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Adenosine Deaminase

Measurement of Activity and Use of Inhibitors

  • Chapter
Methods Used in Adenosine Research

Abstract

The physiologic and pharmacologic importance of purines, adenosine, and their analogs as regulators of blood flow, lipolysis, neurotransmission, and immune functions and their antineoplastic activity has been reviewed in this volume and elsewhere (Burnstock, 1981; Schubert et al., 1979; Suhadolnik, 1970). The enzyme adenosine deaminase (ADA; adenosine aminohydrolase, EC 3.5.4.4), which plays an important role in the regulation of adenosine and its analogs, is the subject of the present discussion.

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References

  • Adamson, R. H., Zaharevitz, D. W., and Johns, D. G. 1977. Enhancement of the biological activity of adenosine analogs by the adenosine deaminase inhibitor 2’-deoxy coformycin. Pharmacology, 75: 84–89.

    Article  Google Scholar 

  • Agarwal, R. P. 1979. Recovery of 2’-deoxycoformycin-inhibited adenosine deaminase of mouse eryth-rocytes and leukemia L1210 in vivo. Cancer Res, 39: 1425–1427.

    PubMed  CAS  Google Scholar 

  • Agarwal, R. P. 1980. In vivo inhibition of adenosine deaminase by 2’-deoxycoformycin in mouse blood and leukemia LI210 cells. Biochem. Pharmacol, 29: 187–193.

    Article  PubMed  CAS  Google Scholar 

  • Agarwal, R. P. 1982. Inhibitors of adenosine deaminase. Pharmacol. Ther. 17:399–429. Agarwal, R. P., Sagar, S. M., and Parks, R. E., Jr. 1975. Adenosine deaminase from human erythrocytes: Purification and effects of adenosine analogs. Biochem. Pharmacol, 24: 693–701.

    Google Scholar 

  • Agarwal, R. P., Crabtree, G. W., Parks, R. E., Jr., Nelson, J. A. Keightley, R., Parkman, R., Rosen, R. Stern, R. C., and Polmar, S. H. 1976. Purine nucleoside metabolism in the erythrocytes of patients with adenosine deaminase deficiency and severe combined immunodeficiency. J. Clin. Invest, 57: 1025–1035.

    Article  PubMed  CAS  Google Scholar 

  • Agarwal, R. P., Spector, T., and Parks, R. E., Jr. 1977. Tight-binding inhibitors IV: Inhibition of adenosine deaminase by various inhibitors. Biochem. Pharmacol, 26: 359–367.

    Article  PubMed  CAS  Google Scholar 

  • Agarwal, R. P., Major, P. P., and Kufe, D. W. 1982a. Simple and rapid high-performance liquid chromatographic method for analysis of nucleosides in biological fluids. J. Chromatogr, 231: 418–424.

    Article  PubMed  CAS  Google Scholar 

  • Agarwal, R. P., Blatt, J., Miser, J., Sallan, S., Lipton, J. M., Reaman, G. H., Holcenberg, J., and Poplack, D. G. 1982b. Clinical pharmacology of 9-β-D arabinofuranosyl adenine in combination with 2’-deoxycoformycin. Cancer Res, 42: 3884–3886.

    PubMed  CAS  Google Scholar 

  • Arch, J. R. S. and Newsholme, E. A. 1978. Activities and some properties of 5’-nucleotidase, adenosine kinase and adenosine deaminase in tissues from vertebrates in relation to the control of the concentration and the physiological role of adenosine. Biochem. J, 774: 965–977.

    Google Scholar 

  • Anderson, E. P. 1973. Nucleoside and nucleotide kinases. The Enzymes, 9: 49–96.

    Article  CAS  Google Scholar 

  • Baker, D. C., Hanvey, J. C., Hawkins, L. D. and Murphy, J. 1981. Identification of the bioactive enantiomer of erythro-3-(adenine-9yl)-2-nonanol (EHNA), a semi-tight binding inhibitor of adenosine deaminase. Biochem. Pharmacol, 30: 1159–1160.

