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Introduction to Fungal Proteinases and Expression in Fungal Systems

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Structure and Function of the Aspartic Proteinases

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 306))

Abstract

Aspartic proteinase production is widespread among the fungi and examples of both secretion and localization in subcellular organelles are known. For example, the yeast Saccharomyces cerevisiae produces aspartic proteinases which reside in the vacuole (proteinase A, analagous to mammalian cathepsin D, Ammerer et al., 1986; Woolford et al., 1986) and within the secretory apparatus (product of the YAP3 gene, Egel-Mitani et al., 1990). It is likely that other fungi produce equivalent proteinases. In addition, yeasts secrete aspartic proteinases such as the barrier proteinase of S. cerevisiae (MacKay et al., 1988; this volume) and the PEPI proteinase of Saccharomycopsis fibuligera (Hirata et al., 1988). The genes for all these proteinases have been cloned and DNA sequences determined from which the amino acid sequences have been derived. Many filamentous fungi are also known to secrete aspartic proteinases and these, as well as secretion of heterologous aspartic proteinase by these fungi, will be the major focus of this chapter.

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References

  • Aikawa, J., Yamashita, T., Nishiyama, M., Horinouchi, S. & Beppu, T., 1990, J. Biol. Chem., 265: 13955–13959.

    PubMed  CAS  Google Scholar 

  • Ammerer, G., Hunter, C. P., Rothman, J. H., Saari, G. C., Vallis, L. A. & Stevens, T. H., 1986, Mol. Cell Biol., 6: 2490–2499.

    PubMed  CAS  Google Scholar 

  • Andreeva, N. S., Zdanov, A. S., Gustchina, A. E. & Federov, A. A., 1984, J. Biol Chem., 259: 11353–11365.

    PubMed  CAS  Google Scholar 

  • Ashikari, T., Amachi, T., Yoshizumi, H., Horiuchi, H., Takagi, M. & Yano, K., 1990, Mol Gen. Genet., 223: 11–16.

    Article  PubMed  CAS  Google Scholar 

  • Barkholt, V., 1987, Eur. J. Biochem., 167: 327–338.

    Article  PubMed  CAS  Google Scholar 

  • Baudys, M., Foundling, S., Pavlík, M., Blundell, T. & Kostka, V., 1988, FEBS Lett., 235: 271–274.

    Article  PubMed  CAS  Google Scholar 

  • Bech, A.-M. & Foltmann, B., 1981, Neth. Milk Dairy J., 35: 275–280.

    CAS  Google Scholar 

  • Berka, R. M., Ward, M., Wilson, L. J., Hayenga, K. J., Kodama, K. H., Carlomagno, L. P. & Thompson, S.A., 1990, Gene, 86: 153–162.

    Article  PubMed  CAS  Google Scholar 

  • Blundell, T., Jenkins, J., Pearl, L., Sewell, T. & Pedersen, V., 1985, in: Aspartic Proteinases and their Inhibitors (Kostka, V., ed.). pp. 151–161. Walter de Gruyter, Berlin.

    Google Scholar 

  • Boel, E., Christensen, T. & Wöldike, H., 1987, European Patent Application, Publication number 0 238 023

    Google Scholar 

  • Bott, R., Subramanian, E. and Davies, D. R., 1982, Biochemistry, 21: 6956–6962.

    Article  PubMed  CAS  Google Scholar 

  • Chen, Z., Han, H. P., Wang, X. J., Koelsch, G., Lin, X. Y., Hartsuck, J. A. & Tang, J., 1991, Manuscript in preparation.

    Google Scholar 

  • Christensen, T., Wöldike, H., Boel, E., Mortensen, S. B., Hjortshoej, K., Thim, L. & Hansen, M. T., 1988, Bioltechnology, 6: 1419–1422.

    Article  CAS  Google Scholar 

  • Cunningham, A., Wang, H.-M, Jones, S. R., Kurosky, A., Rao, L., Harris, C. L, Rhee, S. H. & Hoffmann, T., 1976, Can. J. Biochem., 54: 902–914.

    Article  PubMed  CAS  Google Scholar 

  • Delaney, R., Wong, R. N. S., Meng, G., Wu, N. & Tang, J., 1987, J. Biol. Chem. 262: 1461–1467

    PubMed  CAS  Google Scholar 

  • Dickinson, L., Harboe, M., van Heeswijk, R., Strøman, P. & Jepsen, L. P., 1987, Carlsberg Res. Commun., 52: 243–252.

