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Regeneration of Plants from Crown Gall Cells A step in the T-DNA mediated genetic engineering of plants

  • A. Wöstemeyer
  • L. Otten
  • H. De Greve
  • J. P. Hernalsteens
  • J. Leemans
  • M. Van Montagu
  • J. Schell
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 61)

Abstract

The mechanism whereby the plant pathogenic soil bacterium Ag-robacterium tumefaciens induces Crown Gall plant tumors is the result of a remarkable example of naturally occurring genetic engineering. A fragment of about 20 kb (called the T-region) of a 120 kb plasmid (called Ti plasmid for tumor-inducing plasmid) is transferred to the plant cell where it becomes covalently linked to the nuclear DNA (1–7). This integrated DNA has been named the T-DNA. Crown Gall cells have two distinctive properties: They grow without hormones (8,9) and they produce so-called opines: compounds not found in normal plant cells and used specifically by Agrobacterium tumefaciens as a carbon, nitrogen and energy source with the help of a Ti plasmid-encoded uptake and degradation system (10–13). Opines moreover have the capacity to induce a plasmid transfer system which can spread the opine degradation system through an Agrobacterium population (14,15). For recent reviews see 3, 16–20.

Keywords

Tobacco Protoplast Strain B6S3 Numerous Shoot Crown Gall Tissue Hemizygous State 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • A. Wöstemeyer
    • 1
  • L. Otten
    • 1
  • H. De Greve
    • 2
  • J. P. Hernalsteens
    • 3
  • J. Leemans
    • 3
  • M. Van Montagu
    • 3
  • J. Schell
    • 1
    • 2
  1. 1.Max-Planck-Institut für ZüchtungsforschungKölnDeutschland
  2. 2.Laboratory for GeneticsState-University-GentBelgium
  3. 3.Laboratory for Genetical VirologyFree University of BrusselsBelgium

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