    Article  PubMed  CAS  Google Scholar 

  • Barton, R., Martiniuk, F., Hirschhorn, R., and Goldschneider, I. 1980. Inverse relationship between adenosine deaminase and purine nucleoside phosphorylase in rat lymphocyte population. Cell. Immunol, 49: 208–214.

    Article  PubMed  CAS  Google Scholar 

  • Bastian, G., Bessodes, M., Panzica, R. P., Abushanab, E., Chen, S. F., Stoeckler, J. D. and Parks, R. E., Jr. 1981. Adenosine deaminase inhibitors. The conversion of a single chiral synthon into erythro and threo-9-(2-hydroxy-3 nonyl) adenine. J. Med. Chem, 24: 1383–1384.

    Article  PubMed  CAS  Google Scholar 

  • Ben-Basset, I., Simoni, F., Holtzman, F., and Ramot, B. 1979. Adenosine deaminase activity of normal lymphocytes and leukemic cells. Israel J. Med. Sci, 75: 925–927.

    Google Scholar 

  • Bessodes, M. D., Bastian, G., Abushanab, E., Panzica, R. P., Berman, S. F., Marcaccio, E. J., Jr., Chen, S. F., Stoeckler, J. D., and Parks, Jr. 1981. Effect of chirality in erythro-9-(2-hydroxy-3- nonyl) adenine. Biochem. Pharmacol, 37: 879–882.

    Google Scholar 

  • Blatt, J., Reaman, G., Poplack, D. G. 1980. Biochemical markers in lymphoid malignancy. N. Engl. J. Med, 303: 918–922.

    Article  PubMed  CAS  Google Scholar 

  • Burnstock, G. 1981. Neurotransmitters and trophic factors in the autonomic nervous system. J. Physiol, 5 /3: 1–35.

    Google Scholar 

  • Caron, N., Lee S. H., and Kimball, A. P. 1977. Effect of 2’-deoxycoformycin, 9-(3-D-arabinofura- nosyladenine-5’-phosphate, and l-p-D-arabinofuranosylcytosine triple combination therapy on intracerebral leukemia 1210. Cancer Res, 37: 3274–3279.

    PubMed  CAS  Google Scholar 

  • Cass, C. E., and Au-Yeung, T. H. 1976. Enhancement of 9-(3-D-arabinofuranosyl adenine cytotoxicity to mouse leukemia L1210 in vitro by 2’-deoxycoformycin. Cancer Res, 36: 1486–1491.

    PubMed  CAS  Google Scholar 

  • Cha, S. 1975. Tight-binding inhibitors: I. Kinetic behavior. Biochem. Pharmacol, 24: 2177–2185.

    Article  PubMed  CAS  Google Scholar 

  • Cha, S. 1976. Tight-binding inhibitors: III. A new approach for the determination of competition between tight-binding inhibitors and substrates—inhibition of adenosine deaminase by cofor- mycin. Biochem. Pharmacol, 25: 2695–2702.

    Article  PubMed  CAS  Google Scholar 

  • Cha, S., Agarwal, R. P. and Parks, R. E., Jr. 1975. Tight-binding inhibitors: II. Non-steady state nature of inhibition of milk xanthine oxidase by allopurinol and alloxanthine and of human erythrocytic adenosine deaminase by coformycin. Biochem. Pharmacol, 24: 2187–2197.

    Article  PubMed  CAS  Google Scholar 

  • Chaney, A. L., and Marbach, E. P. 1962. Modified reagents for determination of urea and ammonia. Clin. Chem, 5: 130–132.

    Google Scholar 

  • Chassin, M. M., Chirigos, M. A., Johns, D. G. and Adamson, R. H. 1977. Adenosine deaminase inhibition for immunosuppressive. N. Engl. J. Med, 296: 1232.

