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Participation of Hepatic Parenchymal and Kupffer Cells in Chylomicron and Cholesterol Metabolism

  • N. R. Di Luzio
  • S. J. Riggi
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1)

Abstract

The administration of reticuloendothelial (RE) stimulants produced an increased growth and functional activity of RE cells. Parenchymal cell number, structure, and function, as indicated by BSP removal, and histological and electron-microscopic observations were unaltered in RE-stimulated rats. The RE hyperactive group manifested an increased intravascular removal of colloidal carbon which was associated with enhanced accumulation of carbon in Kupffer cells.

The technique of selectively and exclusively producing an RE hyper-functional state was employed to evaluate the metabolic behavior of labeled chyle, chylomicrons, and an artificial lipid emulsion, designated as “RE test lipid emulsion.” The intravascular removal of chyle and chylomicrons was not enhanced in RE-stimulated rats. The predominant uptake of injected chyle and chylomicrons was hepatic in nature with lung and spleen manifesting 1-3% removal. Cholesterol-containing chylomicrons demonstrated a behavior similar to that of triglyceride-containing chylomicrons and unlike colloidal carbon and the RE test lipid emulsion. Significant differences existed, however, between the organ uptake of cholesterol and triglyceride-labeled chylomicrons.

An artificial lipid emulsion was developed that possessed an intravascular behavior and an organ distribution which was comparable to colloidal and particulate materials which are removed by phagocytosis. This RE test lipid emulsion has been employed in the evaluation of RE function in experimental and clinical subjects.

Normal chylomicrons containing cholesterol or triglyceride are relatively immune to RE cell uptake and the hepatic mechanism of chylomicron removal involves essentially their removal by the parenchymal cell.

