Summary
We describe the mapping of several genetic loci involved in the replication of the pSClOl plasmid. These include the origin of replication and a short segment of DNA that encodes a pSClOl incompatibility function. This short segment lies within the origin region. Flanking the incompatibility segment are two loci, repA and repB, which are required for replication. The product of the repA locus is shown to be trans-acting.
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References
Cohen, S. N. and Chang, A. C. Y. (1973) Proc. Natl. Acad. Sci. USA 70, 1293.
Cohen, S. N. and Chang, A. C. Y. (1977) J. Bacteriol. 132, 734.
Cabello, F., Timmis, K. and Cohen, S. N. (1976) Nature 259, 285.
Frey, J., Chandler, M. and Caro, L. (1979) Mol. Gen. Genet. 174, 117.
Hasunuma, K. and Sekiguchi, M. (1977) Mol. Gen. Genet. 154, 225.
Hirota, Y, Ryter, A. and Jacob, F. (1968) Cold Spring Harbor Symp. Quant. Biol. XXXIII, 677.
Fuller, R. S., Kaguni, J. M. and Kornberg, A. (1981) Proc. Natl. Acad. Sci. USA 78, 7370.
Linder, P., Churchward, G. and Caro, L. (1983) J. Mol. Biol. 170, 287.
Churchward, G., Linder, P. and Caro, L. (1983) Nucl. Acids Res. 11, 5645.
Guyer, M. (1978) J. Mol. Biol. 126, 347.
Meacock, P. A. and Cohen, S. N. (1980) Cell 20, 529.
Casadaban, M. J., Chou, J. and Cohen, S. N. (1980) J. Bacteriol. 143, 971.
Pannekoek, H., Maat, J., Berg, E. rd. and Nordermeer, I. (1980) Nucl. Acids Res. 8, 1535.
Stalker, D. M., Thomas, C. M. and Helinski, D. R. (1981) Mol. Gen. Genet. 181, 8.
Stalker, P. M., Kolter, R. and Helinski, D. R. (1982) J. Mol. Biol. 161, 33.
Murotsu, T., Matsubara, K., Sugisachi, H. and Takanami, M. (1981) Gene 15, 257.
Kolter, R. and Helinski, D. R. (1982) J. Mol. Biol. 161, 45.
Thomas, C. M., Meyer, R. and Helinski, D. R. (1980) J. Bacteriol. 141, 213.
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© 1984 Springer Science+Business Media New York
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Churchward, G., Linder, P., Caro, L. (1984). Replication Functions Encoded by the Plasmid pSClOl. In: Proteins Involved in DNA Replication. Advances in Experimental Medicine and Biology, vol 179. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8730-5_21
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DOI: https://doi.org/10.1007/978-1-4684-8730-5_21
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-8732-9
Online ISBN: 978-1-4684-8730-5
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