    Google Scholar 

  • Coleman, M. S., Greenwood, M. F., Hutton, J. J., Holland, P., Lampkin, B., Krill, C., and Kastelic, J. E. 1978. Adenosine deaminase, terminal deoxynucleotidyl transferase (TdT), and cell surface markers in childhood acute leukemia. Blood, 52: 1125–1131.

    PubMed  CAS  Google Scholar 

  • Conner, J. D., Sweetman, L., Carey, S., Stuckey, M. A., and Buchanan, R. 1974. Effect of adenosine deaminase upon the antiviral activity in vitro of adenine arabinoside for vaccinia virus. Antimicrob. Ag. Chemother, 6: 630–636.

    Article  Google Scholar 

  • Dissing, J., and Knudsen, B. 1972. Adenosine deaminase deficiency and combined immunodeficiency syndrome. Lancet, 2: 1316.

    Article  PubMed  CAS  Google Scholar 

  • Evans, B., and Wolfenden, R. 1970. A potential transition-state analog for adenosine deaminase. J. Am. Chem. Soc, 92: 4751–4752.

    Article  PubMed  CAS  Google Scholar 

  • Falcon, M. G., and Jones, B. R. 1977. Antiviral activity in the rabbit cornea of adenine arabinoside, Ara-A 5’-monophosphate, and hypoxanthine arabinoside; and interaction with adenosine deaminase inhibitor. J. Gen. Virol, 36: 199–202.

    Article  PubMed  CAS  Google Scholar 

  • Giblett, E. R., Anderson, J. E., Cohen, F., Pollara, B., and Meuwissen, H. J. 1972. Adenosine deaminase deficiency in two patients with severely impaired cellular immunity. Lancet, 2: 1067–1069.

    Article  PubMed  CAS  Google Scholar 

  • Glazer, R. I., and Peale, A. L. 1978. Cordycepin and xylosyladenine: Inhibitors of methylation of nuclear RNA. Biochem. Biophys. Res. Commun, 57: 521–526.

    Article  Google Scholar 

  • Grever, M., Coleman, M. S., Balcerzak, S. P. 1983. Adenosine deaminase and terminal deoxynu- cleotidyl transferase: biochemical markers in the management of chronic myelogeneous leukemia. Cancer Res, 43: 1442–1445.

    PubMed  CAS  Google Scholar 

  • Hartwick, R., Jeffries, A., Krstulovic, A., and Brown, P. R. 1978. An optimized assay for adenosine deaminase using reverse phase high pressure liquid chromatography. J. Chromatgr, 76: 427–435.

    Google Scholar 

  • Hopkinson, D. A., Cook, P. J. L., and Harris, H. 1969. Further data on the adenosine deaminase (ADA) polymorphism and a report of a new phenotype. Ann. Hum. Genet, 52: 361–367.

    Article  Google Scholar 

  • Kedar, A., Tritsch, G. L., Freeman, A. I. 1980. Transient stimulatory adenosine deaminase activity in peripheral lymphocytic lysate from a case of T-cell lymphoma. Res. Commun. Chem. Pathol. Pharmacol, 28: 153–162.

    PubMed  CAS  Google Scholar 

  • Koehler, L. H., and Benz, E. J. 1962. Serum adenosine deaminase: Methodology and clinical application. Clin. Chem, 8: 133–140.

    PubMed  CAS  Google Scholar 

  • Killer, C. A., Mitchell, B. S., Grever, M. R., Mejais, E., Malspeis, L.,andMetz, E. N. 1979. Treatment of acute lymphoblastic leukemia with 2’-deoxycoformycin: Clinical and biochemical consequences of adenosine deaminase (EC 3.5.4.4) inhibition. Cancer Treat. Rep., 65: 1949–1952.

    Google Scholar 

  • Koya, M., Kansh, T., Sawada, H., Uchino, H., and Neda, K. 1981. Adenosine deaminase and ecto- 5’-nucleotidase activities in various leukemias with special reference to blast crisis of chronic myeloid leukemia. Blood, 55: 1107–1111.