Keywords

Parenchymal Cell Kupffer Cell Cholesterol Metabolism Lipid Emulsion Liver Uptake 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    S. H. Gage, Cornell Vet., 10: 154, 1920.Google Scholar
  2. 2.
    G. Gulliver, Gerber’s General Anatomy, 1847, p. 88.Google Scholar
  3. 3.
    S. H. Gage and P.A. Fish, Am. J. Anat., 34: 1, 1924.CrossRefGoogle Scholar
  4. 4.
    V.P. Dole and J.T. Hamlin, III, Physiol. Rev., 42: 674, 1962.PubMedGoogle Scholar
  5. 5.
    T. Olivecrona, E.P. George, and B. Borgstrom, Federation Proc., 20: 928, 1961.Google Scholar
  6. 6.
    R.E. Olson and J. W. Vester, Physiol. Rev., 40: 677, 1960.PubMedGoogle Scholar
  7. 7.
    B. Borgstrom and N. Tryding, Acta Physiol. Scand., 37: 127, 1956.PubMedCrossRefGoogle Scholar
  8. 8.
    R.J. Havel, Am. J. Clin. Nutr., 6: 662, 1958.PubMedGoogle Scholar
  9. 9.
    C.B. Laurell, Scand. J. Clin. Lab. Invest., 6: 22, 1954.PubMedCrossRefGoogle Scholar
  10. 10.
    B. Borgstrom, in: K. Bloch, Ed., Lipide Metabolism. New York, John Wiley and Sons, 1960, p. 128.Google Scholar
  11. 11.
    J. E. French and B. Morris, J. Physiol., 138: 326, 1957.PubMedGoogle Scholar
  12. 12.
    R.J. Havel and D. S. Fredrickson, J.Clin.Invest., 35: 1025, 1956.PubMedCrossRefGoogle Scholar
  13. 13.
    J.E. French and B. Morris, J. Physiol., 140: 262, 1958.PubMedGoogle Scholar
  14. 14.
    B. Borgstrom, P. Wlodawer, and C. Naito, Digestion, Absorption Intestinale et Transport des Glycerides Chez Les Animaux Superieurs. Paris, Centre National de la Recherche Scientifique, 1961, p. 125.Google Scholar
  15. 15.
    T. Olivecrona, J. Lipid Res., 3: 439, 1962.Google Scholar
  16. 16.
    T. Olivecrona, Acta Physiol. Scand., 55: 170, 1962.PubMedCrossRefGoogle Scholar
  17. 17.
    J. H. Bragdon and R.S. Gordon, J. Clin. Invest., 37: 574, 1958.PubMedCrossRefGoogle Scholar
  18. 18.
    R. J. Havel and A. Golfien, J. Lipid Res., 2: 389, 1961.Google Scholar
  19. 19.
    P. Belfrage, B. Borgstrom, and T. Olivecrona, Acta Physiol. Scand., 58: 111, 1963.PubMedCrossRefGoogle Scholar
  20. 20.
    D.S. Goodman, J. Clin.Invest., 41: 1886, 1962.PubMedCrossRefGoogle Scholar
  21. 21.
    R. Daoust: R.W. Brauer, Ed., Liver Function. Washington, D. C., Am. Inst. Biol. Sciences, 1958, p. 3.Google Scholar
  22. 22.
    R.H. Jaffe and S. L. Berman, Arch. Pathol. Lab. Med., 5: 1020, 1928.Google Scholar
  23. 23.
    R. H. Jaffe, in: H. Downey, Ed., Handbook of Hematology. New York, P.B. Hoeber, 1938, p. 977.Google Scholar
  24. 24.
    M. Friedman, S.O. Byers, and S. St. George, Am.J.Physiol., 184: 141, 1956.PubMedGoogle Scholar
  25. 25.
    S.O. Byers, S. St. George, and M. Friedman, in: B.N. Halpern, Ed., Physiopathology of the Reticuloendothelial System. Springfield, Illinois, Charles C. Thomas, 1957, p. 128.Google Scholar
  26. 26.
    T. Neveu, G. Biozzi, B. Benacerraf, C. Stiffel, and B.N. Halpern, Am.J.Physiol., 187: 269, 1956.PubMedGoogle Scholar
  27. 27.
    S.J. Thannhauser, Lipidoses. New York, Oxford University Press, 1950.Google Scholar
  28. 28.
    N.R. DiLuzio, Ann.N.Y.Acad.Sci., 88: 244, 1960.Google Scholar
  29. 29.
    N.R. DiLuzio, Nature, 185: 616, 1960.CrossRefGoogle Scholar
  30. 30.
    N.R. DiLuzio, J. Houston, and E.E. Elko, in: S. Garattini and R. Paoletti, Eds., Drugs Affecting Lipid Metabolism. Amsterdam, Elsevier Publishing Co., 1961, p. 228.Google Scholar
  31. 31.
    S.J. Riggi and N.R. DiLuzio, J. Lipid Res., 3: 339, 1962.Google Scholar
  32. 32.
    N. R. DiLuzio and S.J. Riggi, J. Reticuloendothelial Soc., 3: 135, 1966.Google Scholar
  33. 33.
    W.R. Waddell, B.P. Geyer, E. Clarke, and F.J. Stare, Am.J. Physiol., 177: 90, 1954.PubMedGoogle Scholar
  34. 34.
    R.G. Murray and S. Freeman, J. Lab. Clin. Med., 38: 56, 1951.PubMedGoogle Scholar
  35. 35.
    J. Van Den Bosch, E. Evrard, A. Billiau, J.V. Joossens, and P. DeSomer, J. Exptl. Med., 114: 1035, 1961.CrossRefGoogle Scholar
  36. 36.
    C. T. Ashworth, V.A. Stembridge, and E. Sanders, Am. J. Physiol., 198: 1326, 1960.PubMedGoogle Scholar
  37. 37.
    C. T. Ashworth, N. R. DiLuzio, and S.J. Riggi, Exptl. Mol. Pathol. Suppl., 1: 83, 1963.Google Scholar
  38. 38.
    G. Biozzi, B. Bencerraf, and B. N. Halpern, Brit. J. Exptl. Pathol., 34: 441, 1953.Google Scholar
  39. 39.
    N.R. DiLuzio, J.Am.Oil Chemists’ Soc., 37: 163, 1960.CrossRefGoogle Scholar
  40. 40.
    N. R. DiLuzio, K.A. Simon, and A. C. Upton, Arch. Pathol., 64: 649, 1957.Google Scholar
  41. 41.
