Alcoholism pp 115-120 | Cite as

Changes in the Inducibility of a Hepatic Aldehyde Dehydrogenase

  • P. Pappas
  • V. Vasiliou
  • M. Karageorgou
  • M. Marselos
Part of the NATO ASI Series book series (NSSA, volume 206)


Rat liver contains at least two cytosolic aldehyde dehydrogenases (ALDH which are inducible by inducers of drug metabolism (Deitrich, 1971; Marselos and Hänninen, 1974; Deitrich et al., 1977).Phenobarbital (PB) type inducers induce the φ-isozyme in certain rat strains with a genetic predisposition (RR) (Deitrich, 1971; Deitrich et al., 1972; Marselos, 1976; Nakanishi et al., 1978). Polycyclic aromatic hydrocarbons or other type II inducers of drug metabolism can induce another isozyme (τ-ALDH), an isozyme which has been detected in all rat strains tested to date (Deitrich et al., 1978; Marselos et al., 1979; Vasiliou et al., 1988). An increase in ALDH activity was also found in primary cultures of human or rat hepatocytes, after in vitro exposure to PB or methylcholanthrene (Marselos and Michalopoulos, 1986; Marselos et al., 1987).


Polycyclic Aromatic Hydrocarbon Drug Metabolism Aldehyde Dehydrogenase ALDH Activity Glutathione Depletion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Deitrich, R.A., 1971, Genetic aspects of increase in rat liver aldehyde dehydrogenase induced by phenobarbital. Science 173: 334.PubMedCrossRefGoogle Scholar
  2. Deitrich, R.A., Collins, A.C.and Erwin, V.G., 1972, Genetic influence upon phenobarbital-induced increase in rat liver supernatant aldehyde dehydrogenase activity. J. Biol. Chem., 247: 7232–7236.PubMedGoogle Scholar
  3. Deitrich, R.A., Bludeau, P., Stock, T. and Roper, M., 1977, Induction of different rat liver supernatant aldehyde dehydrogenases by phenobarbital and tetrachlorodibenzo-pdioxin. J. Biol. Chem., 252: 6169.PubMedGoogle Scholar
  4. Deitrich, R.A., Bludeau, P., Roper, M. and Schmuck, J., 1978, Induction of aldehyde dehydrogenase. Biochem. Pharmacol., 27: 2343.CrossRefGoogle Scholar
  5. Feinstein, R.N. and Cameron, E.C., 1972, Aldehyde dehydrogenase activity in rat hepatomas. Biochem. Biophys. Res. Commun., 48: 1140.PubMedCrossRefGoogle Scholar
  6. Gornall, A.G., Bardawill, C.J. and David, M.M., 1949, Determinations of serum proteins by means of the biuret reaction. J Biol Chem., 177: 751.PubMedGoogle Scholar
  7. Kamath, S.A., Kummerow, F.A. and Narayan, K.N., 1971, A simple procedure for the isolation of rat liver microsomes. FEBS Lett., 17: 90–92.PubMedCrossRefGoogle Scholar
  8. Kamath, S.A.and Narayan, K.N., 1972, Interaction of Ca with endoplasmic reticulum of rat liver: a standardized procedure for the isolation of rat liver microsomes. Anal. Biochem., 48: 59–61.CrossRefGoogle Scholar
  9. Kosower, N.S. and Kosower, E.M., 1978, The glutathione status of the cell. Int.Rev. Cytol., 54: 109–60.PubMedCrossRefGoogle Scholar
  10. Lindahl, R.and Feinstein, E.C., 1976, Purification and immunochemical characterization of aldehyde dehydrogenase from 2-acetylaminofluorene-induced rat hepatomas. Biochem. Biophys. Acta, 452: 345–355.PubMedGoogle Scholar
  11. Marselos, M.and Hänninen, O., 1974, Enhancement of D-glucuronolactone and aldehyde dehydrogenase activity in the rat liver by inducers of drug metabolism. Biochem. Pharmacol., 24: 1457.CrossRefGoogle Scholar