    Google Scholar 

  • Kvamme, E., Tveit, B., and Svennedy, G. 1965. Glutaminase from pig kidney, an allossteric protein. Biochem. Biophys. Res. Commun, 20: 566–572.

    Article  PubMed  CAS  Google Scholar 

  • Lee, C. H., Evan, S. P., Rosenberg, M. C., Bagnara, A. S., Ziegler, J. B., and Van der Wyden, M. D. 1979. In vitro platelet abnormality in adenosine deaminase deficiency and severe combined immunodeficiency. Blood, 53: 465–471.

    PubMed  CAS  Google Scholar 

  • Lum, C. T., Sutherland, D. E. R., Yasmineh, W. G., and Najarian, J. S. 1978. Peripheral blood mononuclear cell adenosine deaminase activity in renal allograft recipients. J. Surg. Res, 24: 388–395.

    Article  PubMed  CAS  Google Scholar 

  • Lum, C. T., Sutherland, D. E. R., Yashmineh, D. S., Howard, R. J., and Najarian, J. S. 1979. Adenosine deaminase activity in cytomegolovirus related graft and patient loss. Transplantation Proc, 77: 83–88.

    Google Scholar 

  • Major, P. P., Agarwal, R. P., and Kufe, D. W. 1981a. Deoxycoformycin: Neurological toxicity. Cancer Chemother. Pharmacol, 5: 193–196.

    Article  PubMed  CAS  Google Scholar 

  • Major, P. P., Agarwal, R. P., and Kufe, D. W. 1981b. Clinical pharmacology of deoxycoformycin. Blood, 58: 91–96.

    PubMed  CAS  Google Scholar 

  • Major, P. P., Agarwal, R. P., and Kufe, D. W. 1983. Clinical pharmacology of arabinosyladenine in combination with deoxycoformycin. Cancer Chemother. Pharmacol, 70: 125–128.

    Article  Google Scholar 

  • Meier, J., Coleman, M. S., and Hutton, J. J. 1976. Adenosine deaminase activity in peripheral blood cells of patients with hematologic malignancies. Br. J. Cancer, 55: 312–319.

    Article  Google Scholar 

  • Meuwissen, H. J., Pollara, B., and Pickering, R. J. 1975. Combined immunodeficiency disease associated with adenosine deaminase. J. Pediatr, 86: 169–181.

    Article  PubMed  CAS  Google Scholar 

  • Miwa, S., Fuji, H., Matsumoto, N., Nakatsuji, T., Oda, S., Asano, H., Asano, S., and Miura, Y. 1978. A case of red cell adenosine deaminase over production associated with hereditary hemolytic anemia found in Japan. Am. J. Hematol, 5: 107–115.

    Article  PubMed  CAS  Google Scholar 

  • Muller, W. E. G., Zahn, R. K., Arendez, J., Maidhof, A., and Umezawa, H. 1978. Influence of coformycin on the cytostatic activity of 9-(3-D-Arabinofuranosyladenine and adenosine in mouse L5178Y cells. Hoppe-Seyler’s Z. Physiol. Chem, 559: 1287–1295.

    Article  Google Scholar 

  • Nakamura, H., Koyama, G., Iitaka, Y., Ohno, M., Yagisawa, N., Kondo, S., Maeda, K., and Umezawa, H. 1974. Structure of coformycin, an unusual nucleoside of microbial origin. J. Am. Chem. Soc, 96: 4327–4328.

    Article  PubMed  CAS  Google Scholar 

  • Nakamura, H., Koyama, G., Umezawa, H., and Iitaka, Y. 1976. The crystal and molecular structure of coformycin. Acta Cryst, B32: 1206–1212.

    Article  Google Scholar 

  • Newby, A. C. 1980. Role of adenosine deaminase, ecto (5’-nucleotidase) and ecto (non-specific phosphatase) in cyanide-induced adenosine monophosphate catabolism in rat polymorphonuclear leucocytes. Biochem. J, 756: 907–918.

