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Effects of Singlet Oxygen on the Biological Activity of DNA and Its Involvement in Single Strand-Break Formation

  • Heribert Wefers
  • Paolo Di Mascio
  • Hong-Phuc Do-Thi
  • Dietrich Schulte-Frohlinde
  • Helmut Sies
Part of the Basic Life Sciences book series (BLSC, volume 49)

Abstract

The formation of singlet molecular oxygen (1O2) has been shown to occur during photoexcitation of a variety of biological and also clinically applied compounds, such as porphyrins, psoralens and tetracyclines (see, for example references 1–3), but also occurs during enzymatic reactions including cyclooxygenase4 and chloroperoxidase.5 Regarding the biological consequences of 1O2 formation, DNA is of particular interest. 1O2 is known to react with guanine 6,7; however, results on biologically active DNA were conflicting, in particular regarding 1O2-dependent strand-break formation under neutral conditions, i.e. without alkaline treatment. 8-11 Here we emphasize two aspects of 1O2-mediated damage. First, the effect of 1O2 on the transforming activity of pBR322 and M1 3 DNA in E. coli is presented. Second, two different sources of 1O2 are employed for investigation of strand-break formation; these are microwave discharge and the thermodissociable endoperoxide of disodium 3,3′-(1,4-naphthylidene)dipropionate (NDPO2).

Keywords

Singlet Oxygen Alkaline Treatment Rose Bengal Transforming Activity Cyanuric Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Heribert Wefers
    • 1
  • Paolo Di Mascio
    • 1
  • Hong-Phuc Do-Thi
    • 2
  • Dietrich Schulte-Frohlinde
    • 2
  • Helmut Sies
    • 1
  1. 1.Institut für Physiologische Chemie IUniversität DüsseldorfDeutschland
  2. 2.Max-Planck-Institut für StrahlenchemieMülheim a. d. RuhrDeutschland

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