Advertisement

Studies of the Autoprocessing of the HIV-1 Protease Using Cleavage Site Mutants

  • John M. Louis
  • Stephen Oroszlan
  • Peter T. Mora
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 306)

Abstract

The distinguishing features of aspartyl proteases in retroviruses, vis à vis the semi-symmetrical aspartyl proteases in other organisms, is the paucity of the genetic information in the viruses sufficient for the synthesis of a monomelic protein, from which the homodimer is assembled with the formation of the characteristic active site structure.1

Keywords

Fusion Protein Aspartyl Protease Active Site Structure Urea Denaturation Refold Procedure 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    A. Wlodawer, M. Miller, M. Jaskólski, B. K. Sathyanarayana, E. Baldwin, I. T. Weber, L. M. Selk, L. Clawson, J. Schneider and S. B. H. Kent, Conserved folding in retroviral proteases: crystal structure of a synthetic HIV-1 protease, Science 245: 616 (1989).PubMedCrossRefGoogle Scholar
  2. 2.
    J. M. Louis, E. M. Wondrak, T. D. Copeland, C. A. D. Smith, P. T. Mora and S. Oroszlan, Chemical synthesis and expression of the HIV-1 protease gene in E. coli, Biochem. Biophys. Res. Comm. 159: 87 (1989).PubMedCrossRefGoogle Scholar
  3. 3.
    J. M. Louis, C. A. D. Smith, E. M. Wondrak, P. T. Mora and S. Oroszlan, Substitution mutations of the highly conserved Arginine 87 of HIV-1 protease results in loss of proteolytic activity, Biochem. Biophys. Res. Comm. 164: 30 (1989).PubMedCrossRefGoogle Scholar
  4. 4.
    C. Guan, P. Li, P. D. Riggs and H. Inouye, Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein, Gene 67: 21 (1988).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • John M. Louis
    • 1
  • Stephen Oroszlan
    • 2
  • Peter T. Mora
    • 3
  1. 1.Laboratory of Cellular and Developmental BiologyNIDDK, NIHBethesdaUSA
  2. 2.Laboratory of Molecular Virology and Carcinogenesis, ABL-Basic Research ProgramNCI-FCRDCFrederickUSA
  3. 3.Office of the DirectorDCBD, NCI, NIHBethesdaUSA

Personalised recommendations