    Google Scholar 

  • Nishihara, H., Akedo, H., Okada, H., and Hattori, S. 1970. Multienzyme patterns of serum adenosine deaminase by agar gel electrophoresis. Clinica. Chim. Acta, 30: 251–258.

    Article  CAS  Google Scholar 

  • Nishihara, H., Ishikawa, S., Shinkai, K., and Akedo, H. 1973. Multiple forms of human adenosine deaminase. Biochem. Biophys. Acta, 302: 429–442.

    Article  PubMed  CAS  Google Scholar 

  • North, T. W., and Cohen, S. S. 1978. Erythro-9-(2-hydroxy-3-nonyl) adenine as a specific inhibitor of herpes simplex virus replication in the presence and absence of adenosine analogues. Proc. Nat. Acad. Sci. USA, 75: 4684–4688.

    Article  PubMed  CAS  Google Scholar 

  • Nygaard, P. 1978. Adenosine deaminase from Escherichia coli. Methods Enzymol, 57: 508–512.

    Article  Google Scholar 

  • Orfanos, A. P., Nylor, E. W., and Guthrie, R. 1978. Micromethod for estimating adenosine deaminase activity in dried blood spots on filter paper. Clin. Chem, 24: 591–594.

    PubMed  CAS  Google Scholar 

  • Parkman, R. G., Gelfand, F. W., Rosen, F., Sanderson, A., and Hirschhorn, R. 1975. Severe combined immunodeficiency disease associated with adenosine deaminase deficiency. N. Engl. J. Med, 292: 714–719.

    Article  PubMed  CAS  Google Scholar 

  • Parks, R. E., Jr., and Brown, P. R. 1973. Incorporation of nucleosides into the nucleotide pools of human erythrocytes, adenosine and its analogs. Biochemistry, 12: 3294–3302.

    Article  PubMed  CAS  Google Scholar 

  • Pauling, L. 1948. Chemical achievement and hope for the future. Am. Scientist, 16: 51–58.

    Google Scholar 

  • Polmar, S. H., Stern, R. C., Schwartz, A. L., Wetzler, E. M., Chase, P. A., and Hirschhorn, R. 1976. Enzyme replacement therapy for adenosine deaminase deficiency and severe combined immunodeficiency. TV. Engl. J. Med, 295: 1337–1343.

    Article  CAS  Google Scholar 

  • Prentice, H. G., Smyth, J. F., Ganeshguru, K., Wonk, B., Bradstock, K. F., Janossy, G., Goldstone, A. K., and Hoffbrand, A. V. 1980. Remission induction with adenosine deaminase inhibitor 2’- deoxycoformycin in Thy-lymphoblastic leukemia. Lancet. 2: 170–172.

    Article  PubMed  CAS  Google Scholar 

  • Rodbard, D., Bridson, W., and Rayford, P. L. 1969. Rapid calculation of radioimmunoassay results. Lab. Clin. Med, 74: 110–118.

    Google Scholar 

  • Rogler-Brown, T., Agarwal, R. P., and Parks, R. E., Jr. 1978. Tight-binding inhibitors. VI. Interactions of deoxycoformycin and adenosine deaminase in intact human enthrocytes and Sarcoma 180 cells. Biochem. Pharmacol, 27: 2289–2296.

    Article  PubMed  CAS  Google Scholar 

  • Sawa, T., Fukagawa, Y., Homma, I., Takeuchi, T., and Umezawa, H. 1967a. Mode of inhibition of coformycin on adenosine deaminase. J. Antiobiotics (Japan) Ser.A, 20: 221–231.

    Google Scholar 

  • Sawa, T., Fukagawa, Y., Homma, I., Takeuchi, T., and Umezawa, H. 1967b. Formycin deaminating activity of microorganisms. J. Antibiotics (Japan) Ser. A, 20: 317–321.

