Plant Vacuoles pp 495-501 | Cite as

Continuous Production of Indole Alkaloids by Gel-Entrapped Cells of Catharanthus Roseus

  • Alain Pareilleux
  • Florence Majerus
Part of the NATO ASI Series book series (NSSA, volume 134)


Plant cell cultures present a great potential for secondary metabolite production (Staba, 1980). Recently, plant cell immobilization techniques have been reported including entrapment in Ca-alginate (Brodelius et al., 1979) and others matrices (Brodelius and Nilsson, 1980; Jirku et al., 1981; Schuler, 1981; Galun et al., 1983; Lindsey et al., 1983), allowing continuous production of useful substances to be obtained. It is obviously important that the working period should be maintained as long is possible with a minimum loss of cell viability and a sustained biosynthetic activity. Besides there is evidence that limitation of growth is essential for the mechanical stability of the Ca-alginate matrix and often results in enhanced production of metabolites (Lindsey and Yeoman, 1983).


Cell Suspension Culture Plant Cell Culture Indole Alkaloid FEBS Letter Catharanthus Roseus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Brodelius, P., Deus, B., Mosbach, K., and Zenk, M. H., 1979, Immobilized plant cells for the production and transformation of natural products, FEBS Letters, 103: 93.PubMedCrossRefGoogle Scholar
  2. Brodelius, P., and Nilsson, K., 1980, Entrapment of plant cells in different matrices. A comparative study, FEBS Letters, 122: 312.CrossRefGoogle Scholar
  3. Galun, E., Aviv, D., Dantes, A., and Freeman, A., 1983, Planta Medica, 49: 9.PubMedCrossRefGoogle Scholar
  4. Gamborg, O. L., Miller, R. A., and Ojima, K., 1968, Nutrient requirements of suspension cultures of soybean root cells, Exptl. Cell Res., 50: 151.PubMedCrossRefGoogle Scholar
  5. Jirku, V., Mocek, T., Vanek, T., Krumphanzl, V., and Kubanek, V., 1981, Continuous production of steroid glycoalkaloids by immobilized plant cells, Biotechnol. Letters, 3: 447.CrossRefGoogle Scholar
  6. Knobloch, K. H., Beutnagel, G., and Berlin, J., 1981, Influence of accumulated phosphate on culture growth and formation of cinnamoyl-putrescines in medium-induced cell suspension cultures of Nicotiana tabacum, Planta, 153: 582.CrossRefGoogle Scholar
  7. Knobloch, K. H., Hansen, B., and Berlin, J., 1981, Medium-induced formation of indole-alkaloids and concomitant changes of interrelated enzyme activities in cell suspension cultures of Catharanthus roseus, Z. Naturforsch., 36c: 40.Google Scholar
  8. Knobloch, K. H., and Berlin, J., 1983, Influence of phosphate on the formation of indole-alkaloids and phenolic compounds in cell suspension cultures of Catharanthus roseus: 1. Comparison of enzyme activities and product accumulation, Plant Cell Tissue Organ. Cult., 2: 333.CrossRefGoogle Scholar
  9. Lindsey, K., and Yeoman, M. M., 1983, Novel experimental systems for studying the production of secondary metabolites by plant tissue cultures, in: “Soc. Exptl. Biol. Sem. Ser.”, Vol. 18: Plant Biotechnology, pp. 39–66, S. H. Man-tell and H. Smith, eds., Cambridge University Press, Cambridge.Google Scholar
  10. Lindsey, K., and Yeoman, M. M., 1983, The relationship between growth rate, differentiation and alkaloid accumulation in cell cultures, J. Exptl. Bot., 34: 1055.CrossRefGoogle Scholar
  11. Lindsey, K., Yeoman, M. M., Black, G. M., and Mavituna, F., 1983, A novel method for the immobilization and culture of plant cells, FEBS Letters, 155: 143.CrossRefGoogle Scholar
  12. Majerus, F., and Pareilleux, A., 1986 a, Plant Cell Reports, accepted.Google Scholar
  13. Majerus, F., and Pareilleux, A., 1986 b, Biotechnol. Letters, submitted.Google Scholar
  14. Neumann, D., Krauss, G., Hieke, M., and Groger, D., 1983, Indole alkaloid formation and storage in cell suspension cultures of Catharanthus roseus, Planta Med., 48: 2 0.Google Scholar
  15. Renaudin, J. P., and Guern, J., 1982, Compartmentation mechanisms of indole-alkaloids in cell suspension cultures of Catharanthus roseus, Physiol. Vég., 2 0: 533.Google Scholar
  16. Schuler, M. L., 1981, Production of secondary metabolites from plant cell cultures. Problems and prospects, Ann. N. Y. Acad. Sci., 369: 65.CrossRefGoogle Scholar
  17. Staba, E. J., 1980, Secondary metabolism and biotransformation, in: “Plant Tissue Culture as a Source of Biochemicals”, E. J. Staba, ed., CRC Press Inc., Boca-Raton, pp. 59–98.Google Scholar
  18. Vinas, R., and Pareilleux, A., 1982, Production d’alcaloïdes par des suspensions cellulaires de Catharanthus roseus cultivées in vitro, Physiol. Vég., 20: 219.Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Alain Pareilleux
    • 1
  • Florence Majerus
    • 1
  1. 1.Département de Génie Biochimique et AlimentaireInstitut National des Sciences AppliquéesToulouse-CedexFrance

Personalised recommendations