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Identification of Free Radical Species in the Myocardium

  • Hiroe Nakazawa
  • Kazunobu Ban
  • Kohji Ichimori
  • Yoshiro Sinozaki
  • Haruka Okino
  • Takashi Masuda
  • Nobuo Aoki
  • Shingo Hori
  • Masahiro Kono
Part of the Basic Life Sciences book series (BLSC, volume 49)

Abstract

With the increasing interest in the role of oxygen-derived free radical generation in various diseases, several attempts to directly evaluate free radicals in tissue have been performed using EPR.1–8 However, none of the previous studies has fully determined the species of the radicals and none has deliberated on the possible existence of artifactual radicals which are likely to be generated during sample handling. The purposes of this study were 1) to establish an artifact-free sampling method for the quantification of freeze-trapped free radicals in myocardium by EPR, 2) to identify artifact radicals and the radicals native to the myocardium and 3) to demonstrate the relationship of these radicals to reperfusion injury.

Keywords

Electron Spin Resonance Rabbit Heart Sodium Diethyldithiocarbamate Myocardial Mitochondrion Japan Electronic Optic Laboratory 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Hiroe Nakazawa
    • 1
  • Kazunobu Ban
    • 1
  • Kohji Ichimori
    • 1
  • Yoshiro Sinozaki
    • 1
  • Haruka Okino
    • 1
  • Takashi Masuda
    • 1
  • Nobuo Aoki
    • 1
  • Shingo Hori
    • 2
  • Masahiro Kono
    • 3
  1. 1.Department of PhysiologyTokai UniversityBohseidai, IseharaJapan
  2. 2.Keio UniversityTokyoJapan
  3. 3.Japan Electronic Optic LaboratoryTokyoJapan

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