Abstract
Recently, the assay of lipid hydroperoxides has gained increasing attention since lipid peroxidation products are believed to be involved in the process of aging, mutagenicity and many kinds of disease. For this reason, several methods have been developed for the detection of lipid hydroperoxides in biological materials. These include the thiobarbituric acid method, UV measurement, fluorometric assay, chemiluminescense measurement, and enzymatic assays. However, these methods still have some limitations in their application.
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References
P.T. Gamage, T. Mori, and S. Matsushita, Effects of linoleic acid hydroperoxides and their secondary products on the growth of Escherichia coli, Agric. Biol. Chem. 35:33 (1971).
K. Sohde, S. Izutani, and S. Matsushita, Preparation of triglyceride hydroperoxides, Agric. Biol. Chem. 37:1979 (1973).
P.T. Kissinger, C. Refshauge, R. Dreiling, and R.N. Adams, An electrochemical detector for liquid chromatography with picogram sensitivity, Anal. Lett., 6:465 (1973).
J. Terao, I. Asano, and S. Matsushita, High performance liquid chromatographic determination of phospholipid peroxidation products of rat liver after carbon tetrachloride administration. Arch. Biochem. Biophvs. 235 326–333 (1984).
J. Terao, I. Asano, and S. Matsushita, Preparation of hydroperoxy and hydroxy derivatives of rat liver phosphatidylcholine and phosphatidylethanolamine, Lipids 20:312 (1985).
K. Yamada, J. Terao, and S. Matsushita, Electrochemical detection of phospholipid hydroperoxides in reverse-phase high performance liquid chromatography. Lipids 22:125 (1987).
H. Huges, C.U. Smith, E.C. Horning, and J.R. Mitchell, High-performance liquid chromatography and gas chromatography-mass spectrometry determination of specific lipid peroxidation products in vivo. Anal. Biochem. 130:431 (1983).
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© 1988 Plenum Press, New York
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Matsushita, S., Terao, J., Yamada, K., Shibata, S.S. (1988). Specific Detection of Lipid Hydroperoxides Using HPLC-EC Method. In: Simic, M.G., Taylor, K.A., Ward, J.F., von Sonntag, C. (eds) Oxygen Radicals in Biology and Medicine. Basic Life Sciences, vol 49. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5568-7_24
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DOI: https://doi.org/10.1007/978-1-4684-5568-7_24
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