Studies of the Autoprocessing of the HIV-1 Protease Using Cleavage Site Mutants

  • John M. Louis
  • Stephen Oroszlan
  • Peter T. Mora
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 306)


The distinguishing features of aspartyl proteases in retroviruses, vis à vis the semi-symmetrical aspartyl proteases in other organisms, is the paucity of the genetic information in the viruses sufficient for the synthesis of a monomelic protein, from which the homodimer is assembled with the formation of the characteristic active site structure.1


Fusion Protein Aspartyl Protease Active Site Structure Urea Denaturation Refold Procedure 
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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • John M. Louis
    • 1
  • Stephen Oroszlan
    • 2
  • Peter T. Mora
    • 3
  1. 1.Laboratory of Cellular and Developmental BiologyNIDDK, NIHBethesdaUSA
  2. 2.Laboratory of Molecular Virology and Carcinogenesis, ABL-Basic Research ProgramNCI-FCRDCFrederickUSA
  3. 3.Office of the DirectorDCBD, NCI, NIHBethesdaUSA

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