    D.A. Blickens and N.R. DiLuzio, J. Reticuloendothelial Soc., 1: 68, 1964.Google Scholar
  42. 42.
    B. Benacerraf and M.M. Sebestyen, Federation Proc., 16: 860, 1957.Google Scholar
  43. 43.
    L.S. Kelly, E.L. Dobson, C.R. Finney, and J.D. Hirsch, Am.J. Physiol., 198: 1134, 1960.PubMedGoogle Scholar
  44. 44.
    S.J. Riggi and N.R. DiLuzio, Am.J.Physiol., 200: 297, 1961.PubMedGoogle Scholar
  45. 45.
    S.J. Riggi and N.R. DiLuzio, Nature, 193: 1292, 1962.PubMedCrossRefGoogle Scholar
  46. 46.
    W.R. Wooles and N.R. DiLuzio, J. Reticuloendothelial Soc., 1: 160, 1941.Google Scholar
  47. 47.
    J.L. Bollman, J.C. Cain, and J.H. Grindlay, J. Lab. Clin. Med., 33: 1349, 1948.PubMedGoogle Scholar
  48. 48.
    N.R. DiLuzio and S.J. Riggi, J. Reticuloendothelial Soc., 1: 248, 1964.Google Scholar
  49. 49.
    N.K. Salky, N.R. DiLuzio, D.P. P’Pool, and A.J. Sutherland, J. Am. Med. Ass., 187: 744, 1964.CrossRefGoogle Scholar
  50. 50.
    N.R. DiLuzio, N.K. Salky, S.J. Riggi, and A.J. Ladman, in: The Reticuloendothelial System, Morphology, Immunology and Regulation. Kyoto, Japan, “Nissba” Printing Co., 1965, p. 389.Google Scholar
  51. 51.
    N.R. DiLuzio and S.J. Riggi, J. Reticuloendothelial Soc., 1: 136, 1964.Google Scholar
  52. 52.
    V. Aberdeen, P.A. Shepherd, and W.J. Simmonds, Quart. J. Exptl. Physiol., 45: 265, 1960.Google Scholar
  53. 53.
    I. Toro, P. Ruzsa, and P. Rohlich, Exptl. Cell Res., 26: 601, 1962.PubMedCrossRefGoogle Scholar
  54. 54.
    M. Kojima and Y. Imai, Proc. Japan Soc. Reticuloendothelial System, 1: 61, 1961.Google Scholar
  55. 55.
    W.J. Lossow, N. Brot, and I. L. Chaikoff, J. Lipid Res., 3: 207, 1962.Google Scholar
  56. 56.
    C. Markowitz and F.C. Mann, Am.J.Physiol., 93: 521, 1930.Google Scholar
  57. 57.
    Y. Stein and B. Shapiro, J. Lipid Res., 1: 326, 1960.PubMedGoogle Scholar
  58. 58.
    D. L. Berliner and T. F. Dougherty, in: J. H. Heller, Ed., Reticuloendothelial Structure and Function. New York, Ronald Press Co., 1960, p. 403.Google Scholar
  59. 59.
    T. F. Dougherty and D. L. Berliner, in: G. Pincus, Ed., Conference on Hormones and Atherosclerosis. New York, Academic Press, Inc., 1959, p. 103.Google Scholar
  60. 60.
    R. Jones, W.A. Thomas, and R. F. Scott, J. Exptl. and Mol. Pathol., 1: 65, 1962.CrossRefGoogle Scholar
  61. 61.
    P.J. Nestel and R.O. Scow, J. Lipid Res., 5: 46, 1964.Google Scholar
  62. 62.
    E. L. Bierman, E. Gordis, and J. T. Hamlin, III, J. Clin. Invest., 41 2254, 1962.PubMedCrossRefGoogle Scholar
  63. 63.
    N. R. DiLuzio and E. L. Bierman, Proc. Soc. Exptl. Biol. and Med., 116: 1045, 1964.Google Scholar
  64. 64.
    B. Morris and J. E. French, Quart.J. Exptl. Physiol., 43: 180, 1958.Google Scholar
  65. 65.
    B. Edgren and B. Ivemark, Acta Physiol.Scand., 48: 402, 1960.PubMedCrossRefGoogle Scholar
  66. 66.
    P. Belfrage, B. Edgren, and T. Olivecrona, Acta Physiol. Scand., 62: 344, 1964.PubMedCrossRefGoogle Scholar
  67. 67.
    B. Morris and F.C. Courtice, Quart. J. Exptl. Physiol., 41: 341, 1956.Google Scholar
  68. 68.
    R. Reiser, M.C. Williams, and M. F. Sorrels, J. Lipid Res., 1: 241, 1960.PubMedGoogle Scholar
  69. 69.
    B. Borgstrom and P. Jordan, Acta Soc. Med. Upsalien., 64: 185, 1959.Google Scholar
  70. 70.
    D. S. Fredrickson, D. L. McCollester, and K. Ono, J. Clin.Invest., 37: 1333, 1958.PubMedCrossRefGoogle Scholar
  71. 71.
    B. Shapiro, Digestion, Absorption Intestinale et Transport des Glycerides Chez Les Animaux Superieurs. Paris, Centre National de la Recherche Scientifique, 1961, p. 165.Google Scholar
  72. 72.
    D.W. Fawcett, J. Nat. Cancer Inst., 15: 1475, 1955.PubMedGoogle Scholar
  73. 73.
    J. C. Hampton, Texas Rep.Biol. and Med., 18: 602, 1960.Google Scholar
  74. 74.
    G. Holle, German Med. Monthly, 7: 91, 1962.Google Scholar
  75. 75.
    J.M. Felts and P.A. Mayes, Nature, 206: 195, 1965.PubMedCrossRefGoogle Scholar
  76. 76.
    J.M. Felts, Ann. N. Y. Acad. Sci., 131: 24, 1965.PubMedCrossRefGoogle Scholar
  77. 77.
    J.A. Higgins and C. Green, Biochem.J., 99: 631, 1966.PubMedGoogle Scholar
  78. 78.
    N.K. Salky, D. Mills, and N.E. DiLuzio, J. Lab. and Clin. Med., 66: 952, 1965.Google Scholar

Copyright information

© Springer Science+Business Media New York 1967

Authors and Affiliations

  • N. R. Di Luzio
    • 1
  • S. J. Riggi
    • 1
  1. 1.Department of Physiology and BiophysicsUniversity of Tennessee Medical UnitsMemphisUSA

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