    Article  CAS  Google Scholar 

  • Egel-Mitani, M., Flygenring, H. P. & Hansen, M. T., 1990, Yeast, 6: 127–137.

    Article  PubMed  CAS  Google Scholar 

  • Emtage, J. S., Angal, S., Doel, M. T., Harris, T. J. R., Jemkins, B., Lilley, G. & Lowe, P. A., 1983, Proc. Natl Acad. Sci. USA, 80: 3671–3675.

    Article  PubMed  CAS  Google Scholar 

  • Foltmann, B., 1986, in: Molecular and Cellular Basis of Digestion (Desnuelle, P., Sjostrom, H. & Noren, O., eds.). pp. 491–505.

    Google Scholar 

  • Foltmann, B., 1988, Biol. Chem. Hoppe-Seyler, 369: 311–314

    PubMed  CAS  Google Scholar 

  • Franke, A. E., Kaczmarek, F. S., Eisenhard, M. E., Geoghegan, K. F., Danley, D. E., De Zeeuw, J. R., O’Donnell, M. M., Gollaher, M. G. Jr. & Davidow, L. S., 1988, in: Developments in Industrial Microbiology, Vol. 29 (Pierce, G., ed.). pp. 43–57. Society for Industrial Microbiology, Arlington.

    Google Scholar 

  • Gilliland, G. L., Winbome, E. L., Nachman, J. & Wlodower, A.., 1990, Proteins, 8: 82–101.

    Article  PubMed  CAS  Google Scholar 

  • Goff, C. G., Moir, D. T., Kohno, T., Gravius, T. C., Smith, R. A., Yamasaki, E. & Taunton-Rigby, A., 1984, Gene, 27: 35–46.

    Article  PubMed  CAS  Google Scholar 

  • Gray, G. L., Hayenga, K., Cullen, D., Wilson, L. J. & Norton, S., 1986, Gene, 48: 41–53.

    Article  PubMed  CAS  Google Scholar 

  • Harrki, A., Uusitalo, J., Bailey, M., Penttil, M. & Knowles, J. K. C., 1989, Bio/technology, 7: 596–603.

    Article  Google Scholar 

  • Hiramatsu, R., Aikawa, J., Horinouchi, S. & Beppu, T., 1989, J. Biol Chem., 264: 16862–16866.

    PubMed  CAS  Google Scholar 

  • Hirata, D., Fukui, S. & Yamashita, I., 1988, Agric. Biol. Chem., 52: 2647–2649.

    Article  CAS  Google Scholar 

  • Horiuchi, H., Yanai, K., Okazaki, T., Takagi, M. & Yano, K., 1988, J. Bacteriol., 170: 272–278.

    PubMed  CAS  Google Scholar 

  • Horiuchi, H., Ashikari, T., Amachi, T., Yoshizumi, H., Takagi, M. & Yano, K., 1990, Agric. Biol. Chem., 54: 1771–1779

    Article  PubMed  CAS  Google Scholar 

  • Innis, M. A., Holland, M. J., McCabe, P. C., Cole, G. E., Wittman, V. P., Tal, R., Watt, K. W. K., Gelfand, D. H., Holland, J. P. & Meade, J. H., 1985, Science, 228: 21–26.

    Article  PubMed  CAS  Google Scholar 

  • James, M. N. G. & Sielecki, A. R., 1983, J. Mol. Biol., 163: 299–361.

    Article  PubMed  CAS  Google Scholar 

  • Jenkins, J., Tickle, I., Sewell, T., Ungaretti, L., Wollmer, A. & Blundell, T., 1977, in: Acid Proteinases, Structure, Function and Biology (Tang, J., ed.). pp. 43–60. Plenum, New York.

    Google Scholar 

  • Kawaguchi, Y., Shimizu, N., Nishimori, K., Uozumi, T. & Beppu, T., 1984, J. Biotechnol., 1: 307–315.

    Article  CAS  Google Scholar 

  • Kawaguchi, Y., Kosugi, S., Sasaki, K., Uozumi, T. & Beppu, T., 1987, Agric. Biol. Chem., 51: 1871–1877.

