Identification of Free Radical Species in the Myocardium
With the increasing interest in the role of oxygen-derived free radical generation in various diseases, several attempts to directly evaluate free radicals in tissue have been performed using EPR.1–8 However, none of the previous studies has fully determined the species of the radicals and none has deliberated on the possible existence of artifactual radicals which are likely to be generated during sample handling. The purposes of this study were 1) to establish an artifact-free sampling method for the quantification of freeze-trapped free radicals in myocardium by EPR, 2) to identify artifact radicals and the radicals native to the myocardium and 3) to demonstrate the relationship of these radicals to reperfusion injury.
KeywordsElectron Spin Resonance Rabbit Heart Sodium Diethyldithiocarbamate Myocardial Mitochondrion Japan Electronic Optic Laboratory
Unable to display preview. Download preview PDF.
- 4.N. Yamazaki, Y. Suzuki, N. Kamikawa, and T. Kamikawa, “Studies on myocardial mitochondria in ischemic dog hearts by electron spin resonance (ESR) spectrometry,” University Park Press, Baltimore (1980).Google Scholar
- 8.W. Limm, M. Mugiishi, L. H. Piette, and J.J McNamara, Quantitative assessment of free radical generation during ischemia and reperfusion in the isolated rabbit heart, 4th International Congress on Oxygen Radicals, La Jolla, California, bstract, Thurs., p. 123, (1987).Google Scholar
- 9.S. Hori, H. Nakazawa, A. Murayama, Guillotine freeze-clamping of canine beating heart in short axis plane, Circulation 68 (suppl):323 (1983).Google Scholar
- 10.J. R. Bowyer, and T. Ohnishi, “EPR spectroscopy in the study of ubisemiquinones in redox chains,” Wiley, New York (1985).Google Scholar