  12. Marselos, M., 1976, Genetic variation of drug metabolising enzymes in the Wistar rat. Acta Pharmacol. et Toxicol., 39: 186.CrossRefGoogle Scholar
  13. Marselos, M., Törrönen, R., Koivula, T. and Koivusalo, M., 1979, Comparison of phenobarbital-and carcinogen-induced aldehyde dehydrogenase in the rat. Biochem. Biophys. Acta, 583: 110.PubMedCrossRefGoogle Scholar
  14. Marselos, M. and Michalopoulos, G., 1986, Phenobarbital enhances the aldehyde dehydrogenase activity of rat hepatocyte in vitro and in vivo. Acta Pharmacol. et Toxicol., 59: 405.Google Scholar
  15. Marselos, M., Strom, S. and Michalopoulos, G., 1987, Effect of phenobarbital and 3methylcholanthrene on aldehyde dehydrogenase activity in cultures of HepG2 and normal human hepatocytes. Chem. Biol. Interactions, 62: 75.CrossRefGoogle Scholar
  16. Moldeus, P. and Quanguan, J., 1987, Importance of the glutathione cycle in drug metabolism, Pharmacol. Ther., 33: 37–40.PubMedCrossRefGoogle Scholar
  17. Nakanichi, S., Shiobara, E. and Tsukada, M., 1978, Rat liver aldehyde dehydrogenases: strain differences in response of the enzymes to phenobarbital treatment. Jpn. J. Pharmacol., 28: 653–659.CrossRefGoogle Scholar
  18. Reed, DJ. and Fariss, M.W., 1984, Glutathione depletion and susceptibility. Pharmacol. Rev., 36: 25S - 33S.PubMedGoogle Scholar
  19. Reed, DJ., 1986, Regulation of reductive processes by glutathione. Biochem. Pharmacol., 35: 7–13.PubMedCrossRefGoogle Scholar
  20. Ritter, E. and Eriksson, L.C., 1985, Aldehyde dehydrogenase activities in hepatocyte nodules and hepatocellular carcinomas from Wistar rats. Carcinogenesis 6: 1683–1687.PubMedCrossRefGoogle Scholar
  21. Törrönen, R., Nousiainen, U. and Hänninen, O., 1981, Induction of aldehyde dehydrogenase by polycyclic aromatic hydrocarbons in rats. Chem. Biol.Interactions, 36: 33.CrossRefGoogle Scholar
  22. Younes, M., Sharma, S.C. and Siegers, C.-P., 1986, Glutathione depletion by phorone: Organ specificity and effect on hepatic microsomal mixed-function oxidase system. Drug Chem. Toxicol., 9: 67–73.PubMedCrossRefGoogle Scholar
  23. Vasiliou, V., Malamas, M. and Marselos, M., 1986, The mechanism of alcohol intolerance produced by various therapeutic agents. Acta Pharmacol Toxicol., 58: 305–310.CrossRefGoogle Scholar
  24. Vasiliou, V., 1988, The effect of xenobiotics on the hepatic aldehyde dehydrogenase activity. Academic dissertation, University of Ioannina, Greece.Google Scholar
  25. Vasiliou, V., Athanasiou, K. and Marselos, M., 1988, The use of ALDH induction as a carcinogenic risk marker in comparison with a typical in vitro system. In: Travis C.C. (ed): “Biologically Based Methods for Cancer Risk Assessment” New York: Plenum Press, 159: 231–240.Google Scholar
  26. Vasiliou, V. and Marselos, M., 1989, Tissue distribution of inducible aldehyde dehydrogenase activity in the rat after treatment with phenobarbital or 3methylcholanthrene. Pharmacol. Toxicol., 64: 39–42.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • P. Pappas
    • 1
  • V. Vasiliou
    • 1
  • M. Karageorgou
    • 1
  • M. Marselos
    • 1
  1. 1.Department of Pharmacology Medical SchoolUniversity of IoanninaIoanninaGreece

Personalised recommendations