in Escherichia coli (for a review see ref. 1). A final definition of the enzyme’s structure has not yet been accomplished, but it is apparent that it contains at least seven different subunits: α, ε, θ, β, δ, γ and τ (2–4). The holoenzyme contains a catalytic core, termed DNA polymerase III, composed of the α, ε and θ subunits. DNA polymerase III can catalyze limited synthesis in short gaps created by nuclease treatment of duplex DNA, but it is inactive in reconstituted natural Escherichia coli replication systems (3, 5).
KeywordsReplication Fork Initiation Complex Functional Asymmetry Okazaki Fragment Final Definition
Unable to display preview. Download preview PDF.
- 1.McHenry, C.S. and Kornberg, A. (1982) in: The Enzymes, P. Boyer, ed., Academic Press, New York.Google Scholar
- 7.Kornberg, A. (1982) DNA Replication, Supplement, W.H. Freeman and Co., San Francisco.Google Scholar
- 14.Johanson, K. and McHenry, C.S. (1984) submitted for publication.Google Scholar
- 15.Hübscher, U. and Ottiger, H.P. (1984) this volume.Google Scholar