    Article  CAS  Google Scholar 

  • Kobayashi, H., Sekibata, S., Shibuya, H., Yoshida, S., Kusakabe, I. & Murakami, K., 1989, Agric. Biol. Chem., 53: 1927–1933.

    Article  CAS  Google Scholar 

  • MacKay, V. L., Welch, S. K., Insley, M. Y., Manney, T. R., Holly, J., Saari, G. C. & Parker, M. L., 1988, Proc. Natl. Acad. Sci. USA, 85: 55–59.

    Article  PubMed  CAS  Google Scholar 

  • McCaman, M. T. & Cummings, D. B., 1986, J. Biol. Chem., 261: 15345–15348.

    PubMed  CAS  Google Scholar 

  • McCaman, M. T. & Cummings, D. B., 1988, Proteins, 3: 256–261.

    Article  PubMed  CAS  Google Scholar 

  • McCaman, M. T., Andrews, W. H. & Files, J. G., 1985, J. Biotechnol., 2: 177–190.

    Article  CAS  Google Scholar 

  • Maita, T., Nagata, S., Matsuda, G., Maruta, S., Oda, K., Murao, S. & Tsuru, D., 1984, J. Biochem., 95: 465–475.

    PubMed  CAS  Google Scholar 

  • Mantafounis, D. & Pitts, J., 1990, Prot. Eng., 3: 605–609.

    Article  CAS  Google Scholar 

  • Marston, F. A. O., Lowe, P. A., Doel, M. T., Schoemaker, J. M., White, S. & Angal, S., 1984, Biotechnology, 2: 800–804.

    Article  CAS  Google Scholar 

  • Mellor, J., Dobson, M. J., Roberts, N. A., Tuite, M. F., Emtage, J. S., White, S., Lowe, P. A., Patel, T., Kingsman, A. J. & Kingsman, S. M., 1983, Gene, 24: 1–14.

    Article  PubMed  CAS  Google Scholar 

  • Moir, DS. T., Mao, J., Duncan, M. J., Smith, R. A. & Kohno, T., 1985, in: Developments in Industrial Microbiology, Vol. 26 (Underkoffler, L., ed.). pp. 75–85. Society for Industrial Microbiology, Arlington.

    Google Scholar 

  • Murao, S.& Oda, K., 1985, in: Aspartic Proteinases and their Inhibitors (Kostka, V., ed.). pp. 379–399. Walter de Gruyter, Berlin.

    Google Scholar 

  • Nishimori, K., Shimizu, N., Kawaguchi, Y., Hidaka, M., Uozumi, T. & Beppu, T., 1984, Gene, 29: 41–49.

    Article  PubMed  CAS  Google Scholar 

  • Oda, K. & Murao, S., 1974, Agric. Biol. Chem., 38: 2435–2444.

    Article  CAS  Google Scholar 

  • Ostoslavskaya, V. I., Revina, L. P., Kotlova, E. K., Surova, I. A., Levin, E. D., Timokhina, E. A. & Å tepanov, V. M., 1986, Bioorg. Khim., 8: 1030–1047.

    Google Scholar 

  • Pedersen, V. B., Christensen, K. A. & Foltmann, B., 1979, Eur. J. Biochem., 94: 573–580.

    Article  PubMed  CAS  Google Scholar 

  • Perlman, D. & Halvorson, H. O., 1983, J. Mol. Biol., 167: 391–409.

    Article  PubMed  CAS  Google Scholar 

  • Petrova, E. N., Revina, L. P., Timokhina, E. A., Lavrenova, G. I., Vaganova, T. I. & Å tepanov, V. M., 1987, Biokhimiya, 53: 1389–1396.

    Google Scholar 

  • Smith, R. A., Duncan, M. J. & Moir, D. T., 1985, Science, 229: 1219–1224.

    Article  PubMed  CAS  Google Scholar 

  • Stepanov, V. M., Lavrenova, G. I., Terent’eva, E. Yu., & Khodova, O. M., 1990, FEBS Lett., 260: 173–175.

    Article  PubMed  CAS  Google Scholar 

  • Suguna, K., Bott, R. R., Padlan, E. A., Subramanian, E., Sheriff, S., Cohen, G. H. & Davies, D. R., 1987, J. Mol Biol, 196: 877–900.

