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Long-Term Synergistic Regulation of Ionic Channels by C-Kinase and Ca2+/CaM-Type II Kinase

  • Chapter
Molecular Mechanisms of Neuronal Responsiveness

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 221))

Abstract

The distinct molecular identities of ionic channels within biological membranes are now being revealed. The acetylcholine receptor, a ligandgated cation channel, was solubilized and purified from post-synaptic membranes of the ray Torpedo californica (Karlin, 1980; Changeux, 1981). Entire amino acid sequences for all of the acetylcholine receptor subunits were deduced from DNA sequence analysis of cDNA clones (Numa et al., 1983). Similarly, the voltage-sensitive Na+ channel from rat brain has been solubilized and purified to homogeneity, and shown to consist of α (Mr 260,000), β1 and β2(Mr 39,000 and 37,000, respectively) subunits (Agnew et al., 1980; Weigele and Barchi, 1982; Hartshorne and Catterall, 1984). Consistent with the heterogeneity of tetrodotoxin binding sites, presence of at least three distinct Na+ channels (I, II and III) in rat brain was suggested from a sequencing study of cDNA clones obtained from three distinct mRNAs for the α-subunit (Noda et al., 1986). The Ca2+ channel (a dihydropyridine-sensitive class) was also purified (Curtis and Catterall, 1985). None of the known K+ channels have yet been purified, probably due to the unavailability of high affinity neurotoxins and a K+ channel abundance which is small in comparison to that of the Na+ channel and the acetylcholine receptor.

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Authors and Affiliations

  1. Section on Neural Systems, Laboratory of Biophysics, National Institute of Neurological and Communicative Disorders and Stroke, National Institutes of Health at the Marine Biological Laboratory, Woods Hole, Massachusetts, USA

    Daniel L. Alkon & Shigetaka Naito

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  1. Daniel L. Alkon
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  2. Shigetaka Naito
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Editors and Affiliations

  1. The University of Vermont College of Medicine, Burlington, Vermont, USA

    Yigal H. Ehrlich , Robert H. Lenox  & Elizabeth Kornecki ,  & 

  2. Bolling A.F. Base, U.S. Air Force Office of Scientific Research, Washington, D.C., USA

    William O. Berry

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© 1987 Plenum Press, New York

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Alkon, D.L., Naito, S. (1987). Long-Term Synergistic Regulation of Ionic Channels by C-Kinase and Ca2+/CaM-Type II Kinase. In: Ehrlich, Y.H., Lenox, R.H., Kornecki, E., Berry, W.O. (eds) Molecular Mechanisms of Neuronal Responsiveness. Advances in Experimental Medicine and Biology, vol 221. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7618-7_20

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  • DOI: https://doi.org/10.1007/978-1-4684-7618-7_20

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