Abstract
One of the active areas in cell biology involves the attempt to identify, characterize, and localize cytoskeletal components in nonmuscle cells. The standard approaches in this field have been the detection of a protein by gel electrophoresis or chromatography, the purification to homogeneity, the preparation of specific antibodies, and the localization by immunocytochem-istry. The number of cytoskeleton-associated proteins is on the order of 1000. The rate-limiting step has been the isolation of individual components to a sufficient purity for raising highly specific antibodies.
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© 1985 Plenum Press, New York
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Chen, L.B., Rosenberg, S., Nadakavukaren, K.K., Walker, E.S., Shepherd, E.L., Steele, G.D. (1985). The Cytoskeleton. In: Springer, T.A. (eds) Hybridoma Technology in the Biosciences and Medicine. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4964-8_15
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DOI: https://doi.org/10.1007/978-1-4684-4964-8_15
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