    CAS  Google Scholar 

  • Schaeffer, H. J., 1971. Factors in the design of reversible and irreversible enzyme inhibitors. In: Drug Design: pp. 129–159. Medicinal Chemistry, Volume 11-Pt. II Ed. by Ariens, E. J. Academic Press, New York.

    Google Scholar 

  • Schaeffer, H. J., and Schwender, C. F. 1974. Enzyme inhibitors. 26. Bridging hydrophobic regions on adenosine deaminase with some 9-(2-hydroxy-3-alkyl) adenines. J. Med. Chem, 77: 6–8.

    Article  Google Scholar 

  • Scholar, E. M., and Calabresi, P. 1973. Identification of the enzymic pathway of nucleotide metabolism in human lymphocytes and leukemia cells. Cancer Res, 33: 94–103.

    PubMed  CAS  Google Scholar 

  • Schroder, H. C. Schuster, D. K., Zahn, R. K., and Muller, W. E. G. 1981. A novel metabolic effect of the adenosine deaminase inhibitor coformycin, a potentiator of antiviral adenosine analogues. Antiviral Res, 7: 383–391.

    Google Scholar 

  • Schubert, P., Reddington, M., and Kreutzberg, G. W. 1979. On the possible role of adenosine as a modulatory messenger in the hippocampus and other regions of the CNS. Prog. Brain Res, 57: 149–165.

    Article  Google Scholar 

  • Seligson, D., and Seligson, H. 1951. A microdiffusion method for the determination of nitrogen liberated as ammonia. J. Lab. Clin. Med, 8: 324–330.

    Google Scholar 

  • Shimazaki, M., Kondo, S., Maeda, K., Ohno, M., and Umezawa, H. 1979a. Synthesis of isocoformycin, an aadenosine deaminase inhibitor of synthetic origin. J. Antibiotics (Japan), 12: 537–538.

    Article  Google Scholar 

  • Shimazaki, M., Kumada, Y., Takeuchi, T., Umezawa, H., and Watanabe, K. 1979b. Studies on inhibition of adenosine deaminase by isocoformycin in vitro and in vivo. J. Antibiotics (Japan), 32: 654–658.

    Article  CAS  Google Scholar 

  • Sidi, Y., Boer, P. Pick, I., Pinkhas, J., Sperling, O. 1979. Increased adenosine deaminase activity in peripheral lymphocytes in Waldenstrom’s macroglubulinaemia. Lancet, 7: 500.

    Article  Google Scholar 

  • Sloan, B. J., Kielty, J. K., and Miller, F. A. 1977. Effect of a novel adenosine deaminase inhibitor (Co-vidarabine, CoV) upon the antiviral activity in vitro and in vivo of vidarabin (Vira-Aâ„¢) for DNA viral replication. Ann. N.Y. Acad. Sci, 284: 60–80.

    Article  PubMed  CAS  Google Scholar 

  • Smyth, J. F., and Harrap, K. R. 1975. Adenosine deaminase in leukemia. Br. J. Cancer, 31: 544–549.

    Article  PubMed  CAS  Google Scholar 

  • Smyth, J. F., Poplack, D. G., Holiman, B. J., and Leventhal, B. G. 1978. Correlation of adenosine deaminase activity with cell surface markers in acute lymphoblastic leukemia. J. Clin. Invest, 62: 710–712.

    Article  PubMed  CAS  Google Scholar 

  • Smyth, J. F., Chassin, M. M., Harrap, K. R. x Adamson, R. H., and Johns, D. G. 1979. 2’-Deoxy- coformycin (DCF): Phase I trial and clinical pharmacology. Proc. Am. Assoc. Cancer Res, 20: 41.

    Google Scholar 

  • Stubbs, G., Litt, M., Lis, E., Jackson, R., Voth, W., Lindberg, A., and Litt, R. 1982. Adenosine deaminase activity decreased in autism. J. Am. Acad. Child. Psych, 27: 71–74.

