Prospects for Cellular Mutational Assays in Human Populations
Practical, sensitive, effective, human cellular assays for detecting somatic and germinal mutations would have great value in environmental mutagenesis and carcinogenesis. When available, such assays should allow us to fill the void between human mutagenicity and the data that exist from short-term tests and from mutagenicity in other species. We will be able to validate the role of somatic mutation in carcinogenesis, to identify environmental factors that affect human germ cells, to integrate the effects of complex mixtures and the environment in the human subject, and to identify people who are hypersusceptible to genetic injury. Human cellular mutational assays, particularly when combined with cytogenetic and heritable mutational tests, promise to play pivotal roles in estimating the risk from low-dose radiation and chemical exposures. These combined methods avoid extrapolations of dose and from species to species, and may be sensitive enough and credible enough to permit politically, socially and scientifically acceptable risk management.
KeywordsLactate Glycine Germinal Serine Leucine
Unable to display preview. Download preview PDF.
- Albertini, R.J., 1982, Studies with T-lymphocytes: An approach to human mutagenicity monitoring, in: “Banbury Report 13, Indicators of Genotoxic Exposure,” pp. 393–412, B.A. Bridges, B.E. Butterworth, and I.B. Weinstein, eds., Cold Spring Harbor Lab.Google Scholar
- Bigbee, W.L., Langlois, R.G., Vanderlaan, M., and Jensen, R.H., 1984, Binding specificities of eight monoclonal antibodies to human glycophorin A: Studies using MCM and MkEn(UK) variant human erythrocytes and M- and MNV-type chimpanzee erythrocytes, J. Immunol. (in press).Google Scholar
- Jones, I.M., Burkhart-Schultz, K., and Carrano, A.V., 1984, Cloning of thioguanine resistant lymphocytes for measurement of in vivo mutation in the mouse (in preparation).Google Scholar
- Stamatoyannopoulos, G., Nute, P.E., Papayannopoulou, Th., McGuire T.C., Lim, G., Bunn, H.F., and Rucknagel, D., 1980, Development of a somatic mutation screening system using Hb mutants. IV. Successful detection of red cells containing the human frameshift mutants Hb Wayne and Hb Cranston using monospecific fluorescent antibodies, Am. J. Hum. Genet., 32:484.PubMedGoogle Scholar