Continuous Production of Indole Alkaloids by Gel-Entrapped Cells of Catharanthus Roseus
Plant cell cultures present a great potential for secondary metabolite production (Staba, 1980). Recently, plant cell immobilization techniques have been reported including entrapment in Ca-alginate (Brodelius et al., 1979) and others matrices (Brodelius and Nilsson, 1980; Jirku et al., 1981; Schuler, 1981; Galun et al., 1983; Lindsey et al., 1983), allowing continuous production of useful substances to be obtained. It is obviously important that the working period should be maintained as long is possible with a minimum loss of cell viability and a sustained biosynthetic activity. Besides there is evidence that limitation of growth is essential for the mechanical stability of the Ca-alginate matrix and often results in enhanced production of metabolites (Lindsey and Yeoman, 1983).
KeywordsCell Suspension Culture Plant Cell Culture Indole Alkaloid FEBS Letter Catharanthus Roseus
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