    Google Scholar 

  • Sufrin, G., Tritsch, G., Mittleman, A., Moore, R. H., and Murphy, G. P. 1977. Adenosine deaminase activity in patients with renal adenocarcinoma. Cancer, 40: 796–802.

    Article  PubMed  CAS  Google Scholar 

  • Sufrin, G., Tritsch, G. L., Mittleman, A., and Murphy, G. P. 1978. Studies of lymphocyte adenosine deaminase activity in patients with renal and transitional cell carcinoma. Int. Adv. Surg. Oncol, 7: 11–28.

    Google Scholar 

  • Suhadolnik, R. J. 1970. Nucleoside Antibiotics. Wiley, New York.

    Google Scholar 

  • Tritsch, G. L., and Minowada, J. 1978. Differences in purine metabolizing enzyme activities in human leukemia T-cell, B-cell and null-cell lines. J. Natl. Cancer Inst, 60: 1301–1304.

    PubMed  CAS  Google Scholar 

  • Trotta, P. P., and Balis, M. E. 1977. Structural and kinetic alterations in adenosine deaminase as-sociated with the differentiation of rat intestinal cells. Cancer Res, 37: 2297–2305.

    PubMed  CAS  Google Scholar 

  • Trotta, P. P., Tedde, A., and Balis, M. E. 1980. Effects on immune function of continuous infusion into mice of 2’-deoxycoformycin (DCF). Proc. Am. Assoc. Cancer Res, 21: 242.

    Google Scholar 

  • Uitendaal, M. P., DeBruyn, C. H. M. M., Oei, T. L., Geerts, S. J., and Hosli, P. 1978. Fluctuating adenosine deaminase activities in cultured fibroblasts, Biochem. Med, 20: 54–62.

    Article  CAS  Google Scholar 

  • Vaca, G., Sanchez-Corona, J., Olivares, N., Medina, C., Ibara, B., and Cantu, J. M. 1979. A simple rapid fluorescent assay for adenosine deaminase activity. Ann. Genet, 22: 182–184.

    PubMed  CAS  Google Scholar 

  • Wierzchowski, J., and Shugar, D. 1983. Sensitive fluorimetric assay for adenosine deaminase with formycin as substrate; and substrate and inhibitor properties of some pyrazolopyrimidine and related analogues. Z. Naturforsch, 38c: 61–13.

    Google Scholar 

  • Wiginton, D. A., Coleman, M. S., and Hutton, J. J. 1981. Purification, characterization and radioim-munoassay of adenosine deaminase from human leukemic granulocytes Biochem. J, 195: 389–397.

    PubMed  CAS  Google Scholar 

  • Woo, P. W. K., Dion, H. W., Lang, S. M., Dahl, L. F., and Durham, L. J. 1974. A novel adenosine and Ara-A deaminase inhibitor (R)-3-(2-deox-β-D-erythropentofuranosyl)-3,6,7,8-tetrahydroimi-dazo-[4,5-d] [1,3] diazepin-8-ol.

    Google Scholar 

  • Zielke, C. L., and Suelter, C. H. 1971. Purine nucleoside, and purine nucleotide aminohydrolases. The Enzymes, 4: 41–18.

    Article  Google Scholar 

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Authors and Affiliations

  1. Section of Medical Oncology Evans Memorial Department of Clinical Research, Boston University Medical Center, Boston, Massachusetts, USA

    Ram P. Agarwal

  2. Departments of Medicine and Pharmacology, Boston University Medical Center, Boston, Massachusetts, USA

    Ram P. Agarwal

  3. Hubert H. Humphrey Cancer Research Center, Boston University Medical Center, Boston, Massachusetts, USA

    Ram P. Agarwal

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  1. Ram P. Agarwal
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  1. University of Auckland, Auckland, New Zealand

    David M. Paton

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Agarwal, R.P. (1985). Adenosine Deaminase. In: Paton, D.M. (eds) Methods Used in Adenosine Research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4886-3_6

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  • DOI: https://doi.org/10.1007/978-1-4684-4886-3_6

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