Specific Detection of Lipid Hydroperoxides Using HPLC-EC Method
Part of the
Basic Life Sciences
book series (BLSC, volume 49)
Recently, the assay of lipid hydroperoxides has gained increasing attention since lipid peroxidation products are believed to be involved in the process of aging, mutagenicity and many kinds of disease. For this reason, several methods have been developed for the detection of lipid hydroperoxides in biological materials. These include the thiobarbituric acid method, UV measurement, fluorometric assay, chemiluminescense measurement, and enzymatic assays. However, these methods still have some limitations in their application.
KeywordsLipid Peroxidation Product Lipid Hydroperoxide Sodium Perchlorate Hydroxy Derivative Linoleic Hydroperoxide
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
P.T. Gamage, T. Mori, and S. Matsushita, Effects of linoleic acid hydroperoxides and their secondary products on the growth of Escherichia coli
, Agric. Biol. Chem.
35:33 (1971).CrossRefGoogle Scholar
K. Sohde, S. Izutani, and S. Matsushita, Preparation of triglyceride hydroperoxides, Agric. Biol. Chem.
37:1979 (1973).CrossRefGoogle Scholar
P.T. Kissinger, C. Refshauge, R. Dreiling, and R.N. Adams, An electrochemical detector for liquid chromatography with picogram sensitivity, Anal. Lett.
, 6:465 (1973).CrossRefGoogle Scholar
J. Terao, I. Asano, and S. Matsushita, High performance liquid chromatographic determination of phospholipid peroxidation products of rat liver after carbon tetrachloride administration. Arch. Biochem. Biophvs.
235 326–333 (1984).CrossRefGoogle Scholar
J. Terao, I. Asano, and S. Matsushita, Preparation of hydroperoxy and hydroxy derivatives of rat liver phosphatidylcholine and phosphatidylethanolamine, Lipids
20:312 (1985).PubMedCrossRefGoogle Scholar
K. Yamada, J. Terao, and S. Matsushita, Electrochemical detection of phospholipid hydroperoxides in reverse-phase high performance liquid chromatography. Lipids
22:125 (1987).PubMedCrossRefGoogle Scholar
H. Huges, C.U. Smith, E.C. Horning, and J.R. Mitchell, High-performance liquid chromatography and gas chromatography-mass spectrometry determination of specific lipid peroxidation products in vivo
. Anal. Biochem.
130:431 (1983).CrossRefGoogle Scholar
© Plenum Press, New York 1988