Pro- and Anti-Oxidant Factors in Rat lung Cytosol
During the process of inflammation oxygen radicals are produced by phagocytic cells like neutrophils, eosinophils, monocytes and macrophages. Little is known about the effects of oxygen radicals on lung tissue in inflammatory diseases such as asthma1. Activated phagocytes release Superoxide anions, synthesized by NADPH oxidase which is located in the cellular membrane. Superoxide anions dismutate to hydrogen peroxide enzymatically (superoxide dismutase) or non-enzymatically. These superoxide anions and hydrogen peroxide together form very reactive oxygen species, namely the hydroxyl radical and singlet oxygen. In these reactions iron plays a catalytic role2. Oxygen metabolites cause DNA strand breaks, protein destruction (particularly sulfhydryl groups are sensitive to oxidative stress) and lipid peroxidation. The lipid peroxidation reaction products are very toxic too and cause DNA and protein damage as well3,4.
KeywordsLipid Peroxidation Glutathione Peroxidase Glutathione Reductase Cumene Hydroperoxide Microsomal Lipid Peroxidation
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