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The Major B and T Cell Determinant on Pigeon Cytochrome c in B10.A Mice

  • Charles Hannum
  • Michiel Ultee
  • Louis A. Matis
  • Ronald H. Schwartz
  • E. Margoliash
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 150)

Abstract

The specificities of B10.A B and T cells responding to pigeon cytochrome c have been examined. Proliferating T cells recognize glutamine 100 and lysine 104 and can be stimulated in vitro by either native cytochrome c molecules or certain of their CNBr-cleavage fragments. In contrast only molecules with the native cytochrome c conformation were found to interact with B10.A anti-pigeon cytochrome c antibodies. Antibodies appear to recognize a determinant or determinants which overlaps with that which elicits the T cell proliferative response.

Keywords

Antigenic Determinant Cation Exchange Chromatography Competitive Binding Assay Tobacco Hornworm Native Cytochrome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Charles Hannum
    • 1
  • Michiel Ultee
    • 1
  • Louis A. Matis
    • 2
  • Ronald H. Schwartz
    • 2
  • E. Margoliash
    • 1
  1. 1.Department of Biochemistry, Molecular, and Cell BiologyNorthwestern UniversityEvanstonUSA
  2. 2.The Laboratory of Immunology, National Institute of Allergy and Infectious DiseasesNational Institutes of HealthBethesdaUSA

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