    Article  PubMed  CAS  Google Scholar 

  • Suzuki, J., Sasaki, K., Sasao, Y., Hamu, A., Kawasaki, H., Nishiyama, M., Horinouchi, S. & Beppu, T., 1989, Prot. Eng., 2: 563–569.

    Article  CAS  Google Scholar 

  • Takahashi, K., 1987, J. Biol Chem., 262: 1468–1478.

    PubMed  CAS  Google Scholar 

  • Tang, J. & Wong, R. N. S., 1987, J. Cell Biochem., 33: 53–63.

    Article  PubMed  CAS  Google Scholar 

  • Thompson, S. A., 1990, in: Molecular Industrial Mycology: Systems and Applications (Leong, S. A. & Berka, R. M., eds.). in press. Marcel Dekker, New York.

    Google Scholar 

  • Tonouchi, N., Shoun, H., Uozumi, T. & Beppu, T., 1986, Nucl. Acids Res., 14: 7557–7568.

    Article  PubMed  CAS  Google Scholar 

  • Tsujita, Y. & Endo, A., 1976, Biochim. Biophys. Acta, 445: 194–204.

    PubMed  CAS  Google Scholar 

  • Tsujita, Y. & Endo, A., 1977, J. Biochem., 81: 1063–1070.

    PubMed  CAS  Google Scholar 

  • Tsuru, D., Shimada, S., Maruta, S., Yoshimoto, T., Oda, K., Murao, S., Miyata, T. & Iwanaga, S., 1986, J. Biochem., 99: 1537–1539.

    PubMed  CAS  Google Scholar 

  • Tsuru, D., Kobayashi, R., Nakagawa, N. & Yoshimoto, T., 1989a, Agric. Biol. Chem., 53: 1305–1312.

    Article  CAS  Google Scholar 

  • Tsuru, D., Naotsuka, A., Kobayashi, R., Yoshimoto, T., Oda, K. & Murao, S., 1989b, Agric. Biol. Chem., 53: 2751–2756.

    Article  CAS  Google Scholar 

  • van den Berg, J. A., van der Laken, K. J., van Ooyen, A. J. J., Renniers, T. C. H. M., Rietveld, K., Schaap, A., Brake, A. J., Bishop, R. J., Schultz, K., Moyer, D., Richman, M. & Shuster, J. R., 1990, Biol technology, 8: 135–139.

    Google Scholar 

  • Ward, M., 1989a, in; Genetics and Molecular Biology of Industrial Microorganisms (Hershberger, C. L., Queener, S. W. & Hegeman, G., eds.). pp. 288–294. American Society for Microbiology, Washington.

    Google Scholar 

  • Ward, M., 1989b, in: Proceedings of the EMBO-Alko Workshop on Molecular Biology of Filamentous Fungi (Nevalainen, H. & Penttil, M., eds.). pp. 119–128. Foundation for Biotechnical and Industrial Fermentation Research, Helsinki.

    Google Scholar 

  • Ward, M., Wilson, L. J., Kodama, K. H., Rey, M. W. & Berka, R. M., 1990, Bio/technology 8: 435–440.

    Article  PubMed  CAS  Google Scholar 

  • Woolford C. A., Daniels, L. B., Park, F. J., Jones, E. W., van Arsdell, J. N. & Innis M. A., 1986, Mol. Cell. Biol., 6: 2500–2510.

    PubMed  CAS  Google Scholar 

  • Yamashita, T., Tonouchi, N., Uozumi, T. & Beppu, T., 1987, Mol. Gen. Genet. 210: 462–467.

    Article  PubMed  CAS  Google Scholar 

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  1. Genencor International, 180 Kimball Way, South San Francisco, California, 94080, USA

    Michael Ward & Katherine H. Kodama

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  1. Michael Ward
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  2. Katherine H. Kodama
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  1. University of Florida-College of Medicine, Gainesville, Florida, USA

    Ben M. Dunn

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© 1991 Plenum Press, New York

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Ward, M., Kodama, K.H. (1991). Introduction to Fungal Proteinases and Expression in Fungal Systems. In: Dunn, B.M. (eds) Structure and Function of the Aspartic Proteinases. Advances in Experimental Medicine and Biology, vol 306. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-6012-4_20

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  • DOI: https://doi.org/10.1007/978-1-4684-6012-4_20

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-6014-8

  • Online ISBN: 978-1-4684-6012-4

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