Demonstration of Receptor Function of Membrane Proteins by Selection and Immobilization with Monoclonal Antibodies
Monoclonal antibodies (MAbs) that are specific for certain types of cells and for stages of ontogenetic development can be produced without prior knowledge of antigenic differences between the cell types. Once identified, however, the elucidation of the function of these interesting antigens may represent a formidable task. In this chapter, we discuss the technique of selection and immobilization of membrane proteins by MAbs bound to Staphylococcus aureus bacteria. This technique allows visualization of receptor interactions with ligands on cells or in solution. Since multivalent interactions between S. aureus bacteria-MAb-receptor complexes and ligand-coated particles can be established, the method is particularly useful for studies on receptors where univalent reactions are of low affinity. The use of this method to identify two different membrane proteins as receptors for fragments of the third component of complement (C3) and one of these additionally as an Epstein-Barr virus (EBV) receptor, is described. To introduce these studies, the biology of C3, C3 receptors, and EBV is first briefly reviewed.
KeywordsSystemic Lupus Erythematosus Receptor Function Complement Receptor Ligand Binding Site Sheep Erythrocyte
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