The Use of Oxygen Free Radical Scavengers in the Rat Peritonitis Model
In the 1960s, Davis and Yull noted synergistic effect on mortality between E. coli and red blood cells (RBCs) instilled into the peritoneum of rats.1 Specifically, when E. coli at a level of 109 bacteria/cc and hemoglobin (Hb) in a concentration of 4 gm% were injected intraperitone-ally, the 24-hour mortality rate was 90%. When E. coli or hemoglobin was injected separately, there was minimal mortality. This lethal synergistic action has been repeatedly demonstrated as investigators have noted mortality rates of 70 to 90% with this combination.2–5 However, except for the implication of iron as the critical agent,6 little progress has been made in elucidating the adjuvant effect of hemoglobin. Furthermore, no effective treatment has been found.
KeywordsFenton Reaction Abdominal Injury Adjuvant Effect Adjuvant Action Oxygen Free Radical Scavenger
Unable to display preview. Download preview PDF.
- 10.T. Hau, R. Hoffman, and R.L. Simmons, Mechanisms of the adjuvant effect of hemoglobin in experimental peritonits. I. In vivo inhibition of peritoneal leukocytosis, Surgery 83:223 (1978).Google Scholar